不同水平维生素A对意大利蜜蜂春繁阶段群势及幼虫抗氧化性的影响

【目的】研究日粮中添加不同水平维生素A对意大利蜜蜂春繁阶段群势和幼虫虫体抗氧化活性的影响。【方法】春繁开始时选用本地意大利蜜蜂25群（群内无花粉贮备）,随机分为5组,分别饲喂添加了不同水平维生素A的试验日粮,维生素A水平分别为0、5 000、10 000、15 000、20 000 IU•kg-1,并测定各组蜂群群势及幼虫抗氧化活性。【结果】春繁阶段饲粮中维生素A添加水平对意大利蜜蜂群势及取食量有显著影响。在试验期末,维生素A水平为10 000 IU•kg-1组群势显著高于对照组和其它试验组（P＜0.05）;饲喂维生素A水平为10 000和15 000 IU•kg-1饲料时5日龄幼虫虫体抗氧化活性（总抗氧化能力（T-AOC）、总超氧化物岐化酶（T-SOD）活性、过氧化氢酶（CAT）活性）显著高于对照组和其它试验组（P＜0.05）,丙二醛（MDA）含量显著低于对照组和其它试验组（P＜0.05）;除维生素A水平为15 000 IU•kg-1组外,10 000 IU•kg-1组工蜂初生重显著高于对照组和其它试验组（P＜0.05）。【结论】意大利蜜蜂春繁阶段适宜的维生素A添加水平（10 000—15 000 IU•kg-1）有利于蜂群群势的增长和抗氧化能力的提高。     

A/J小鼠感染猪链球菌2型后脾脏消减cDNA文库的构建及相关差异基因表达分析

为了分离与猪链球菌2型感染密切相关的基因,利用抑制性消减杂交(SSH)技术构建了8周龄A/J小鼠感染猪链球菌2型后的脾脏消减cDNA文库,并对其中169个阳性克隆进行测序,去除冗余的cDNA序列载体并聚类拼接后共获得110条差异表达序列标签(EST)。利用GenBank的BLAST软件分析比较核酸和蛋白质同源性,发现共有36个基因与这些EST具有高度的同源性,它们分别参与细胞成分、免疫、信号转导、凋亡、转录等重要功能。这些差异表达基因主要有ATP/GTP结合蛋白1(Agtpbp1)、天冬氨酸-谷氨酸-丙氨酸-天冬氨酸盒多肽5、核蛋白p68、ATP-依赖RNA解旋酶、真核生物转录因子2亚基(Eif2s2)、Na+/K+-ATP酶转运β3多肽、溶菌酶1、溶菌酶2等基因。结论:这些差异表达基因在猪链球菌2型感染过程中可能发挥重要功能。     

超级杂交稻Ⅱ优航1号高产栽培产量结构分析

【目的】揭示超级稻Ⅱ优航1号的高产结构特征。【方法】2005-2006年调查了75块Ⅱ优航1号不同产量水平的产量结构,进行相关分析和通径分析。【结果】结果表明,每穗粒数对产量的贡献为61.47%,每公顷穗数为25.59%,结实率为9.32%。每公顷穗数和每穗粒数对每公顷总粒数的贡献分别为35.48%和62.70%。每公顷总粒数对库容量的贡献为99.96%。库容量对稻谷产量的贡献89.05%,结实率的贡献为10.23%。【结论】说明Ⅱ优航1号超高产的关键是应培育足穗大穗,增加单位面积的总粒数,扩大库容量,并注重提高结实率。     

《中国近现代史纲要》教学效果问卷分析——以吉林农业科技学院为例

问卷分析表明,本校《中国近现代史纲要》教改比较成功,尤其是一周时事工作历深受学生喜欢。目前存在的问题集中表现在不能激发学生的学习兴趣,因此,提高教师素质,增强学生的主体地位是解决问题的有效措施。     

