肥城桃两品系果实细胞壁成分和水解酶活性的比较

 随着果实发育成熟，肥城桃两品系果实中细胞壁含量不断下降。可溶性果胶从8月1～16日明显增加，这时果肉硬度下降最快。两品系果实的细胞壁中离子结合果胶和纤维素含量下降比例不同，这可能是造成两品系成熟软化特性不同的主要因素之一。果实发育后期‘白里肥城桃’和‘红里肥城桃’纤维素酶活性变化的不同，意味着纤维素酶在肥城桃果实软化中起重要作用。肥城桃果实中没有检测到内切PG活性，成熟后期外切PG活性上升较快。     

社会性别视角下的农村家庭养老研究

以家庭养老为主的农村,女性参与养老所发挥的作用与其非主体身份极为不对称,本文从社会性别视角探讨隐含的影响因素及运作机制,认为社会性别规范通过经济、制度和文化等3个层面对女性以主体身份平等参与养老发挥影响,且影响是连续的,其最终的解决不单靠政府的力量,还应从根本上重视社会性别作为一种文化模式的影响。     

木薯细胞壁酸性转化酶MeCWINCV5基因启动子的克隆和序列分析

[目的]了解MeCWINCV5在植物生长发育、激素调节和逆境胁迫应答中的功能.[方法]采用PCR方法从木薯基因组中分离MeCWINCV5基因启动子序列,考察MeCWINCV5对植物生长发育、激素调节和逆境胁迫应答的影响.[结果]分析显示启动子的长度为1170bp,合有TATA box和CAAT box等多个典型的真核生物启动子基本元件元件,还存在大量逆境胁迫诱导相关的顺式调控元件,如HSE、MBS、SARE、GARE-motif和TATC-box等多个与植物逆境胁迫相关的元件.[结论]MeCWINCV5基因启动子与逆境胁迫有关,在木薯抵御逆境胁迫的生理过程中具有重要作用.     

小鼠上皮细胞培养及其在EOB研究中的应用

小鼠眼睑发育需要表皮角质形成细胞的增殖、分化、凋亡及迁移的协调作用。表皮角质形成细胞是研究眼睑闭合不全机制重要的细胞模型。表皮角质形成细胞培养时,贴壁差,增殖力低,生活力弱,生长条件要求高,传代次数有限。对近年来表皮角质形成细胞的体外培养方法及其在遗传性眼睑闭合不全研究中的应用进行综述,以期为表皮角质形成细胞的长期培养和研究人类先天性眼睑发育缺陷机制提供参考。     

Recombinant hFGF-10 adenovirus promotes proliferation of kerotinocytes

AIM:To construct the recombinant adenoviral vector containing human fibroblast growth factor 10 (hFGF-10) gene, and to study the effect of the recombinant adenovirus on the proliferation of kerotinocytes. METHODS:HFGF-10 gene was amplified by PCR and ligated with shuttle vector pAdTrack-CMV to get the recombinant plasmid pAdTrack-CMV-hFGF-10, which was linearized with PmeI and transferred into Escherichia coli BJ5183 containing the adenoviral bone plasmid pAdEasy-1 for homologous recombination to obtain the recombinant adenoviral plasmid pAdEasy-hFGF-10. The recombinant adenoviral plasmid was then transfected into HEK-293 cell line to package and amplify the recombinant adenovirus. The expression of hFGF-10 in HaCat cells infected with the recombinant adenovirus was detected by Western blotting. The influence of the recombinant adenovirus on the proliferation of kerotinocytes was checked by MTT. RESULTS:The recombinant adenovirus containing hFGF-10 gene was successfully constructed, which effectively infected HaCat cells. The result of Western blotting showed that a protein in culture media of the infected HaCat cells reacted with hFGF-10 antibody. The recombinant adenovirus stimulated the proliferation of kerotinocytes. CONCLUSION:HaCat cells infected with the recombinant adenovirus expresses and secrets hFGF-10 protein, which promotes the proliferation of HaCat cells.     

Mechanism of AKT1 negatively regulating breast cancer cell migration

AIM: To investigate the molecular mechanism and downstream signaling pathway by which AKT1 inhibition regulates breast cancer cell migration. METHODS: RNA interference was used to knockdown the expression of AKT1. Western blot assay was performed to examine the expression of AKT1 total protein, β-catenin total protein and β-catenin nuclear protein. Immunofluorescence was used to examine the cellular localization of β-catenin. Transwell assay was used to investigate whether β-catenin nuclear accumulation as an alternative pathway was responsible for breast cancer metastasis induced by AKT1 inhibition. RESULTS: The total protein expression of AKT1 was decreased in MCF-7 and MDA-MB-231 cells treated with AKT1 siRNA. A significant increase in the protein expression of β-catenin was observed in MCF-7 cells and MDA-MB-231 cells treated with AKT1 siRNA. Immunofluorescence staining showed that MCF-7 cells and MDA-MB-231 cells displayed strong β-catenin staining in the cytoplasm and nucleus after knockdown of AKT1 expression. The ability of tumor cell migration increased dramatically after treated with AKT1 specific siRNA in the breast cancer MCF-7 cells and MDA-MB-231 cells in Transwell assay. XAV-939 reversed breast cancer cell migration induced by knockdown of AKT1 expression. CONCLUSION: β-catenin nuclear accumulation contributes to AKT1 inhibition-mediated breast cancer cell migration.     

基于GIS的丹东地区柞蚕农业气候区划

根据当地柞蚕实际生产与气象条件的关系,确定了柞蚕生产的农业气候区划指标.运用丹东地区1:250000地形数据和丹东及周边地区气象站30年的气候资料,建立区划指标小网格推算模型,应用GIS技术对丹东地区柞蚕生产进行气候区划,将该区划分为气候最适宜区、气候适宜区、气候次适宜区和气候不适宜区,为丹东柞蚕生产的合理布局和发展提供参考依据.     

利用I-69杨树伐根萌芽更新与植苗造林的效果

对３ ａ 生Ｉ６９ 杨树伐根萌芽更新与植苗造林的成活率和生长量的连续观测结果表明，在幼龄期，伐根萌芽更新比植苗造林用工少，成活率高，生长量大，而且可以节省大量的造林和抚育管护经费，经济效益显著。     

关于木兰属玉兰亚属分组问题的研究

简述了河南木兰属玉兰亚属树种资源及树种形变的特异性，探讨了该亚属的分组依据及标准，提出了合并望春玉兰组和木兰组为木兰组及成立朱砂玉兰组的依据，该依据得到了酶学和数量分类学的证实．