水稻新品种"吉农大27"选育报告

"吉农大27"水稻新品种是以"吉86-11"为母本、"吉农大3"为父本进行有性杂交后经系谱法选育而成.2年区域试验和1年生产试验平均产量分别为8 497.5 kg/hm2和8 764.5 kg/hm2,分别比对照品种"吉玉粳"增产4.7%和9.0%.该品种干2008年1月通过吉林省农作物品种审定委员会审定,其主要特点是高产、抗病、优质,适宜吉林省中熟、中早熟稻作区种植,尤其适宜吉林、通化、白城地区种植.     

鸭疫里默氏杆菌50S L27转座子插入突变株的生物学特性分析

为了鉴定鸭疫里默氏杆菌中与卡那霉素抗性相关的基因,本研究采用构建Yb2株转座子随机突变库结合卡那霉素抗性平板来筛选相关抗性基因.先以Yb2株为受体菌,携带质粒pEP4351的大肠杆菌BW19851(pEP4351)为供体菌,构建转座子Tn4351随机突变库.用含红霉素和卡那菌素的TSA进行筛选,得到卡那霉素耐药性降低的...     

陕北佳县“7·27”特大暴雨中心区侵蚀灾害调查

针对2012年陕北省佳县"7·27"特大暴雨侵蚀灾害,以暴雨中心区(王家砭镇)受灾最严重的行政村为对象,调查了"7·27"特大暴雨中道路、河岸、河道、房屋建筑、坡耕地、退耕林地、退耕草地等的侵蚀灾害情况。选取不同恢复措施的典型坡面,量测了地表植被、地形、土壤结皮等状况和"7·27"暴雨侵蚀产生的土壤侵蚀量。重点调查了不同生态恢复措施与恢复效果在该次暴雨中的抗侵蚀作用。对"7·27"特大暴雨侵蚀灾害的原因进行了初步分析。结果表明,植被盖度、植被物种多样性、坡度以及土壤生物结皮对于暴雨侵蚀的防御具有重要影响。     

巴西橡胶树MADS-27基因的克隆与生物信息学分析

以巴西橡胶树花芽为材料,利用RT-PCR技术从总RNA中扩增获得1个MADS基因。cDNA长度1 063 bp,开放阅读框717 bp,编码239个氨基酸,命名为HbMADS-27。在HbMADS-27氨基酸序列中具有1个较强的跨膜区,二级结构分析结果表明其属于混合型蛋白。HbMADS-27在N端前34～50氨基酸区域内为较强的疏水区,但整条多肽链表现为亲水性。对20个MADS蛋白进行系统进化分析,结果发现,巴西橡胶树的HbMADS-27基因与蓖麻的MADS-27基因亲缘关系最近,而与拟南芥MADS-27基因亲缘关系相对较远。     

Effect of 27nt-miRNA on apoptosis of human umbilical vein endothelial cells induced by oxidized low-density lipoprotein

AIM To investigate the effect of 27nt-miRNA （27nt-miR） on apoptosis of human umbilical vein endothelial cells （HUVECs） induced by oxidized low-density lipoprotein （Ox-LDL） and its underlying mechanism. METHODS HUVECs were cultured in vitro and grouped as below： normal control group, Ox-LDL group, 27nt-miR+Ox-LDL group, anti-27nt-miR+Ox-LDL group and negative control+Ox-LDL group. The cells in Ox-LDL group were treated with Ox-LDL at 40 mg/L for 48 h, while those in normal control group were untreated but cultured normally. The cells in 27nt-miR+Ox-LDL group, anti-27nt-miR+Ox-LDL group and negative control+Ox-LDL group were transfected with their corresponding lentiviral vectors under the same procedure, followed by treatment with Ox-LDL at 40 mg/L for 48 h to induce apoptosis. The cell viability was measured by CCK-8 assay. The migration capacity was detected by scratch assay. The caspase-3 activity was measured by caspase-3 activity assay kit. The apoptotic rate was analyzed by Hoechst 33258 and flow cytometry. The mRNA and protein expression levels of Bcl-2, Bax and caspase-3 were determined by RT-qPCR and Western blot. RESUITS： Compared with negative control+Ox-LDL group, the cell viability and migration ability were significantly decreased by over-expression of 27nt-miR in the HUVECs （P<0.05）, while the activity of caspase-3 and apoptosis induced by Ox-LDL were significantly increased （P<0.05）. Furthermore, the mRNA and protein expression levels of Bax and caspase-3 were significantly up-regulated （P<0.05）, and the mRNA and protein expression level of Bcl-2 was down-regulated in 27nt-miR+Ox-LDL group （P<0.05）. Meanwhile, all the above indexes showed an opposite tendency in anti-27nt-miR+Ox-LDL group. CONCLUSION 27nt-miR promotes Ox-LDL-induced apoptosis and inhibits the viability and migration of HUVECs in vitro, possibly through regulating the expression of apoptotic/anti-apoptotic proteins such as Bax,caspase-3 and Bcl-2.     

