2个桉树无性系微量元素叶片营养诊断初探

为了进一步提高桉树Eucalyptus产量,对桉树施微肥试验区的叶片进行了分析.采用叶片营养诊断临界值法探讨了2个桉树无性系的微量元素营养状况,初步获得了刚果12W5桉E.ABL 12W5和尾叶桉U6E. urophylla U6的部分微量元素营养的诊断指标,并进一步在中试区作诊断评价和检验.结果表明:桉树叶片的钼、锌、铜、锰的质量分数与一般植物的有差异.2个无性系的微量元素叶片营养诊断临界值,刚果12W5桉为硼27.90 mg·kg-1 ,锌45.00 mg·kg-1 ,铜1.08 mg·kg-1 ,铁288.00 mg·kg-1 ;尾叶桉U6为:硼13.50 mg·kg-1 ,锌10.80 mg·kg-1 ,钼0.15 mg·kg-1 ,锰216.00 mg·kg-1 ,铁117.00 mg·kg-1 ,其中桉树叶片养分诊断标准与微肥中试区的叶片分析数据相吻合的有这2种桉树叶片中的硼,尾叶桉中的钼,刚果桉中的铜;而中试区的桉树叶片锌、铁、锰的分析数据均未能与诊断标准相吻合,故这3种元素暂不适宜用临界值法诊断指导施肥.表6参22     

一株瑞士乳杆菌的分离鉴定及其产VB_(12)的影响因素研究

对市售的乳酸饮品的乳酸菌进行了分离纯化,得到菌株HYWZ-6,结合16S r RNA基因序列分析,确定为瑞士乳杆菌(Lactobacillus helveticus),并以温度、Na Cl、胆盐、p H及柠檬酸三钠作为影响因素,对其产VB_(12)进行研究,并利用酶联免疫分析法对该菌在不同因素条件下培养100 h后产VB_(12)的含量进行测定。结果表明,HYWZ-6菌株在不同条件下产VB_(12)的差异性较大,35℃VB_(12)的含量最高,25℃最低,35℃时含量比25℃时高出175.2%;Na Cl质量分数与VB_(12)的含量呈反比,Na Cl质量分数为1%时VB_(12)含量最高,7%时最低,相差224.4%;胆盐质量分数为0.1%时VB_(12)含量达到最高;p H为4时VB_(12)含量最高,在p H为7时VB_(12)含量最低,前者是后者的5.8倍;柠檬酸三钠质量分数为2.5%时VB_(12)的产量最高,比最低产量高出143.2%。     

Effects of DHRS3 in C2C12 Myoblast Differentiation and Mouse Skeletal Muscle Injury

Myoblast differentiation is an essential process during skeletal muscle development. C2 C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro. Dehydrogenase/reductase(SDR family) member 3(DHRS3) is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol. Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation. However, the effect of DHRS3 on mouse muscle cell differentiation was unclear. The objective of current study was to determine if DHRS3 affected muscle cell differentiation, and if DHRS3 was involved in muscle regeneration. Protein expression was determined by western blot and immunofluorescence analysis. The activation and inhibition of DHRS3 increased and decreased C2 C12 myoblast differentiation respectively, which indicated that DHRS3 could affect C2 C12 myoblast differentiation. DHRS3 expression was significantly changed during muscle regeneration, with the regeneration of muscle injury, the expression of DHRS3 tended to increase first and then decrease. It suggested that DHRS3 might be involved in muscle regeneration. In summary, this study confirmed the involvement of DHRS3 in C2 C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development.     

Effects of N-methyl-D, L-aspartate on secretion of growth hormone-releasing hormone from the boar hypothalamic-preoptic area and median eminence in vitro

N-methyl-D, L-aspartate (NMA) elicited secretion of growth hormone (GH)-releasing hormone from both the hypothalamic-preoptic area and the median eminence that were collected from boars. We suggest that the previously described increase in GH secretion that follows peripheral treatment of swine with NMA is attributable, at least in part, to NMA-stimulated secretion of GH-releasing hormone from the central nervous system.     

嗅鞘细胞对无血清培养诱导PC12细胞凋亡的作用

采用无血清培养诱导PC12细胞凋亡,研究嗅鞘细胞(Olfactory ensheathing cells,OECs)对去血清后诱导PC12细胞凋亡中的作用。通过MTT法检测细胞的活性,细胞形态学观察,以及结合流式细胞仪检测细胞的凋亡率,井对不同组细胞凋亡率进行比较。结果表明：①OECs培养上清对去血清后PC12细胞的存活有明显的促进作用;②从形态学上观察,OECs对去血清诱导PC12细胞凋亡有明显的抑制作用,使得细胞向死亡过渡受阻;②流式细胞对细胞周期分析,去血清诱导后的PC12细胞,大部分细胞滞留在G1期,细胞向S期过渡受阻,造成G1期细胞的堆积,它们所占比例分别为：73．6％,25．9％,0．5％;而OECs联合培养组所占比例：53％,42．3％,4．7％;OECs上清组：42．1％,52．2％,5．7％;正常有血清培养组：45,6％,41．4％,13％。凋亡细胞所占百分比分别为：60．3％,45,6％,37．4％,0．5％。     

