Impact of different crop rotations and tillage systems on weed infestation and productivity of bread wheat

Crop rotation and tillage systems have important implications for weed infestation and crop productivity. In this study, five tillage systems viz. zero tillage (ZT), conventional tillage (CT), deep tillage (DT), bed sowing (60/30 cm with four rows; BS₁) and bed sowing (90/45 cm with six rows; BS₂) were evaluated in five different crop rotations viz. fallow-wheat (FW), rice-wheat (RW), cotton-wheat (CW), mungbean-wheat (MW) and sorghum-wheat (SW) for their effect on weed infestation and productivity of bread wheat. Interaction between different tillage practices and cropping systems had significant effect on density and dry biomass of total, broadleaved and grass weeds, agronomic and yield-related traits, and grain yield of bread wheat. The un-disturbed soils (ZT) under fallow-wheat or mungbean-wheat rotations favoured the weed prevalence (a total weed dry biomass of 72.4–109.6 and 105.6–112.1 g m⁻² in first and second year, respectively). Contrary to this, the disturbed soils (CT, DT, BS₁ and BS₂) had less weed infestation with either of the rotations (a total weed biomass of 0.4–7.1 and 1.1–5.4 g m⁻² in first and second year, respectively). Sorghum-wheat rotation had strong suppressive effect on weed infestation in all tillage systems. The impact of crop rotation was more visible during second year of experimentation. Bed sown wheat (BS₁ and BS₂) in mungbean-wheat rotation had the highest wheat grain yield (6.30–6.47 t ha⁻¹) compared to other tillage systems in different crop rotation combinations.     

An improved method for extraction of sorghum polymeric protein complexes

A method for fractionating sorghum proteins using extraction solvents and techniques designed to obtain polymeric protein structures (especially disulfide linked) was developed. Extraction and separation conditions were optimized in terms of completeness of protein extraction, sample stability, and analytical resolution. After pre-extraction of albumins and globulins, a 3-step sequential procedure involving no reducing agents was applied to ground whole sorghum flour. The three fractions obtained represented proportionally different protein polymer contents and molecular weight distribution as evidenced by comparative size exclusion chromatography. Protein composition also varied among the extracts with differences in kafirin composition and non-kafirin proteins detected in the fractions by RP-HPLC and SDS-PAGE analysis. The ability to quantify and further characterize sorghum polymeric protein complexes will be useful for additional studies linking protein structures with functionality and digestibility and variations for these properties within diverse sorghum germplasm.     

浅议保护性耕作技术推广过程中存在的问题及解决途径

土地采用保护性耕作方法,可有效的防止水土流失,增加土壤蓄水能力,提高雨水利用率,培肥地利,增加土壤有机质,减少耕作次数,降低生产成本,各种农作物增产明显。     

增强学生对“作物栽培学实验”学习兴趣的探讨

该文根据在作物栽培学及其实验的教学过程中的心得体会,从实验教学内容和模式、教学手段、教学方法及考核办法等几个方面展开论述,总结了如何增强学生对该实验课程的学习兴趣,以便更好地改善教学质量。     

基质分散萃取-高效液相色谱法对菠菜中噻虫胺残留的检测

研究确立了乙腈为分散萃取溶剂,以羧基化双壁碳纳米管(DWCNTs-COOH)和石墨化碳黑(GCB)为基质分散材料的菠菜(Spinacia oleracea L.)样品中噻虫胺残留基质分散萃取前处理方法,建立并优化了乙腈等度洗脱的HPLC残留样品外标定量方法。结果表明,优化色谱条件下,噻虫胺标准样品的色谱保留时间为6.258 min,在0.05~100 mg/L范围内与对应色谱峰积分面积线性响应良好,回归方程为y=4.948 2x-1.316 9(R~2=0.999 7),噻虫胺在菠菜样品中0.1、0.5和5.0 mg/kg 3个水平的添加回收率均在85%以上,各添加水平的相对标准偏差均小于7%,该色谱条件下仪器检出限为0.052 7μg/L,方法的最低检测量为0.015 mg/kg。表明该残留样本前处理方法和样品检测方法简便、高效、经济、可靠,可满足噻虫胺在菠菜中的残留定量检测要求。     

秸秆深还机具特点和作业效率分析

秸秆深埋还田(秸秆深还)作为一种新兴的秸秆还田模式,其机具有别于地表覆盖还田和铧式犁翻压还田。为了科学管理、合理运用秸秆深还机械,提高机组的作业效率,在调查测试秸秆深还作业的基础上,对机具的特点进行了分析,同时对测试机组分别进行了时间利用率和作业效率分析。结果表明:对于各个机具的作业时间利用率和总作业效率影响因素有所不同。作业速度、幅宽、故障维修和地头转向是影响机械作业效率的主要因素。根据调查和分析的结果,针对秸秆深还作业中存在的问题提出了机组的合理运行模式。     

秸秆覆盖与镇压对小黑麦生长状况的影响

以小黑麦作为研究材料,通过设置5种不同的秸秆覆盖与镇压处理,在小黑麦的各个生长期对其进行生理指标的测定,研究秸秆覆盖与镇压对小黑麦鲜重和干物质重、株高、次生根、叶片数的影响.试验结果表明:5种处理下小黑麦的生长状况呈现出了:A4＞ A3＞ A＞ A1＞ A5.而且A4处理下小黑麦的生长状况明显大于其他各组处理下小黑麦的...     