不同菱角壳提取物抑制肺癌A549细胞生长的研究

目的研究不同菱壳水和乙醇提取物的抗癌活性。方法采用Folin比色法测定不同品种菱壳提取物中多酚的含量,通过MTT法测定二角菱壳、四角菱壳的水提取物、醇提取物对肺癌A549细胞生长抑制作用。结果四角菱壳多酚含量高于二角菱壳,醇提物多酚含量高于水提物;二角菱壳醇提取物对肺癌A549细胞的抑制率高于水提物,差异具有统计学意义(P<0.01);四角菱壳水提物对抑制率高于二角菱壳水提物肺癌A549细胞的生长,差异具有统计学意义(P<0.01)。结论菱角壳提取物对肺癌A549细胞生长具有较强的抑制作用,且菱角壳的醇提取物优于水提取物,四菱角壳优于二角菱壳。     

Cytologically atypical anal sac adenocarcinoma in a dog

A 10-year-old intact female Shetland Sheepdog with tenesmus had a subcutaneous mass at the left ventral aspect of the anus. On cytologic examination, 2 types of cells were observed. Most of the cells were oval to polygonal and had elliptical or elongate nuclei and a moderate amount of pale to basophilic cytoplasm. The remaining cells had round to oval nuclei and pale to basophilic cytoplasm. Cells of both types were loosely adhered to each other and were arranged in rosette-like structures. Both neoplastic cell types had fine homogenous chromatin and either a small indistinct nucleolus or no visible nucleolus. Mild anisokaryosis and anisocytosis were observed. Histologically, the mass consists of glandular structures formed by cuboidal cells admixed with bundles of spindle cells. Eosinophilic PAS- and Alcian blue-positive secretory material was found in the center of some glandular structures. Both neoplastic cell types had positive staining with paradoxical concanavalin A and expressed cytokeratin, but not vimentin, S-100, α-smooth muscle actin, or desmin. Based on location and histologic and immunohistochemical features, the final diagnosis was adenocarcinoma of the apocrine gland of the anal sac, which should be included as a cytologic differential diagnosis when spindle cells and typical epithelial cells are observed in masses in the region of the anal sac of dogs.     

Annual Energy Consumption and Feasibility Assessment of Ground -Source Heat Pump in Residential Building in Fuzhou City

This paper describes the annual energy consumption and economic assessment of air-source heat pump(ASHP) and ground-source heat pump(GSHP),based on hourly building load of typical house in Fuzhou.Analysis results show that the energy consumption of GSHP is 15% less than that of ASHP through all year,where there is 13% less in summer and 20% less in winter.Although the seasonal energy efficiency ratio of GSHP in Fuzhou is higher than that of ASHP,it has no obvious effect on the energy efficiency as it is in the hot summer and cold winter zone.In comparison with ASHP,the GSHP is not cost-effective.     

强宿根抗旱甘蔗新品种云蔗03—194的选育

云蔗03—194,亲系：新台糖25×粤糖97-20,中大茎,中早熟,丰产、高糖,自然脱叶,宿根性好,抗旱性和抗倒性强。云蔗03—194在国家区试中蔗茎产量101．4t／hm2,甘蔗糖分14．40％,舍糖量14．68t／hm2;在云南临沧、德宏、保山、红河等主产蔗区旱地生产示范中蔗茎产量117．6t／hm2,甘蔗糖分15．23％,含糖量17．43t／hm2;该品种适应于云南、广西、广东、福建等蔗区肥力中等以上的蔗区种植,在燥热蔗区种植优势更为明显。     

甘蔗新品种闽糖86-2121在漳州蔗区表现及高产栽培技术

2年新植2年宿根区域试验和多年生产示范表明,闽糖86-2121是个萌芽率一般,分蘖率强,高产稳产、含糖量高、蔗糖分一般,中晚熟、宿根性强、抗旱抗病力强的甘蔗新品种,栽培上要适当增加下种量,及早追肥,宿根蔗宜早松蔸管理。     

“北耳南扩”的喜与忧

对应"南菇北移"的食用菌产业发展趋势,概述了黑木耳产业形成"北耳南扩"趋势的原因,介绍了"北耳南扩"之喜忧,并提出了应对措施。