Effect of uric acid on the maturation and the biological function of BMDCs

AIM: To investigate the effect of uric acid on the maturation and the biological function of murine bone-marrow derived dendritic cells (BMDCs) in vitro.METHODS: BMDCs were cultured with GM-CSF, IL-4, LPS and uric acid. Cell phenotypes were analyzed by flow cytometry. MTT was used to detect the effect of uric acid on the function of BMDCs in stimulating the proliferation of T cells. IL-12 released by BMDCs was also detected. RESULTS: BMDCs were cultured and identified. Uric acid at concentrations of 200 mg/L and 400 mg/L increased the expression of the molecules (CD11c, CD83, CD86, IA/IE) on BMDCs surface and the IL-12 level in the culture supernatants (P<0.05), promoted the proliferation of T cells at the T: DC rate 5∶1, 10∶1, 20∶1 (P<0.05). However, uric acid at concentration of 70 mg/L had no effect on above molecule expression (P>0.05), no effect on T cell proliferation with BMDCs (P>0.05) was observed. CONCLUSION: Uric acid promotes the differentiation, maturation, the expression of co-stimulatory molecules, the IL-12 production in BMDCs and enhances the ability of BMDCs to stimulate the proliferation of T cell in a specical dose range.     

Study on the immunological function of sodium butyrate-induced immature human monocyte-derived dendritic cells

AIM: To investigate the immunological function of sodium butyrate-induced immature dendritic cells in vitro.METHODS: The human monocyte-derived dendritic cells were induced in the presence of human granulocyte macrophage-colony stimulating factor(GM-CSF) and interleukin-4 (IL-4), combined with sodium butyrate. The immunological function of sodium butyrate-induced dendritic cells was detected by the FCM, endocytic activity, T cells stimulatory proliferation capacity, and interleukin-12 (IL-12) production.RESULTS: Sodium butyrate could down-regulate the major histocompatibility complex(MHC) class II and costimulatory molecules of dendritic cells, increase the endocytic activity, induce a stage of T-cell anergey, and inhibit the T helper cell type 1-skewing factor IL-12 production. CONCLUSION: Sodium butyrate inhibits the maturation of dendritic cells and induces production of immature dendritic cells, which may help to explore the machenism of its epigenitic modification.     

高香乌龙茶新品种鸿雁12号的选育

对高香乌龙茶新品种鸿雁12号的选育过程、多年品比和区试试验结果进行了介绍和分析。结果表明:鸿雁12号属灌木型,中叶早芽,无性系新品种,其生长势强,产量高,品比试验3年平均产量比对照福建水仙增产41.3%,在4个省份区试点产量表现均较好,分别比对照种黄棪增产2.22%、10.29%、29.64%和32.76%。适制高香乌龙茶和花香绿茶,品质优异。高香乌龙茶外形绿润,花香高浓持久,滋味浓滑爽口,汤色黄绿明亮,感官审评比福建水仙高8.1分,比黄棪高2.55～4.2分;花香绿茶滋味浓爽,香气高长,汤色叶底绿明亮,香气品质优于福鼎大白茶。该品种抗寒旱和抗虫能力强,易扦插,种植成活率高,适宜广东、湖南、广西、福建等省生产应用。     

草花育苗大棚定时灌溉系统研制

研究了采用DS12C887提供实时时钟、单片机AT89S51进行控制的草花育苗大棚灌溉系统.该控制系统采用模块化的设计,把时钟单元设计成了一个单独的模块,这样可以向任意多的定时单元提供实时时钟信号,所以控制系统有很好的扩展性,可以根据大棚的数目来扩展控制器的数目.通过现场调试,其运行满足实际要求.     

添加V_(B_(12))、V_E、V_C对无角道塞特绵羊精液冷冻效果的影响

探讨在无角道塞特绵羊冷冻精液稀释液中添加VB12、VE、VC对精子冷冻品质的影响。在无角道塞特绵羊冷冻精液稀释液中添加不同质量浓度的VB12、VE、VC制作细管冻精,解冻后观察精子的成活率和顶体完整率等指标。结果显示,VB12添加0.003 g/L时能够显著提高解冻后的精子活率(P0.05)和精子顶体完整率(P0.05);VE添加2.5 g/L时,能够极显著提高解冻后的精子活率(P0.01)和顶体完整率(P0.01);VC的添加4.4 g/L时能够显著提高解冻后的精子活率(P0.05),但对冷冻后精子的顶体完整率无影响。在稀释液中添加VB12、VE和VC能使解冻后精子品质得到提高。VE添加后能够极显著提高冷冻精子的顶体完整率,应该优先考虑添加。