禽流感病毒H9亚型血凝抑制试验抗原国家标准品的研制

按照国家一级标准物质的要求,研制了禽流感病毒H9亚型(株)血凝抑制试验抗原国家标准品.对标准品的物理性状、无菌、效价、特异性、水分、真空度、均一性、稳定性等进行测定,各项指标均符合要求。经过8家单位对其协作标定,并经统计学分析,该批抗原血凝效价为460?80,最终定值为400。该禽流感病毒H9亚型(株)血凝抑制试验抗原可以作为国家标准品,批号为200901。     

基于MapGISK9平台的永久性基本农田建库研究——以南城县为例

基于MapGISK9平台,概述了南城县项目区的永久性基本农田划定建库过程,并针对数据库中存在的一些不确定性技术因素进行重点论述,对实际操作中的一些技术难点提出了相应的解决方案。     

Expression of p-p38 MAPK in partial cerebral tissues after hypoxic-ischemic brain damage in neonatal A2AR knockout mice

AIM： To study the expression of p-p38 MAPK in partial cerebral tissues after hypoxic-ischemic brain damage (HIBD) in the neonatal adenosine A2A receptor knockout (A2AR-/-) mice. METHODS：Base on the modified Rice method, the model of HIBD was established. The total 64 C57/BL6 neonatal mice (7 days old) of A2AR-/-(KO) and corresponding wild type (A2AR+/+, WT) were randomized into sham-operated group and model group. The mice in model group were divided into 3 subgroups： 1 d after HIBD, 3 d after HIBD and 7 d after HIBD (n=8 for each group). The cortex and hippocampal CA1 region were used as the study areas. The neuronal apoptosis was detected using TUNEL assay combined with Nissl staining. The expression of p-p38 MAPK and activated caspase-3 was determined by the method of immunohistochemistry. The KO mice and WT mice were also taken from sham-operated group (SKO and SWT, n=10) and model group (MKO and MWT, n=30) 1 d after HIBD to assess the early neurological behavior. RESULTS：The apoptotic neurons, activated caspase-3 and p-p38 MAPK increased after HIBD and peaked at 1 d after HIBD in the cortex and the hippocampal CA1 region. The apoptotic neurons and the expression of activated caspase-3 in KO mice were significantly higher than those in WT mice at the same time point after HIBD. The expression level of p-p38 MAPK in KO mice were significantly higher than that in WT mice at 1 d and 3 d after HIBD. The expression of activated caspase-3 was positively correlated with the expression of p-p38 MAPK in neonatal mice after HIBD (in the cortex：r=0.957, P<0.01; in the hippocampal CA1 region： r=0.939, P<0.01). CONCLUSION：p-p38 MAPK might be involved in the aggravated neuron apoptosis and brain damage induced by A2AR knockout after neonatal HIBO.     

Mild hypothermia protects against injury of rat hippocampal neurons induced by oxygen-glucose deprivation

AIM：To study the protective effect of mild hypothermia (31~32 °C) on rat hippocampal neurons against oxygen-glucose deprivation (OGD)-induced injury and its possible mechanisms. METHODS：An OGD experimental model of rat hippocampal neurons in vitro was established to simulate cerebral ischemic-hypoxic injury. The rat hippocampal neurons were randomly divided into 4 groups：control group, mild hypothermia group, OGD group and mild hypothermia+OGD group. The cell morphology was observed under light and electron microscopes. The neuronal apoptosis was detected by flow cytometry. The activity of caspase-3 in the cytoplasm was measured by colorimetry. RESULTS：The neuronal injury was apparent after OGD, with a great increase in apoptotic rate (P<0.01). Compared with OGD group, the morphology of neuronal injury in mild hypothermia+OGD group was attenuated, and the neuronal apoptotic rate and the activity of caspase-3 in the cytoplasm decreased. The activity of caspase-3 in the cytoplasm increased after OGD, and was positively correlated with the neuronal apoptotic rate (r=0.823, P<0.05). The activity of caspase-3 in the cytoplasm also increased after mild hypothermia and OGD, and was also positively correlated with the neuronal apoptotic rate (r=0.841, P<0.05). CONCLUSION：OGD can increase caspase-3 activity in the neuronal cytoplasm and induce neuronal apoptosis. Restraint on caspase-3 activity in the neuronal cytoplasm may be the mechanism by which mild hypothermia protects against neuronal injury induced by OGD.     

