Effects of tetrandrine and fructose-1, 6-diphosphate on the elevated intrasynaptosomal [Ca2+]i induced by excitatory amino acids

AIM: To study the effects of tetrandrine(Tet) and fructose-1, 6-diphosphate(FDP) on the elevated intrasynaptosomal [Ca²⁺]_i induced by excitatory amino acids(EAA). METHODS: A rapid method for preparing synaptosomes was used, and intrasynaptosomal free calcium([Ca²⁺]_i) was measured by using the fluorescent indicator quin-2. RESULTS: L-glutamate(Glu, 100 μmol/L), aspartate(Asp, 100 μmol·L^-1), N-methy1-D-aspartate(100 μmol/L) and Glu(50 μmol/L) plus Asp(50 μmol/L) all elevated intrasynaptosomal [Ca²⁺]_i in a dose-dependent manner. Pretreatment with Tet(10, 30, 60 μmol/L), FDP(15, 30, 75, 150 μmol/L), MK-801(10, 20 μmol/L) and Tet(15, 30 μmol/L) plus FDP(15, 30 μmol/L) all attenuated the increase in intrasynaptosomal [Ca²⁺]_i induced by EAAs mentioned as above in a dose-dependent manner, and the effect of Tet plus FDP was most significant. CONCLUSION: Both Tet and FDP inhibited a rise in intrasynaptosomal [Ca²⁺]_i induced by EAAs, which may be one of mechanisms that Tet and FDP pretect cerebral tissues against ischemia injury.     

Effects of angiotensin II-induced hypertension chemotherapy on the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas

AIM: To investigate the activity of interleukin-1β converting enzyme in transplanted intracerebral rat gliomas under angiotensin II-induced hypertension chemotherapy. METHODS: The brain tumor model was produced in Wistar rats by stereotaxic inoculation of C6 glioma cells (1×10¹² /L). Tumor-bearing rats were treated with carmustine, teniposide and lisplatin (chemotherapy) during angiotensin II-induced hypertension. Then, the survival time of tumor-bearing rats, tumor blood flow, the concentration of drug, volume of gliomas and the activity of interleukin-1β converting enzyme in glioma were examined.RESULTS: The survival time of tumor-bearing rats was significantly longer in chemotherapy with angiotensin II-induced hypertension group than that of chemotherapy alone. In addition, regional tumor blood flow, the concentration of chemotherapeutic drug and the activity of interleukin -1β converting enzyme in transplanted rat gliomas were increased, while the volume of gliomas was decreased in hypertention chemotherapy group compared with chemotherapy alone. CONCLUSION: Chemotherapy with angiotensin II-induced hypertension has a enhancing effect on chemotherapy for improving the drug delivery to tumor tissue by a increased tumor blood flow and enhancing activity of interleukin -1β converting enzyme.     

两种复合肥在叶菜类蔬菜上的应用效果比较

在山东省莱阳和寿光两地露地及保护地生长的芹菜和大白菜上进行新型复合肥 2 1 8 11S肥效研究 ,同时与市场上较为流行的 15 15 15S复合肥进行比较。结果表明 ,2 1 8 11S复合肥比 15 15 15S复合肥在增加产量、提高品质方面效果更好。 2 1 8 11S复合肥最佳用量大白菜为 14 14～ 170 0kg·hm-2 ,芹菜为 2 0 6 2～ 2 4 78kg·hm-2 。     

西瓜新品种‘红冠龙’

 ‘红冠龙’为中熟西瓜一代杂种, 全生育期100 d 左右, 果实椭圆形, 平均单瓜质量8 kg 以上, 瓤红色, 可溶性固形物含量12. 2 %, 高抗枯萎病、炭疽病, 抗旱耐湿性均佳, 适合全国主要西瓜产区种植。     

Identification of Rhizoctonia solani AG 1-IB in Lettuce, AG 4 HG-I in Tomato and Melon, and AG 4 HG-III in Broccoli and Spinach, in Brazil

Fungi isolated in Brazil, from lettuce, broccoli, spinach, melon and tomato, were identified as Rhizoctonia solani. All lettuce isolates anastomosed with both AG 1-IA and IB subgroups and all isolates from broccoli, spinach, melon and tomato anastomosed with AG 4 subgroup HG-I, as well as with subgroups HG-II and HG-III. DNA sequence analyses of ribosomal internal transcribed spacers showed that isolates from lettuce were AG 1-IB, isolates from tomato and melon were AG 4 HG-I, and isolates from broccoli and spinach were AG 4 HG-III. The tomato isolates caused stem rot symptoms, the spinach, broccoli and melon isolates caused hypocotyl and root rot symptoms on the respective host plants and the lettuce isolates caused bottom rot. This is the first report on the occurrence in Brazil of R. solani AG 4 HG-I in tomato and melon, of AG 4 HG-III in broccoli and spinach and of AG 1-IB in lettuce.     

辣椒新品种亚华205的选育

亚华205辣椒是由母本2105和父本K6242配制的一代杂种。该品种中早熟，丰产，果实长牛角形，果长18－25cm，横径3．5cm,平均单果重45g，果皮黄色，果面光滑，果条顺直，竦味中等，肉厚，空腔小，耐贮运，商品性好，一般667m^2产量3500－4500kg，适于华南、吕南及华北地区露地丰产栽培。     

转基因水稻对稻瘟病的抗性研究

 采用苗期初筛、复筛、抗谱测定和田间自然诱发试验等不同鉴定方法,对经分子检测证明已整合有碱性几丁质酶基因和β-1,3-葡聚精酶基因的22个转化系的转基因水稻植株进行稻瘟病抗性鉴定研究,筛选出对稻瘟病的抗性比原种对照七丝软占有明显提高的一系列转基因水稻品系,其中表现高抗的有来自F4-9转化株系的7个品系。高抗材料的R7代品系,经室内抗谱测定及田间病圃试验结果,仍然表现高抗稻瘟病。本研究通过转基因技术,成功地将优质感病品种改良成高抗品系,研究结果证明了利用基因工程手段培育抗病水稻新品种是一个非常有希望的育种途径。     

茎瘤芥新品种涪杂1号的选育

涪杂1号茎瘤芥母本是胞质雄性不育系96118-3A,父本是优良的茎瘤芥自交系920154。该一代杂种从出苗至现蕾150～155 d(天),属中熟类型。瘤茎近圆球形,加工性能好,品质优,适于四川盆地茎瘤芥产区种植,一般每667 m2产量2 500—3 000 kg,最高可达4 000 kg。     

干椒新品种蓉椒6号的选育

自交系9804和9909杂交配制成杂交一代干椒，植株生长旺盛，座果力强，较抗病毒病和疫病，果实指形，纵长17～19cm，横径1．2cm，青熟果绿色，老熟果鲜红色，单果重8．3g，味辣，品质佳，中熟品种，从定植到采收55d(天)，一般每667m^2产1500～1900kg。