氮肥运筹对钵苗机插水稻性状及产量的影响

[目的]研究不同氮肥运筹对钵苗机插水稻性状及产量的影响。[方法]以杂交籼稻Ⅱ优118和常规粳稻武运粳27为材料,研究不同基蘖肥和穗肥比例对水稻茎蘖动态、成穗率、产量及其构成因素的影响。[结果]基蘖肥:穗肥为7∶3的氮肥运筹方式有利于提高穗数,增加产量;Ⅱ优118以基蘖肥178.5 kg/hm~2、总用氮量255 kg/hm~2的处理的产量最高;武运粳27以基蘖肥252.0 kg/hm~2、总用氮量360 kg/hm~2的处理的群体产量最高。[结论]该研究为钵苗机插水稻的最佳氮肥运筹方式的推广提供理论基础。     

Molecular,serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China

Apple stem pitting virus (ASPV) is an important causal agent of pear diseases. Nowadays, the infection status and molecular characteristics of the virus in old pear trees have never been investigated. In this study, we provide the first complete genome sequence of an ASPV isolate LYC from an over 300-year-old tree of a local Pyrus bretschneideri cultivar ‘Chili’ specifically grown at Laiyang area in China. ASPV-LYC possesses a chimeric genome consisting of 9 273 nucleotides excluding a poly(A) tail at its 3’ end and harboring a recombination region in its open reading frame (ORF1) with Aurora-1 and KL9 identified as the major and minor parents. Western blot analysis with antisera against recombinant coat proteins (CPs) of three ASPV isolates from pear indicates that ASPV-LYC is serologically related to these ASPV isolates, but with differential activities. Further biological tests on indicator plants of Pyronia veitchii show that ASPV-LYC can induce serious leaf and stem symptoms as other ASPV isolates. The results provide an important information for understanding molecular evolution of ASPV and suggest a need to prevent dissemination of the isolate among pear trees.     

禽白血病/肉瘤病毒群特异性抗原p27的分离纯化及其抗体的制备

采用细胞培养法增殖禽白血病/肉瘤病毒(ALV/RSV)A亚型,然后对其进行超速离心浓缩及蔗糖密度梯度离心纯化病毒,再采用电泳分离与电洗脱收集相结合的方法纯化p27蛋白.经检测,提取的p27蛋白具有良好的抗原性与免疫原性.用该蛋白免疫家兔制备的兔抗p27抗体,经ELISA方法检测其效价可达1:6400以上.     

湖南省辣椒上首次发现象耳豆根结线虫

应用线虫比较形态学、同工酶技术和IGS-PCR技术,对采自湖南省永州地区辣椒上的根结线虫进行种类鉴定。结果表明,该病原线虫为象耳豆根结线虫(Meloidogyne enterolobii),这是首次在湖南地区发现象耳豆根结线虫。     

浙江省地方鸡种禽白血病毒抗原检测与部分分离株GP85基因序列分析

为了解浙江地区地方品种鸡中J亚群禽白血病病毒(subgroup J avian leukosis vrius,ALV-J)的感染情况与流行毒株的分子特征,对浙江省部分地方品种鸡体内ALV的p27抗原进行了检测,从疑似感染鸡群中检测分离到5株ALV-J,并对其GP85基因进行PCR分子克隆和序列分析。研究结果显示:在检测的19个地方鸡品系中,所有品系的鸡样本都呈现抗原阳性,其中101系的抗体阳性率最高,母鸡阳性率为40.48%,公鸡阳性率为16.54%;126系次之,母鸡阳性率为28.87%,公鸡阳性率为3.30%;171系抗体阳性率最低,其母鸡阳性率仅为4.08%,公鸡的感染率为0。为了解地方鸡群中ALV-J的遗传变异,对分离到的5株ALV-J的GP85基因进行了分子克隆和序列分析。结果表明,5个分离毒株的GP85基因大小均为924 bp,与预期一致;本研究样品序列之间的核苷酸同源性为90.8%～98.5%,与原型毒株HPRS103核苷酸相似性为90.3%～97.0%,与国内其他分离株的同源性为86.5%～99.0%。分离株与福建(FJ201308株)和广东(WF13株)分离株的同源性...