淀粉接枝聚丙烯酸钠高吸水树脂的电子束法制备及结构表征

本文将电子束辐照技术和功能材料制备技术相结合,采用10 Me V电子束辐照法引发糊化的木薯淀粉与部分中和的丙烯酸单体进行接枝共聚反应,并采用N,N’-亚甲基双丙烯酰胺为交联剂,制备出一种淀粉接枝聚丙烯酸钠系高吸水树脂,并通过红外光谱(FTIR)和扫描电子显微镜(SEM)技术对木薯淀粉和共聚产物的官能团结构和表面形态进行验证。采用正交试验设计方法分析单体/淀粉比、辐照剂量、交联剂/单体比以及单体中和度对该反应接枝参数以及吸水性能的影响,4种因素的变化均可引起共聚产物的吸水能力变化,影响显著性次序为:单体中和度单体/淀粉比交联剂/单体比辐照剂量。获得最佳吸水性能的反应条件为:AA/St=3.5 g·g-1,MBA/AA=5 mmol·mol-1,辐照剂量D=6k Gy,中和度为70%,上述条件下获得共聚产物的吸水倍数为435 g·g-1。本文为采用电子束法制备高吸水材料提供了科学依据与技术支持。     

中浙优10号作烟后稻种植表现及主要栽培技术

引进三系中籼杂交稻中浙优10号在南平市延平区作烟后稻试种,综合性状表现好且高产稳产。针对中浙优10号品种特性和烟后茬的特点提出配套高产栽培技术。     

双亚10号亚麻新品种的选育

双亚10号是以(78—97×奥尔沙)×(85—1832×FR2)复合杂交育成的,具有高纤、优质、抗旱、抗倒伏和抗病优点。区域试验每公顷原茎、长纤维和种子产量分别为6068.7kg、975.1kg和741.9kg,分别比对照品种黑亚7号增产6.6%、24.5%和18.4%。生产试验每公顷原茎、长纤维和种子产量分别为5326.1kg、861.0kg和449.1kg,分别比对照品种黑亚11号增产8.5%、23.7%和10.9%。长麻率20.6%、全麻率29.9%,纤维强度26.7kg。     

Clenbuterol Affects the Expression of Messenger RNA for Interleukin 10 in Peripheral Leukocytes from Horses Challenged Intrabronchially with Lipopolysaccharides

On four occasions, four horses with heaves and four horses with small airway inflammatory diseases inhaled 0.9% saline based aerosol mixtures with or without lipopolysaccharides (LPS). Prior to the first saline and LPS inhalation, horses were untreated, while three and a half days prior to the third and forth inhalation horses had received 0.8 μ g/kg clenbuterol intravenously twice daily. The messenger RNA (mRNA) expression of tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10 and interferon- γ (IFN- γ) was investigated by RT-PCR, all of which were expressed in the white blood cells of samples collected. Inhalation of LPS only changed the cytokine expression profile of IL-10, IL-4 and TNF-α mRNA which were higher after challenge with LPS. However in those horses that were treated with clenbuterol the LPS-induced IL-10 mRNA expression was shown to be suppressed. Further changes in IL-4 and TNF-α were not significant. Thus the results of this study indicated that clenbuterol can modulate the expression of IL-10 mRNA in peripheral white blood cells in those horses with small airway diseases that have been exposed to LPS. van den Hoven, R., Duvigneau, J.C., Hartl, R.T. and Gemeiner, M., 2006. Clenbuterol affects the expression of messenger RNA for interleukin 10 in peripheral leukocytes from horses challenged intrabronchially with lipopolysaccharides. Veterinary Research Communications, 30(8), 921–928     

荒漠草原不同植物群落夏季土壤呼吸特征研究

采用动态密闭气室分析法测定了荒漠草原不同植物群落土壤呼吸速率的日动态并分析了土壤呼吸对温度变化的响应。结果表明:沙蒿(Artemisia desertorum)群落、赖草(Leymus secalinus)群落、甘草(Glycyrrhi-za uralensis)群落和冰草(Agropyron crisatum)群落4种植物群落土壤呼吸速率日变化表现为不对称的单峰曲线形式。冰草群落、赖草群落与沙蒿群落之间土壤呼吸速率存在显著差异,而沙蒿群落和甘草群落则无显著差异。采用指数方程拟合各群落土壤呼吸速率与0-20cm平均土壤温度的关系均显著相关,但不同植物群落间土壤基础呼吸不一样,其值大小依次为:冰草群落>甘草群落>赖草群落>沙蒿群落。Q10值的变化范围为1.22-14.75,且随着土壤深度的增加而增大,其平均值大小依次为:沙蒿群落>赖草群落>甘草群落>冰草群落。     

