抗锈高产花生品种汕油27在育种中的应用

花生品种汕油27具有突出的丰产性与抗锈性、优良的生态株型与广泛的适应性，是珍珠豆型花生抗锈高产育种上可成功利用的中心亲本。我国南方利用汕油27作亲本材料已育成了30多个花生品种，其中通过国家和省级审定品种19个。这些衍生品种主要应用于南方春秋两熟花生区，推广面积较大的品种有汕油523、汕油71、粤油223、粤油79和湛油30等。     

SC27微生物土壤增肥剂在温室瓠瓜、丝瓜上的应用效果

SC27微生物土壤增肥剂由澳大利亚珀斯生物遗传实验室研制开发,其主要成分为固氮菌、硝化菌、磷细菌、钾细菌、纤维素降解菌等27种高活性的土壤微生物.该产品通过调节土壤微生物种群,达到改良土壤,改善作物生根环境,提高化肥利用率,促进作物生长发育的目的.近年来,SC27在美国、加拿大、新西兰等国得到广泛的应用,取得了明显效果.我国浙江、福建、湖北等省引进该产品后,在蔬菜、水果、茶叶、粮食等多种作物上开展了试验、示范及推广工作,已有多篇文献报道SC27在黄瓜、番茄、甜椒和茄子等露地蔬菜上的应用效果[1～4].本文通过研究SC27对温室中的瓠瓜(俗称葫芦)、丝瓜等蔬菜产量和品质的影响,为SC27在温室蔬菜生产中的推广应用提供科学依据.     

HSP27 as a biomarker for predicting skin irritation in human skin

AIM：To discover the novel endpoints for skin irritation responses by two-dimensional electrophoresis (2DE)-based proteomics. METHODS：2DE-based proteomics was used to analyze the protein expression in human skin exposed to sodium lauryl sulfate (SLS), 10-undecenoic acid (UA) and 3,3-dithiodipropionic acid (DA) following the test protocol of the European Centre for the Validation of Alternative Methods (ECVAM). The altered protein expression was validated by real-time RT-PCR and Western blotting. RESULTS：Heat-shock protein 27 (HSP27) was up-regulated most significantly among the 8 identified proteins, consistent with the results of Western blotting. CONCLUSION：HSP27 may represent a potential marker or additional endpoint for the hazard assessment of skin irritation caused by chemical products.     

SC27菌剂对福眼龙眼果实生长、产量和品质的影响

田间试验结果表明 :每年土壤兑水浇施澳州 SC2 7菌剂 4 0和 80 m L·株 -1可提高龙眼产量 1.1- 1.6t·hm-2 ,这与 SC2 7促进土壤养分有效化 ,提高植株叶片中 N、蛋白质、糖和淀粉含量以及提高龙眼座果数和果实膨大系数有关 ;SC2 7可以增加龙眼中 17种氨基酸 (尤其是人体必需氨基酸 )含量 ,以及果实中蛋白质、糖、可溶性固形物、VC、P、Ca、Fe、Zn等含量 ,改善果品品质     

Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection

Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1 were 0.35 × 10⁹ and 0.86 × 10⁹, respectively. To investigate the diagnostic abilities of the rapid kit using these antibodies, we performed several clinical studies. Assessment of analytical sensitivity revealed that the detection threshold of the rapid diagnostic test was 2 ng/mL for recombinant p27 and 12.5 × 10⁴ IU/mL for FeLV. When evaluating 252 cat sera samples, the kit was found to have a kappa value of 0.88 compared to polymerase chain reaction (PCR), indicating a significant correlation between data from the rapid diagnostic test and PCR. Sensitivity and specificity of the kit were 95.2% (20/21) and 98.5% (257/261), respectively. Our results demonstrated that the rapid diagnostic test would be a suitable diagnostic tool for the rapid detection of FeLV infection in cats.     

