茶树体内铝形态及铝累积特性

设置不同Al³⁺浓度对青茶进行50 d处理,调查茶树铝含量和铝的化学配位形态。结果表明,茶树体内的铝大多以有机态或螯合态形式存在;茶树老叶具有高积累铝的特性,但以5 mmol L^-1铝处理时,运输到叶片的铝减少,积累于茶树根部的铝增多。利用²⁷Al NMR技术检测表明,茶树各器官中普遍存在Al₁₃的强烈共振吸收峰;在各器官中还出现–0.38×10^-6处和–0.17×10^-6处的微弱吸收峰,为目前尚未检出的铝络合物形式;在5 mmol L^-1 Al³⁺处理下,青茶老叶中含有更多的Al-复合物,包括Al-草酸盐(1∶2)、Al-草酸盐(1∶2)和Al-磷酸复合物,说明茶树体中的铝通过与其他物质形成络合物以降低铝的毒性。     

Inheritance of temperature-sensitive leaf rust resistance and adult plant stripe rust resistance in common wheat cultivar PBW343

Genetic analysis of common wheat cultivar PBW343 confirmed temperature-sensitive leaf rust resistance and adult plant stripe rust resistance. At low temperatures, PBW343 was resistant to P. triticina (Ptr) pathotype (pt.) 121R63-1, and at high temperature it was resistant to Ptr pt. 121R127. The low temperature resistance to pt. 121R63-1 was attributed to interaction between dominant and recessive genes. The dominant gene involved in low-temperature resistance to pt. 121R63-1 also conferred resistance to pt. 45R35. The high-temperature resistance to Ptr pt. 121R127 was governed by a different single partially dominant gene. Agra Local (a commonly used susceptible check) and IWP94 (a leaf rust differential used in India) are also resistant to pt. 121R127 at high temperatures. An allelism test indicated that PBW343 and IWP94 possessed a common gene for high temperature resistance to this pathotype. The adult plant stripe rust resistance against P. striiformis (Pst) was possibly conferred by one gene in addition to Yr27.     

Absence of p21 WAF1 and p27 Kip1 Gene Mutations in Various Feline Tumours

The coding regions of tumour suppressor and cell cycle regulatory genes p21 WAF1 and p27 Kip1 were investigated in 101 feline tumours of various types. No damaging mutations were present in the analysed areas of the genes     

14-3-3σ regulates p27 and inhibits Rat1-Akt cell proliferation

AIM: The present study was designed to investigate the effect of adenovirus-mediated 14-3-3σ on the proliferation of Rat1-Akt cells and to explore whether this effect is completed regulating p27.METHODS: The effect of Ad-14-3-3σ gene transfection on Rat1-Akt cell proliferation was observed by using 5-bromodeoxyuridine (BrdU).Then immunofluorescence and kinase assay were used to detect the effect of Ad-14-3-3σ on the phosphorylated level and intracellular location of p27.RESULTS: Ad-14-3-3σ-infected cells had fewer BrdU-positive cells (45%) than control (which was set at 100%).However,Ad-β-gal-infected cells had a high percentage of BrdU-positive cells (98%).14-3-3σ gene transfection downregulated phosphorylation level of p27 and decreased Akt-mediated intracellular location of p27.CONCLUSION: Transfection of 14-3-3σ gene suppresses the proliferation of Akt overexpession cell line Rat1-Akt.14-3-3σ decreases phosphorylation of p27 by downregulating Akt kinase,blocks Akt-mediated cytoplasm dislocation of p27,and inhibits proliferation of Rat1-Akt cells.     

SIVp27抗原双抗体夹心ELISA检测方法的研究

目的建立检测SIVp27抗原的双抗体夹心ELISA方法并进行初步应用。方法使用ProteinA亲和层析柱纯化腹水中的SIVp27单抗,用过碘酸钠法对单抗进行辣根过氧化物酶标记。经抗体配对实验确定包被抗体和检测抗体,再用棋盘滴定法确定抗体工作浓度后,探索构建检测SIVp27抗原的双抗体夹心ELISA法。应用建立的双抗体夹心ELISA法,对SIV模型猴血浆标本和SHIV模型猴病毒分离培养上清标本进行了检测,并将其结果与Coulter公司试剂盒检测结果进行比较。结果以稀释至20ug/mL的2E12为包被抗体、1:400稀释的酶标3G3为检测抗体为ELISA最优体系。模型猴血浆标本和病毒分离培养上清标本的检测结果表明自建体系可以用于SIVp27抗原的检测,特异性良好,灵敏度略低于Coulter公司试剂盒。结论检测SIVp27抗原的双抗体夹心ELISA方法初步建成,为研制有独立知识产权的检测试剂奠定了一定的基础。     

长角血蜱甘肃株肌钙蛋白基因P27/30的克隆和序列分析

参照GenBank中长角血蜱致病性Okayama株肌钙蛋白P27/30基因的核苷酸序列,设计合成一对引物,从本实验室保藏的干净长角血蜱卵中快速提取总RNA,通过RT-PCR扩增出607 bp的肌钙蛋白基因,将其克隆于PGEM-T-Easy载体,对其进行序列分析,并推导出氨基酸序列,将这一序列与国内外已发表的长角血蜱肌钙蛋白基因进行比较分析。结果表明本实验室保藏的长角血蜱甘肃株与上述已发表的Okayama株核苷酸序列同源性为92.1%,氨基酸同源性为86%。长角血蜱甘肃株与Okayama株、四川孤雌生殖株的发育有一定差异。经RT-PCR分析表明,该基因在长角血蜱的卵、幼蜱、若蜱和饥饿成蜱这几个阶段均有表达。     

Effect of low molecular weight lignin fragments including oxalic acid in alkaline-oxygen stage waste liquor on Al toxicity

Our previous study indicated the existence of some low molecular weight contaminants in separated fractions (F1 to F4) of alkaline-oxygen stage waste liquor by spectroscopic analysis. In the present study, the quantities of these compounds were determined by capillary electropheresis (CE). Substantial amounts of oxalate and acetate were found in F3. The complexes between Al and oxalate as well as F3 were characterized by ²⁷Al-nuclear magnetic resonance (NMR) spectroscopy, and the spectra of Al-oxalate complexes demonstrated the ability of oxalate to chelate Al and to produce different forms of complexes at varied molar ratios. Plant growth experiments in the presence of Al-oxalate complexes suggested that at a proper range, oxalate has a favorable effect on the detoxification of Al toxicity. It can be assumed that oxalate in F3 also plays an important role in the efficiency of removing Al toxicity. ²⁷Al-NMR was proved to be a useful method for the study of complexes between Al and organic compounds without disturbing their equilibrium conditions.     

胃癌组织中p27蛋白表达及预后的研究

目的:研究p27蛋白在胃癌组织的表达情况及其与胃癌生物学行为、预后的关系.方法:免疫组化SP法检测胃癌组织中p27蛋白的表达.结果:胃癌组织中27蛋白的表达阳性率为36.8%,无脉管侵犯病例p27表达高于有脉管侵犯病例,二者差异有显著性(P<0.05);p27低表达、有脉管侵犯、肿瘤直径≥4cm这3种因素与胃癌的不良预后有关.结论:p27蛋白表达为判断胃癌组织恶性程度及预后的有价值指标之一.