Nanostructured liquid crystalline particle assisted delivery of 2,4-dichlorophenoxyacetic acid to weeds,crops and model plants

Routine agricultural practices are heavily dependent on the use of surfactants, many of which are toxic to humans and detrimental to the environment. In proof of concept work we have previously shown the potential of nanostructured liquid crystalline particles (NLCP) to safely interact with plant leaf cuticular surfaces with minimal impact on epicuticular waxes. Here we demonstrate the use of NLCP to effectively deliver the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) to plant leaves in laboratory and field studies. In the laboratory, the physiological stress responses of lupin, Lupinus angustifolius (L.) (Fabaceae) towards NLCP spray applications were shown to be much reduced in comparison with application of two common surfactants. Phytotoxicity assays of 2,4-D loaded NLCP were used to validate the herbicidal effects on Arabidopsis thaliana (L.) Heynth. (Brassicaceae) and established a similarity with that of surfactant assisted 2,4-D delivery when tested at a concentration of 0.1%. Field trials were conducted to test the efficacy of NLCP-assisted delivery of 2,4-D in comparison with commercial surfactants for the control of the invasive weed wild radish, Raphanus raphanistrum (L.) (Brassicaceae), in wheat, Triticum aestivum (L.) (Poaceae) crop fields. Compared against Estercide 800, a commercially available 2,4-D formulation, NLCP assisted delivery of 2,4-D was effective at low concentrations of 0.03% and 0.06%. The crop yield remained similar for all the tested concentrations and formulations of 2,4-D loaded NLCP and Estercide 800. This is the first report to directly show that, as an alternative to conventional methods, NLCP can be used under both laboratory and field conditions to successfully delivery an agrochemical.     

Farmers' knowledge and perception of grain legume pests and their management in the Eastern province of Kenya

Grain legumes play an important role in community livelihood and in the national economy in Kenya. Unfortunately, in many African countries, production doesn't satisfy the demand in grains due to various constrains. Understanding farmers practices and behavior in the management of grain legume pests is a crucial step in the development of sustainable management strategies. A total of 216 farmers were surveyed in eight districts of eastern Kenya to evaluate farmers' knowledge and perceptions of grain legume pests; to examine current pest management practices, and to identify other production constraints. Grain legumes are grown by a wide age-group of farmers, with both genders equally represented. Chemical control remains the main pest management strategy, and, to ensure pesticide effectiveness, farmers also use increased application rates, chemical alternation, frequent application and mixtures of chemicals. While farmers used other control measures, they showed only limited interest in biological control. The majority of the farmers had experience in grain legume farming and were able to identify the major pests, which were the legume flower thrips Megalurothrips sjostedti Trybom, the cowpea aphid Aphis craccivora Koch and the legume pod borer Maruca vitrata Fabricius. Our survey revealed that education and proximity to extension services contributed significantly to farmers' knowledge of grain legume pests, suggesting the need to provide continuous training and capacity building on integrated pest management in grain legume farming. The study also suggests integration of other pest management strategies such as the use of early maturing varieties, biopesticides and biofertilizer to reduce the use of chemical for sustainable pest management.     

高效液相色谱法测定海洋生物中牛磺酸的含量

[目的]建立牛磺酸的高效液相色谱分析方法,并通过该方法测定不同海洋生物中牛磺酸的含量。[方法]应用邻苯二甲醛(OPA)柱前衍生法,研究流动相比例、流速、衍生剂比例、衍生时间等因素对牛磺酸检测的影响。[结果]当牛磺酸∶OPA=1∶2,衍生时间2 min,流动相乙腈∶水=15∶85,流速为0.5 mL/min,检测波长为360 nm时,牛磺酸浓度为10~400μg/mL时具有良好的线性关系(R~20.99),平行样品的相对标准偏差为2.9%~7.4%。利用该方法检测不同海产品中牛磺酸的含量,所选择的海洋贝类、甲壳类、鱼类中均含有牛磺酸,其中海瓜子中含量最高,达11.2 mg/g。不同组织比较,鱼肉中牛磺酸含量较高,而鳃中较低。[结论]该研究结果丰富了海洋生物中牛磺酸含量的数据,为进一步研究牛磺酸在不同组织中的合成机制提供了参考。