迪甜10号的选育及配套种植方式

迪甜10号是以自选自交系919为母本、737为父本杂交选育而成的超甜玉米杂交种,2011年通过山西省品种审定委员会审定(晋审玉2011024)。该品种于2008,2010年参加山西省区域试验,平均鲜果穗产量1.163万kg/hm2。2009—2010年参加生产试验,可进行2茬种植,第1茬平均鲜果穗产量1.280万kg/hm2,第2茬平均鲜果穗产量为9 690.0 kg/hm2,可提早、延后上市,经济效益显著。该品种生育期77.8 d,属于特早熟品种,提早栽培优势明显;籽粒黄白色,品质上等;抗病抗逆性较强,适应性广,高产稳产,适于积温2 300℃以上的地区种植。     

野猪CAPN10基因的生物信息学分析

为了研究CAPN10基因的性质和功能,使用NCBI等网站提供的一系列在线工具和软件,对其基因序列及其编码蛋白的结构和特性进行生物信息学分析。结果表明,野猪CAPN10基因CDS全长2 004 bp,编码667个氨基酸,同源性比对发现CAPN10保守性较低,对选择编码氨基酸的密码子具有偏好性;进一步的蛋白特性分析表明,野猪CAPN10是一种等电点为8.24的不稳定水溶性蛋白,无信号肽序列和跨膜结构,具有磷酸化等功能化位点及钙蛋白酶类催化基序结构。     

Wnt10b参与外源褪黑激素促进绒山羊绒毛生长的研究

本实验旨在研究Wnt10b在内蒙古成年绒山羊皮肤中的表达变化规律和褪黑激素(MT)对其表达量的影响。每隔1个月按照2 mg/kg BW的剂量在受试内蒙古成年绒山羊耳后皮下埋植MT。连续采集12个月(从2009年8月到2010年7月)的肩胛部皮肤样品,应用实时荧光定量PCR技术检测Wnt10b的表达量。结果表明:Wnt10b在内蒙古成年绒山羊皮肤组织中毛囊生长中期和休止前期高表达;埋植MT提高了Wnt10b在内蒙古成年绒山羊皮肤组织中毛囊休止期和退行期的表达量。结果提示,Wnt10b参与内蒙古绒山羊毛囊生长的信号传递过程,并在退行期和休止期转变过程中发挥作用;Wnt10b参与了外源MT促绒毛生长的毛囊周期性变化过程。     

Stem respiration is influenced by pruning and girdling in Pinus sylvestris

Abstract

Stem respiration was measured in the growing season (June to July) and in the dormant season (October) to detect cambial activity induced by pruning live branches or girdling stems in Scots pine trees (Pinus sylvestris L.) growing in northern Sweden. Immediately after the treatments, the treatment:control ratio of stem respiration increased to between 1.38 and 1.44 in the pruning treatment and between 1.17 and 1.20 in the girdling treatment. The treatment:control ratio of stem respiration then decreased by the end of July, to 0.65 in the pruning treatment and 0.55 in the girdling treatment. In October, the treatment:control ratios were higher: between 0.87 and 0.97 in the pruning treatment and between 0.85 and 0.97 in the girdling treatment. In both pruning and girdling treatments, the time trends of stem respiration rates largely followed those of stem temperatures: the stem respiration rate increased exponentially with an increase in stem temperature. The Q ₁₀ values were 2.83–4.05 and 2.57–2.89 in the pruning treatment and control, and 2.10–2.60 and 1.99–3.19 in the girdling treatment and control, respectively. In most cases, the values of Q ₁₀ in both treatments did not differ significantly from those in the controls.     

Iridoid, phenylethanoid and flavonoid glycosides from Sideritis trojana

From the MeOH extract of Sideritis trojana, a new iridoid glycoside, 10-O-(E)-feruloylmelittoside (1) was obtained in addition to four known iridoid glycosides [melittoside (2), 10-O-(E)-p-coumaroylmelittoside (3), stachysosides E (4) and G (5)]. Moreover, five phenylethanoid glycosides [verbascoside (6), isoacteoside (7), lamalboside (8), leonoside A (9), isolavandulifolioside (10), three flavone glycosides (isoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (11), 4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (12), 3'-hydroxy-4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (13) and a benzylalcohol derivative (di-O-methylcrenatin) were obtained and identified. The structures were elucidated on the basis of NMR and HRMS data. All compounds were tested for their antioxidant activity by in vitro TEAC assay and some of them exhibited moderate activity (0.97-1.44 mM) when compared with the reference compound (quercetin 1.86 mM). Glycosides 6-13, the most active compounds in the TEAC assay, were also tested by flow cytometry to evaluate their ability to affect the levels of reactive oxygen species (ROS) in human prostate cancer cells (PC3).