国审小麦新品种周麦27在安徽省应用前景分析

为了促进国审小麦新品种周麦27在安徽省的推广应用,该文根据周麦27于2009-2012年在安徽省参加国家黄淮南片麦区冬水组的试验结果,对该品种的特征特性和生产表现进行了总结分析。结果表明,周麦27号的产量三要素协调、丰产潜力大,综合抗性较为全面,适宜在安徽省北部麦区推广种植。并提出了周麦27号高产栽培技术要点。     

Immunoregulatory effects of Taishan Pinus massoniana pollen polysaccharide on chicks co-infected with avian leukosis virus and Bordetella avium early in ovo

In recent years, co-infection of chicken embryos with immunosuppressive viruses and bacteria occurs with an annually increasing frequency. Consequently, studies on new and safe immunoregulators, especially plant polysaccharides, have become a popular topic in the poultry industry. In the present study, we selected 300 specific pathogen free embryonated eggs, which were injected with subgroup B avian leukosis virus (ALV-B) and Bordetella avium (B. avium) to establish an artificial co-infection model. The chicks that hatched from these co-infected embryonated eggs were treated with Taishan Pinus massoniana pollen polysaccharide (TPPPS). Results indicated that relevant indices in the co-infection group were significantly lower than that in B. avium-only group. Furthermore, pathogenicity of B. avium was exacerbated, with the chicks exhibiting decreased body weights. The TPPPS groups exhibited gradual improvements in immune function and developmental status. Therefore, in terms of improving immunologic function and production performance, TPPPS could be used as immunoregulator for immune responses.     

双低杂交油菜皖油27的特征特性和保优、高产栽培技术

依据安徽省油菜区域试验和生产试验数据,介绍杂交油菜新品种“皖油27”的产量、品质、抗性等特征特性及保优高产栽培技术,为推广应用提供指导。     

孕鼠DEHP暴露影响胎儿早期卵母细胞H3K27me3表达的研究

本文以胎鼠卵母细胞为研究对象,分析了妊娠母鼠孕期暴露邻苯二甲酸二(2-乙基)己酯(DEHP)对胎鼠卵母细胞早期发育过程中组蛋白甲基化修饰程度的影响,结果发现:妊娠母鼠在12.5 dpc到16.5 dpc 期间暴露40 μg/kg DEHP,第一次减数分裂前期的胎鼠雌性生殖细胞的H3K27me3表达受到了显著影响,导致H3K27me3强阳性细胞比例显著减少.该研究结果说明DEHP可通过孕鼠影响胎鼠卵母细胞早期发育过程中的组蛋白甲基化修饰.     

斑节对虾泛素融合蛋白UbS27基因克隆与表达分析

从构建的斑节对虾（Penaeus monodon）肝胰腺转录组数据中筛选出泛素核糖体融合蛋白UbS27基因（ubiquitin/ribosomal S27 fusion protein,PmUbS27）片段,利用SMART-RACE技术克隆出PmUbS27基因cDNA全长,并利用软件对其结构进行分析;利用实时荧光定量技术检测PmUbS27基因在斑节对虾不同组织及卵巢不同发育期的表达情况。结果显示,PmUbS27基因cDNA全长为514 bp,开放阅读框465 bp,编码154个氨基酸,含有泛素（1~72 aa）和核糖体蛋白（101~147 aa）2个结构域。以DNA为模板克隆得到的基因序列由3个内含子和4个外显子组成。实时荧光定量结果显示,PmUbS27基因在斑节对虾各组织中均有表达,但是在卵巢中表达量最高,其次为肝胰腺、血淋巴和精巢。PmUbS27在卵巢发育前期（Ⅱ期）和成熟期（Ⅴ期）的表达量显著高于其他各期（P0.05）。结果表明,PmUbS27参与斑节对虾卵巢发育的过程并可能在卵巢发育进程中起到重要的调控作用。     

森林草莓FveD27的表达特性和功能

[目的]新型植物激素独脚金内酯是调控植物分枝发育的关键因子,但独脚金内酯在草莓生长发育中的作用尚不清楚.揭示森林草莓(Fragaria vesca)中独脚金内酯生物合成关键基因DWARF27(D27)的表达特性及主要功能,探索FveD27在草莓分枝以及生长发育过程中的作用,为研究草莓植株构型奠定理论基础.[方法]本研究...     

