农杆菌介导的pac1基因转化大豆研究

选用高感大豆花叶病毒病而再生能力较强的大豆品种和品系作为受体,对丛生芽诱导培养基、侵染菌液的制备以及根的诱导方法进行了优化。采用优化后的遗传转化体系以农杆菌介导法将pac1导入大豆,MSB培养基中附加2 mg/L6-BA+0.2 mg/LIBA进行丛生芽诱导,液体YEB重悬活化菌体后侵染,MSB培养基中蔗糖含量15%+2 mg/LIBA+0.2 mg/L6-BA进行生根诱导。共获得8株PCR检测呈阳性植株,经SouthernBlot检测,证明pac1基因已整合到大豆基因组中。转基因植株对病毒的抗性评价正在进行中。     

基于WSN的温室环境监测节点设计

设计了一种带扩展板的无线传感器网络监测节点,节点以CC2430为核心,采用ZigBee技术实现无线传感器网络自组网和监测数据的自动汇聚,实现了无线传输、自适应组网、自动发现新加节点和失效节点、传感器类型可扩展、实时密集监测等功能,且具有功耗小、成本低、精度高、稳定性好等优点。     

长白猪肝脏、肾脏和淋巴结的显微形态及糖原PAS反应研究

[目的]对商品长白猪的组织形态学进行研究,了解商品长白猪宰前的生理状态。[方法]采集长白猪的肝脏、肾脏和淋巴结3种组织器官并制成石蜡切片,采用H.E染色方法对其组织结构进行研究。[结果]肾脏和肝脏的组织结构形态正常,肾脏和肝脏的细胞核染色明显,淋巴结的皮质和髓质分界不清晰,周围组织没有明显的淋巴索,淋巴窦较小。糖原PAS染色结果表明肝脏中的糖原颗粒较少,分布不均匀,而肾脏中糖原或糖蛋白多集中于血管球和肾小囊壁上。[结论]该研究可为今后进一步的商品长白猪理论研究奠定基础。     

大豆子叶节高效再生系统的研究

为建立一个大豆子叶节高效再生体系用于大豆的遗传转化,以桂春豆1号、桂早2号、桂夏1号和桂夏豆2号4个大豆品种为材料,子叶节为外植体诱导丛生芽,再生完整植株.实验研究了大豆种子的消毒方法,基因型以及培养过程中植物激素的浓度等影响大豆子叶节再生的因素.结果表明在适宜的条件下,4个大豆品种的丛生芽分化率都可达到90％,丛生芽数基本都在4～6个范围内,4个大豆品种均为子叶节器官发生途径的理想基因型.最适合的种子灭菌方法是双氧水灭菌法;桂春豆1号和桂早2号的适宜芽诱导培养基为B5+ 6-BA 1.6 mg/L+ IBA 0.2 mg/L,桂夏1号和桂夏豆2号为B5 +6-BA 1.0 mg/L +IBA 0.2 mg/L;4个大豆品种的适宜丛生芽伸长培养基为1/2 MS+B5有机+GA3 0.5 mg/L;生根培养基为B5 +NAA0.5 mg/L+ 2.0 g/L活性炭.     

定植和打顶及品种对雾培马铃薯器官建成的影响

该试验采用L9(34)正交试验设计,研究了不同定植节数、打顶次数及品种对雾培马铃薯植株茎粗、根系、LAI,LOX活性及结薯能力的影响.结果表明:雾培马铃薯定植3节及打顶2次处理均能促进LOX活性增强、匍匐茎发生,增加块茎数量和质量;结薯能力与品种差异相关密切,品种费乌瑞它结薯能力最佳;同时发现LOX活性增强利于雾培马铃薯结薯.品种费乌瑞它结合定植3节和打顶2次为雾培结薯最优组合.     

UNUSUAL DISC HERNIATION IN A DOG

Unusual disc herniation was identified in a dog. Disc herniation was considered unusual because of its displacement into the vertebral endplate of the adjoining vertebra. Unusual disc herniation in this dog was compared with Schmorl's node in humans.     

