RAPD技术对地方鸡种群体遗传结构的分析

利用ＲＡＰＤ技术对４个地方鸡种和１个引进鸡种的群体遗传结构进行分析，通过筛选的５个随机引物ＯＰＨ－０２、ＯＰＨ－０５、ＯＰＨ－１３、ＯＰＨ－１６、ＯＰＧ－０７对５个鸡种的池ＤＮＡ进行多态性研究，结果表明：５个引物共产生条带６１个，扩增产物片段的长度一般从１５０ｂｐ－４ｋｂ。     

小菜蛾Toll-6基因的克隆及功能分析

为探究Toll-6基因在小菜蛾Plutella xylostella先天免疫中的功能,对克隆鉴定的Toll-6基因序列进行生物信息学分析,利用实时荧光定量PCR检测Toll-6基因的时空表达模式及微生物侵染响应模式,体外验证其在微生物结合、病原相关分子模式（pathogen associated molecular pattern,PAMP）结合和细菌凝集等方面的功能。结果表明：Toll-6基因全长2 313 bp,编码770个氨基酸,预测Toll-6蛋白具有富含亮氨酸重复序列（leucine-rich repeats,LRR）胞外区、跨膜区和Toll/白介素-1受体同源（Toll/interleukin-1 receptor homologous,TIR）胞内等典型Toll受体家族特征;Toll-6基因在不同发育阶段及不同组织中均有表达,其中成虫期表达量最高,4龄幼虫期次之,在4龄幼虫中肠表达量最高;此外,苏云金芽胞杆菌Bacillus thuringiensis菌株Bt8010侵染小菜蛾4龄幼虫6 h后,Toll-6基因表达被显著抑制,而黏质沙雷氏菌Serratia marcesc...     

喷克防治苹果主要病害应用技术

喷克（ｍａｎｃｏｚｅｂ）８０％可湿性粉剂６００倍,８００倍,１０００倍室内对苹果轮纹（Ｐｈｙｓａｌｏｓｐｏｒａｐｉｒｉｃｏｌａ）抑菌效果均达１００％,６００倍,００倍对苹果斑点落叶病菌（Ａｌｔｅｒｎａｒｉａｍａｌｉ）炭疽病菌（Ｇｌｏｍｅｒｅｌｌａｃｉｎｇｕｌａｔａ）亦具显著的抑制效果,人工接菌条件下,８００倍液防治苹果斑点落叶病的实际保护期主１０－１２ｄ,防治苹果轮纹病的有效保护期为８－１０ｄ。田     

水稻磷酸核酮糖激酶基因OsPRK克隆、亚细胞定位与诱导表达分析

为探究水稻磷酸核酮糖激酶基因OsPRK在水稻诱导抗虫反应中的功能,以水稻秀水110为材料克隆OsPRK基因的全长,通过生物信息学软件分析其序列特征,并应用实时荧光定量PCR技术分析OsPRK基因在水稻不同组织中的分布情况以及在虫害诱导、激素和机械损伤处理水稻中的表达特征。结果显示,水稻OsPRK基因序列全长为1 212 bp,编码403个氨基酸,分子量为44.86 kD,具有1个磷酸核酮糖激酶保守结构域。OsPRK蛋白亚细胞定位结果显示其定位于叶绿体。OsPRK基因在水稻中的表达具有组织特异性,其在内叶、外叶、内叶鞘、外叶鞘和根系这5个组织中相对于内参基因ACTIN的表达量分别为35.83、20.53、6.25、3.21和0.03。与对照相比,二化螟Chilo suppressalis为害能够强烈抑制水稻茎秆中OsPRK基因的表达;褐飞虱Nilaparvata lugens怀卵雌成虫为害1.5、24 h、白背飞虱Sogatella furcifera怀卵雌成虫为害1、8、24 h以及机械损伤处理3、6、24 h均能显著诱导水稻茎秆中OsPRK基因的表达;而OsPRK基因的表达量在茉莉酸处理6、12 h时以及水杨酸处理0.5、1.5 h时被显著抑制,在茉莉酸处理48 h和水杨酸处理24 h时被显著诱导。表明OsPRK基因可能参与了水稻对害虫的诱导防御反应。     

