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191.
AIM: To investigate the expression and roles of family with sequence similarity 3, member C (FAM3C) in oral squamous-cell carcinoma cells. METHODS: The mRNA and protein expression levels of FAM3C in dysplastic oral keratinocyte (DOK) and oral squamous-cell carcinoma WSU-HN6 cells were detected by RT-qPCR and Western blot. The WSU-HN6 cells were treated with siFAM3C or FAM3C antibody. After 24, 48 and 72 h, the viability of WSU-HN6 cells was measured by CCK-8 assay, and the activation of protein kinase B (Akt) was detected by Western blot. Adenovirus was used to mediate over-expression of FAM3C in the DOK cells. The DOK cell viability was measured by CCK-8 assay after adenovirus infection for 24, 48 and 72 h, and the activation of Akt was detected by Western blot. RESULTS: Compared with the DOK cells, the mRNA and protein levels of FAM3C were significantly increased in the WSU-HN6 cells (P<0.05). The viability of WSU-HN6 cells transfected with siFAM3C was significantly inhibited at 48 h and 72 h (P<0.05). siFAM3C treatment inhibited the activation of Akt (P<0.05). FAM3C antibody treatment also suppressed the viability of the WSU-HN6 cells at 48 h and 72 h and the activation of Akt (P<0.05). Over-expression of FAM3C in the DOK cells promoted the cell viability at 48 h and 72 h and activated Akt (P<0.05). CONCLUSION: FAM3C might promote oral squamous-cell carcinoma cell growth by activating Akt. 相似文献
192.
Autophagy is a transport pathway from the cytoplasm to the lysosome, which is a major intracellular degradation/recycling system ubiquitous in eukaryotic cells. Autophagy regulation has achieved some gratifying results in the treatment of glioma. It is currently an exciting field of clinical development. In chemotherapy or radiotherapy, autophagy-related drugs are currently used in vitro and in vivo for treating tumors with significant effects. Autophagy inducers and inhibitors may potentially block tumor formation and enhance the anti-cancer immune response. A more comprehensive understanding of the role of autophagy in different stages of glioma development may guide the development of new therapeutic strategies. 相似文献
193.
为了提高农机现代化和批量化生产效率,实现基于个性化需求农机设计和制造,提出了面向机加工的云技术农机制造服务候选资源的发现方法,并设计了农机虚拟制造的云计算平台。在农机部件云制造虚拟平台中,首先利用云存储技术存放大量的候选制造企业资源样本数据,然后利用智能分类算法KNN对候选资源进行分类,根据农机部件制造需求的客户对候选资源进行优选,最后得到最佳的候选资源。基于云计算和3D渲染技术,构建了面向工序级和零件级服务的仿真环境,并通过仿真实验,得到了优选后服务方的服务时间、费用和合格率,为现代农机的批量生产和个性化加工服务提供了新的解决方案。 相似文献
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Zhang Wen-yu Xu Jia-hui Zhang Chun-yu Tong Hui-li Li Shu-feng Yan Yun-qin 《东北农业大学学报(英文版)》2021,28(3):38-47
Myoblast differentiation is an essential process during skeletal muscle development. C2 C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro. Dehydrogenase/reductase(SDR family) member 3(DHRS3) is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol. Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation. However, the effect of DHRS3 on mouse muscle cell differentiation was unclear. The objective of current study was to determine if DHRS3 affected muscle cell differentiation, and if DHRS3 was involved in muscle regeneration. Protein expression was determined by western blot and immunofluorescence analysis. The activation and inhibition of DHRS3 increased and decreased C2 C12 myoblast differentiation respectively, which indicated that DHRS3 could affect C2 C12 myoblast differentiation. DHRS3 expression was significantly changed during muscle regeneration, with the regeneration of muscle injury, the expression of DHRS3 tended to increase first and then decrease. It suggested that DHRS3 might be involved in muscle regeneration. In summary, this study confirmed the involvement of DHRS3 in C2 C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development. 相似文献
196.
中子辐照及其与赤霉素复合处理水稻种子对水稻苗期生长的影响 总被引:4,自引:1,他引:3
以快中子脉冲堆对水稻种子进行中子辐照后用赤霉素(GA3)处理,对处理的种子发芽和幼苗生长情况进行研究。结果表明,红矮B和CB的致死注量为486×1010/cm2,绵恢2009为900×1010/cm2,明恢63和绵恢2095则为1350×1010/cm2;半致死注量:红矮B和CB为198~486×1010/cm2之间,绵恢2009约为486×1010/cm2,明恢63为629.48×1010/cm2,绵恢2095为774.69×1010/cm2。辐射敏感性表现为保持系>恢复系,即红矮B、CB>绵恢2009>明恢63>绵恢2095。赤霉素为有效的中子辐射防护剂,40和80mg/L是水稻中子辐照复合处理适宜的浓度。 相似文献
197.
以哈白兔为试验动物,利用同位素示踪法研究外源环核苷酸(CNT)对3H-标记的赖氨酸(3H-Lysine)在动物体内的分布、转运与代谢影响,其示踪动力学可用二室模型来描述:^Y(t)=983.6281e-0.021935t+1773.9999e-0.083932t-983.6281e-0.432590t-0773.9999e-0.050399t+300.2820。研究结果证明,皮下注射95h后,对照组3H-Lysine主要分布于肾脏、心脏、肝脏、脾脏、肌肉中;cGMP组中主要分布分布于膀胱、肌肉、肺脏、肠中;cAMP组主要分布于肌肉、胃、肝脏、心脏、生殖器中;cGMP+cAMP组中主要分布于肌肉、膀胱、生殖器、脂肪。同时,心脏、肝脏、脾脏、肾脏、肌肉、脑、膀胱、肠器官中,皮下注射环核苷酸后均有显著(p<0.05)或极显著(p<0.01)的变化,说明外源性CNT对赖氨酸在哈白兔血液中的代谢及在组织和器官中分布均有明显影响。 相似文献
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为从分子水平研究甘肃境内典型养殖模式下甘肃高山细毛羊、小尾寒羊、藏羊、蒙古羊和滩羊5个绵羊品种的PRKAG3基因多态性与屠宰性能和肉品质的相关性,探讨以PRKAG3为候选基因,提高绵羊屠宰性能和肉品质的可能性。采用PCR-SSCP技术对5个绵羊品种的PRKAG3基因外显子4,5和内含子4多态性进行检测,并就PRKAG3基因多态性与9月龄屠宰性能和肉品质进行相关性分析。结果表明,在PRKAG3基因2 689 bp处发生了AG的突变,位于第4内含子第23 bp处,表现出3种基因型,AA、AB和BB。纯合度(Ho)分别为0.67,0.69,0.76,0.69,0.59,杂合度(He)分别为0.333 3,0.306 1,0.244 9,0.307 7,0.408 2,有效等位基因(Ne)分别为1.62,1.66,1.69,1.48,1.79,多态信息含量(PIC)分别为0.31,0.32,0.33,0.27,0.34。除藏羊外其他4个绵羊品种均处于Hardy-Weinberg平衡状态。关联分析表明,5个绵羊品种PRKAG3基因此变异位点的3个基因型间失水率性状差异显著(P0.05);BB基因型个体的胴体重显著高于AA型(P0.05),滩羊为极显著(P0.01),BB基因型个体的眼肌面积显著大于AA型(P0.05)(藏羊除外)。因此说明PRKAG3基因可作为候选基因用于绵羊产肉性能和肉品质的分子标记辅助选择。 相似文献