Flowering-pattern and yield components at inflorescence nodes of snap bean as affected by irrigation and plant density

This paper reports the results of a 2 × 2 factorial experiment on bush snap beans ‘Oregon 1604’. The treatments were 2 contrasted irrigation regimes and 2 contrasted plant densities, and were applied in 1978 and repeated in 1979. Data were collected on the number of flowers and pods, and pod size, at each node of the terminal inflorescence (6-T) of the main stem, and at each node of the oldest inflorescence (2-A) at Node 2. High and low plant densities were 45 and 18 plants m^?2 in 1978 and 54 and 33 plants m^?2 in 1979. High temperatures, frequently above 32°C, prevailed during bloom and pod development in 1978, but for the most part occurred only during the week prior to bloom in 1979. Inflorescences 6-T and 2-A usually formed 4 and 3 RN's, respectively, in 1978 and 3 and 2 RN's in 1979. The flowers at the proximal nodes of each inflorescence all opened within a few days of one another (duration of flowering at proximal nodes between 3 and 5 days); the flowering-periods of adjacent nodes overlapped, and the flowering period increased acropetally within the inflorescence (duration of flowering at distal nodes between 7 and 13 days). In general, number of flowers, pods formed, pods harvested and percent set decreased acropetally within each inflorescence. The rate of acropetal decline was lessened by high irrigation or low plant density. In both years, high irrigation increased the percent set of all RN's of the 2-A inflorescences, but few other consistent effects between years were observed. The 2 most proximal RN's together produced 93% or more of the yield of each inflorescence. High irrigation significantly increased the total number of pods harvested from these RN's of inflorescences 6-T and 2-A, and low density had a similar effect on 2-A.     

Evaluation of the Abbott LCx Mycobacterium Tuberculosis Assay for Direct Detection of Mycobacterium Bovis in Bovine Tissue Samples

The commercial LCx amplification assay, usually employed to detect the Myocobacterium tuberculosis complex in respiratory specimens, was evaluated by comparing the results it gave with those obtained using Löwenstein-Jensen solid medium and pathological findings on 55 lymph nodes from cattle with positive and 10 lymph nodes from cattle with negative skin tests for tuberculosis. Fifty-three cultures (51 and 2, respectively) were positive for M. bovis, while the results for the LCx assay and the histological method were positive in 48 (45, 3) and 24 (20, 4) samples, respectively. None of the samples from cattle from certified tuberculosis-free herds were positive by any of the procedures. The results obtained with the LCx assay, compared with the culture procedure, regarded as the gold standard among the diagnostic techniques, gave a specificity of 91.6% and sensitivity of 90.5%. Although the sensitivity of LCx was suboptimal, DNA of M. bovis was detected in 81.8% of the skin test-positive animals. Amplification techniques could provide a rapid and reasonably reliable tool for detecting bovine tuberculosis.     

葡萄实生树开花节位与内源激素变化的关系

 采用酶联免疫法( ELISA) 分析了巨峰葡萄2年生自然实生树不同节位芽中内源赤霉素(GA₃和GA_{4 + 7} ) 、吲哚乙酸( IAA) 、脱落酸(ABA) 、玉米素核苷(ZR) 和异戊基腺嘌呤( iPA) 的含量; 并对实生树自然条件下最低成花节位进行了调查。结果表明, 2年生巨峰实生树在20节左右进入生殖生长阶段, GA₃和GA_{4 + 7}的含量随着节位升高而降低, ABA的含量却急剧增加, 但在第31～35节又降到很低;ABA /GA₃和ABA /GA_{4 + 7}在16～20 节前变化不大, 而在此之后, 都一致表现出上升的趋势, 这反映了以GA₃或GA_{4 + 7}为基数的比值, 可以更明显地反应激素的变化及其与巨峰葡萄阶段转变的关系。     

无线传感器网络的移动节点定位算法研究

利用物体运动的连续性,将移动节点的运动规律与距离测量相结合提出了运动预测定位算法,该算法不需要额外的硬件支持,适应能力强,在信标节点密度比较低时提高了性能.