Changes in the relationship between species richness and belowground biomass among grassland types and along environmental gradients in Xinjiang,Northwest China

The association between biodiversity and belowground biomass (BGB) remains a central debate in ecology. In this study, we compared the variations in species richness (SR) and BGB as well as their interaction in the top (0-20 cm), middle (20-50 cm) and deep (50-100 cm) soil depths among 8 grassland types (lowland meadow, temperate desert, temperate desert steppe, temperate steppe desert, temperate steppe, temperate meadow steppe, mountain meadow and alpine steppe) and along environmental gradients (elevation, energy condition (annual mean temperature (AMT) and potential evapotranspiration (PET)), and mean annual precipitation (MAP)) based on a 2011-2013 survey of 379 sites in Xinjiang, Northwest China. The SR and BGB varied among the grassland types. The alpine steppe had a medium level of SR but the highest BGB in the top soil depth, whereas the lowland meadow had the lowest SR but the highest BGB in the middle and deep soil depths. The SR and BGB in the different soil depths were tightly associated with elevation, MAP and energy condition; however, the particular forms of trends in SR and BGB depended on environmental factors and soil depths. The relationship between SR and BGB was unimodal in the top soil depth, but SR was positively related with BGB in the middle soil depth. Although elevation, MAP, energy condition and SR had significant effects on BGB, the variations in BGB in the top soil depth were mostly determined by elevation, and those in the middle and deep soil depths were mainly affected by energy condition. These findings highlight the importance of environmental factors in the regulations of SR and BGB as well as their interaction in the grasslands in Xinjiang.     

机械化深施肥技术及推广

机械化深施肥技术是作物施肥作业的技术创新,可提高作业效率、肥料利用率和作物产量.但机械化深施肥技术要达到普及推广,还须采取多种措施:加大推广示范和政策扶持力度,加强深施肥技术的农机与农艺配套研究,研究制定机械施肥作业技术标准;农机制造企业要提高产品性能和质量,提高设备使用的可靠性;同时,化肥生产企业也应参与研究,共同开发与深施技术配套的肥料.     

引入联想教育理念铸就现代职教模式——计算机应用专业校企合作联想专班项目建设典型案例

近年来,在国家大力发展职业教育的系列政策的推动下,职业教育取得了长足发展.职校与联想集团共同探索和推进校企融合、教产一体的工学结合人才培养模式,通过联想专班建设,建立 IT人才培养标准与创新人才培养方案,从而探索职业教育培养模式及职教改革路径.     

香菇简易开放式栽培高产菌株的早期鉴定

试验对５个香菇菌株进行简易开放式栽培并观察其特性，发现菌丝生长速度、锁状联合数与生物学效率相关性不密切，香菇的酶活性，子实体形成所需时间、子实体单重、菌盖重与生物学效率密切相关。     

An analysis of structure of tree seedling populations on a Lahar

The structure of a tree seedling population is dependent on the interaction of several processes including seed dispersal, germination, survival, and competition on a physical landscape. Structural components (composition, size distributions, spatial distributions, age distributions, density, and history) of a tree seedling population on the Muddy River Lahar on the east side of Mount St. Helens were examined over a range of extents (1/10 m to 1000 m). Many of these component have rarely been examined at the larger extents listed here. Composition reflected distances to seed source and seed morphology. Seedling sizes are inversely proportional to depth to a buried soil if one existed. Spatial patterns indicated that seedling are clustered for tree seedlings less than 200 m apart, random for tree seedling from 200 m to 400 m and uniform for seedling greater than 400 m apart. This was confirmed by two measures of multidimensional spatial point pattern. Age distributions did not reflect the size distributions; old seedlings could be almost any size, young seedlings were constrained to be small in size. Densities appear to be typical for forests in the area. History of disturbance events (the lahar establishment, and successive ash, pumice, and erosion) has strongly influenced this tree seedling community.     

鸡腿蘑菌丝体的同工酶研究

用8种不同培养基配方，培养鸡腿蘑菌丝体，其同工酶分析结果表明，鸡腿蘑菌丝体酯酶(EST)同工酶谱差异明显，过氧化物酶(POD)同工酶谱差异明显。