H7N9 禽流感疫情对东莞家禽经营环节公共卫生安全的影响及思考

从2013 年4 月份东莞市检出H7N9 禽流感病毒感染阳性样品,造成家禽产业巨大经济损失的事件中,引发对东莞家禽经营环节公共卫生安全的思考。对东莞市东城、常平和凤岗三大家禽批发市场和各镇街的农贸市场、超市家禽经营商铺的家禽销售和屠宰情况进行调查,分析当前东莞市家禽经营环节公共卫生安全存在的问题与风险,提出从8个方面加强家禽经营环节动物防疫监督和采取科学的应对措施,以保障公共卫生安全和家禽产业健康持续的发展及社会稳定。     

优质稻品种“甬优9号”示范表现及高产栽培技术

甬优9号在沙县夏茂镇西街村示范种植,表现群体整齐、株型适中、丰产性好、米质优.总结了甬优9号示范表现及配套高产栽培技术.     

籼粳杂交稻甬优9号在永安种植表现与主要栽培技术

甬优9号是浙江省宁波市农业科学研究院作物研究所和宁波市种子公司用甬粳2号A与K6093杂交配组育成的籼粳三系杂交稻品种,在永安种植表现群体整齐、株型适中、穗大粒多、后期转色好、米质优、分蘖中等、易感稻曲病。总结了2010---2012年甬优9号在永安种植表现及主要栽培技术。     

HVJ-E作为禽流感H9亚型灭活苗免疫佐剂的展望

近年来,禽流感已经成为各国动物疾病防控的重中之重,其中H9N2亚型虽然是一类低致病性禽流感病毒,但其感染范围广,发病迅速,可造成重大损失。现阶段,使用灭活苗是预防H9亚型禽流感的主要方法,虽然该类疫苗可以使免疫鸡在临床上不表现发病症状,但并不能阻止其感染后的排毒和对肌肉品质造成一定的影响。研究发现,HVJ-E具有双重免疫调节作用,有希望作为H9亚型灭活苗免疫佐剂应用于临床。     

Two cases of acute polyradiculoneuritis in dogs consuming a raw poultry diet

A 9-year-old female mixed-breed dog presented with ascending flaccid tetraparesis, and a 5-year-old castrated male Poodle dog presented with ventroflexion of neck, dysphonia, and hindlimb weakness, which progressed to acute ascending tetraparesis. Both dogs were fed raw poultry for over 9 and 5 years, respectively. Blood examination and other test results were normal or unrelated to the present case. Fecal polymerase chain reaction analysis in the Poodle dog was positive for Clostridium perfringens and Campylobacter jejuni. Tetraparesis improved with supportive care in both dogs. Human IV immunoglobulin was only administered to the Poodle dog, which showed a shorter recovery (12 days compared to 34 days in the mixed-breed dog). Both dogs returned to normal conditions eventually.     

H9N2亚型禽流感病毒A/Chicken/Shandong/LY1/2017株反向遗传操作系统的建立

为了构建H9N2亚型禽流感病毒A/Chicken/Shandong/LY1/2017的反向遗传操作平台,通过RT-PCR技术分别扩增该毒株的8个基因片段,并分别克隆至双向转录表达载体PHW2000中.8个质粒共转染293T/MDCK混合细胞,96 h后将细胞板反复冻融3次收获细胞悬液,将其接种10日龄SPF鸡胚,96 h后收集尿囊液并将拯救毒株命名为rH9N2,测定其HA效价为1:256.rH9N2在HA、MDT、EID50、TCID50以及病毒生长曲线、空斑等方面保持了与亲本毒株一致的生物学特性.本研究成功构建了A/Chicken/Shandong/LY1/2017的感染性克隆,为今后通过基因替换、定点突变技术研究H9N2亚型禽流感病毒致病性的分子机制奠定了基础.