Latent membrane protein 1 regulates expression of p27 and RPLP0 in nasopharyngeal epithelial and nasopharyngeal carcinoma tissues

AIM: To observe the protein expression of p27 and ribosomal phosphoprotein large P0(RPLP0) regulated by latent membrane protein 1 (LMP1) in nasopharyngeal epithelial and nasopharyngeal carcinoma tissues. METHODS: The protein levels of p27 and RPLP0 and the relationship with LMP1 were analyzed by Western blotting. The protein expression of LMP1, p27 and RPLP0 was also detected by the method of immunohistochemistry in 30 nasopharyngeal epithelial and 60 nasopharyngeal poorly-differentiated squamous-cell carcinoma tissues. Meanwhile, the significance of clinical pathology was evaluated. RESULTS: The positive rate of LMP1 protein was 73.3% and 90.0% in nasopharyngeal epithelial and nasopharyngeal poorly-differentiated squamous-cell carcinoma tissues, respectively. Compared with the LMP1-negative tissues, the protein levels of RPLP0 were low in the nasopharyngeal epithelial and nasopharyngeal poorly-differentiated squamous-cell carcinoma tissues with LMP1-positive expression, but the levels of RPLP0 protein were overexpressed. The protein expression of RPLP0 and RPLP0 was related to the age of nasopharyngeal carcinoma patients, the protein level of LMP1, the metastasis of lymph nodes and the TNM classification. The positive expression of p27 protein at high level was usually observed in the patients with young age, or had the characteristics of LMP1 (-), non-metastasis of lymph nodes, and in I or II stage of TNM classification. However, the protein expression of RPLP0 was low (P<0.05). CONCLUSION: LMP1 down-regulates p27 and up-regulates RPLP0 in nasopharyngeal epithelial and nasopharyngeal poorly-differentiated squamous-cell carcinoma tissues.     

鸡卵泡颗粒层组蛋白H3K4me3和H3K27me3的表达研究

卵母细胞成熟过程受组蛋白H3K4me3(trimethylation of lysine 4 on histone 3)和H3K27me3(trimethylation of lysine 27 on histone 3)及其相关的甲基化和去甲基化酶的调控，因此考虑对鸡的卵泡发育也存在一定的影响。选取“苏禽3号”配套系第一母本为研究对象，采用Western blot法探究组蛋白H3K4me3和H3K27me3在鸡卵泡不同发育阶段颗粒层中蛋白的表达模式。结果表明：在苏禽3号卵泡颗粒层中，组蛋白H3K4me3在卵泡发育不同阶段表达模式呈降低→升高→降低→升高的波浪形趋势，波浪变化较为平缓，在F5、F2和F1 3个表达高点的表达量与SWF(small white follicle)、LWF(large white follicle)、SYF(small yellow follicle)和F3 4个表达低点的表达差异显著（P<0.05）。组蛋白H3K27me3在不同发育阶段表达模式亦呈波浪形表达趋势，波浪变化起伏较明显，在SWF、SYF和F33个表达高点的表达量与F5、F4、F1和F2 4个表达低点的表达差异显著（P<0.05）。相关性分析显示，组蛋白H3K4me3与H3K27me3在不同发育阶段卵泡颗粒细胞中的表达呈较强的负线性相关(R=-0.808，P=0.000)。结果提示：组蛋白H3K4me3和H3K27me3在不同发育阶段卵泡颗粒层中的表达具有组织差异性，呈负相关的动态修饰性，可能共同协调卵泡生长过程中各基因的表达与功能，研究结果为鸡繁殖性状调控机理提供了理论依据。