山核桃溃疡病的化学防治研究

对山核桃溃疡病的化学防治方法进行了研究。结果表明:早春使用27%“908”进行预防,4月初施药时应尽可能刮尽罹病组织后,使用27%“908”、硫酸铜等涂刷病斑,可以取得较好防治效果。     

巴西橡胶树MADS-27基因的克隆与生物信息学分析

以巴西橡胶树花芽为材料,利用RT—PCR技术从总RNA中扩增获得1个MADS基因。eDNA长度1063bp．开放阅读框717bp,编码239个氨基酸．命名为HbMADS-27。在HbMADS-27氨基酸序列中具有1个较强的跨膜区,二级结构分析结果表明其属于混合型蛋白。HbMADS-27在N端前34-50氨基酸区域内为较强的疏水区,但整条多肽链表现为亲水性。对20个MADS蛋白进行系统进化分析,结果发现,巴西橡胶树的HbMADS-27基因与蓖麻的MADS-27基因亲缘关系最近,而与拟南芥MADS-27基因亲缘关系相对较远。     

葡萄蔗糖转运蛋白基因VvSUC27的克隆及其植物表达载体的构建

本研究从赤霞珠(Vitis vinifera L.cv.Cabernet Sauvignon)葡萄果实中提取RNA,克隆得到葡萄蔗糖转运蛋白基因VvSUC27;从中蔬6号番茄子叶提取DNA,克隆得到番茄果实特异启动子基因E8.以实验室保存的pCAMBIA 1301为起始载体,分别构建了2个植物表达载体pCE8-SUC27vs和pCE8-SUC27.在这两个载体中,VvSUC27的表达均受番茄果实特异启动子E8的调控,pCE8-SUC27vs含有来自拟南芥葡萄糖转运蛋白基因AtVGT1的细胞液泡膜定位的信号肽编码序列和NOS终止子;pCE8-SUC27包含来自葡萄的蛋白质细胞质膜定位的信号肽编码序列和NOS终止子,在pCE8-SUC27vs质粒的基础上,构建了E8控制下的GUS植物表达载体pCE8-GUS.植物表达载体通过冻融法成功地转入到农杆菌EHA105菌株中.将包舍不同质粒的根癌农杆菌注射到番茄果实中进行了启动子功能的瞬时表达验证,获得了阳性GUS染色结果.研究为下一步葡萄蔗糖转运蛋白基因VvSUC27在果实中的特异表达分析奠定了基础.     

Sensitivity of different human colorectal tumor cell lines to commonly used chemotherapeutic drugs in vitro

AIM: To observe the antitumor effect of 5 commonly used chemotherapeutic drugs on 11 human colorectal cancer cell lines in vitro. METHODS: CCK-8 method was used to determine the growth inhibitory effects of 5 antitumor drugs, which were expressed as the half growth inhibitory concentration (IC50) and sensitivity index IC50/PPC (peak plasma concentration) on 11 human colorectal cancer cell lines. The expression variations of heat-shock protein 27 (HSP27) and HSP70 at protein levels in human colorectal tumor cell lines treated with different chemotherapeutic drugs were observed by Western blotting. RESULTS: All the 11 colorectal cancer cell lines were sensitive to 5-fluorouracil (5-FU) and oxaliplatin (OHP) without drug resistant. Five colorectal cancer cell lines were sensitive to mitomycin (MMC), while the other 6 cell lines were moderately sensitive. Ten colorectal cancer cell lines except SW1116 were sensitive to docetaxel (DXL), while SW1116 cells were resistant to DXL. Nine colorectal cancer cell lines except LS174T and SW1116 were moderately sensitive to irinotecan (IFL), and SW1116 cells were also resistant to IFL, while LS174T cells were sensitive to IFL. After treated with the antitumor drugs, HSP27 was up-regulated in HCT116 cells and SW480 cells, while the expression of HSP70 didnt change. CONCLUSION: LS174T cells are multidrug-sensitive, while SW1116 cells are multidrug-resistant. 5-FU and OHP are the wide-spectrum anti-colorectal cancer drugs. Determining the sensitivity to chemotherapeutic drugs and the expression level of HSP27 can improve the accuracy in drug selection.