排序方式: 共有10条查询结果,搜索用时 15 毫秒
1
1.
甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响 总被引:3,自引:0,他引:3
为研究甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响,分别用从甘蔗品种桂糖28号、新台糖22号、B8中分离获得的3种内生固氮菌及其混合液对甘蔗浸种处理,并测定与氮代谢相关酶活性指标。结果表明,接种甘蔗内生固氮菌对甘蔗叶片硝酸还原酶活性和谷氨酰胺合成酶活性具有普遍而明显的促进作用,而对谷草转氨酶与谷丙转氨酶的影响则因甘蔗品种差异表现出不同效果;巴西品种B8的谷草转氨酶与谷丙转氨酶活性显著提高,但固氮菌对本地品种桂糖28号和新台糖22号的谷草转氨酶与谷丙转氨酶活性影响不大。 相似文献
2.
从广东箣竹中分离、筛选得到5株固氮酶活性高且稳定、生长势强的内生固氮菌株,对其生理特性、环境适应性及其对植物的促生效果进行了系统研究.结果表明:在无氮培养、温度18~40 ℃时,菌株均能生长且有固氮酶活性,其最适生长及固氮的温度为26~37 ℃;在偏酸(pH值5.0)和偏碱(pH值8.0)的条件下,菌株均能保持较强的生长势和较高的固氮酶活性,并能通过调节自身代谢适应环境的酸、碱变化,使培养液趋近中性;培养液中NaCl浓度在0.5~2.5 g·L-1、(NH4)2SO4浓度在0.05~0.50 g·L-1时,菌株均能保持旺盛生长且有较高的固氮酶活性.接种水稻试验表明:菌株对植物的生长有一定的促进作用. 相似文献
3.
从已分离的多个狗牙根(Cynodon dactylon)内生固氮菌株中筛选到两株固氮酶活性较高的菌株7D和BM13,为明确固氮菌株对环境因子的适应性,研究了在不同的生长环境条件下菌株的生长量。采用Ashby无氮培养基,测定了温度、pH、渗透压以及不同氮源和碳源等环境条件对固氮菌生长的影响。结果表明,在10~40℃的温度范围内、pH值3.92~9.64的酸碱环境和30g·L~(-1) NaCl的高渗透压下,固氮菌7D和BM13均能正常生长;菌株能耐受0~10mmol·L~(-1)的NH_4~+和NO_3~-,而且能利用蔗糖、甘油、麦芽糖、甘露醇和葡萄糖等多种碳源生长。说明狗牙根内生固氮菌株7D和BM13对环境因子有很强的抗逆性和适应性,但两菌株之间存在一定差异,可望进一步研发成为优良的固氮微生物肥料生产菌种。 相似文献
4.
5.
6.
Gabriela C. ALVES Carlos L. R. DOS SANTOS Jerri E. ZILLI Fabio B. DOS REIS JUNIOR Ivanildo E. MARRIEL Farley A. da F. BREDA Robert M. BODDEY Veronica M. REIS 《土壤圈》2021,31(4):583-595
Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha~(-1) and was compared to the application of 40 and 80 kg N ha~(-1) without inoculation. Crop productivity and N accumulation in the grain were evaluated in addition to ~(15)N natural abundance(δ~(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha~(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha~(-1) and increased grain N content, especially in the off-season with 40 kg N ha~(-1). The inoculation treatments showed lower δ~(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season. 相似文献
7.
内生固氮菌HAUM10对水稻的侵染定殖规律及促生效应 总被引:1,自引:0,他引:1
试验以从表面灭菌的稗草中分离到的一株内生固氮菌HAUM10为对象,研究发现该菌具有较强的产固氮酶和吲哚乙酸能力.乙炔还原法测定其所产固氮酶的活性为0.824 μmol C2H4/(mL·h),液相色谱法测定其产吲哚乙酸量为15.44 μg/mL.将HAUM10用gfp基因标记后接种水稻,观察其在水稻中的侵染定殖规律,发现在接种后第7天,gfp标记的HAUM10主要定殖于根内通气组织和韧皮部细胞,少量定殖在叶鞘中.盆栽试验表明,HAUM10促进水稻叶绿素合成,有利于光合作用,在水稻生长后期促进氮、磷元素由叶向穗转移. 相似文献
8.
紫云英季土壤固氮微生物对外源碳氮投入的响应 总被引:3,自引:0,他引:3
【目的】分析不同外源有机物料(稻草、葡萄糖)及氮素投入对紫云英季土壤固氮微生物的调控作用,为我国南方紫云英-水稻轮作体系中秸秆还田及化肥减施增效提供支持。【方法】采用盆栽试验,共设7个处理,即CK(对照,不添加有机物料和氮)、稻草等量添加并配施不同量氮素(分别表示为Rs、RsN1和RsN2,对应C/N比分别为66、25和13);等秸秆碳量添加葡萄糖并配施不同量氮素(即Glc、GlcN1、GlcN2)、调整C/N比与秸秆添加相应处理保持一致。采集紫云英快速生长期土壤样品,利用Illumina Miseq PE300高通量测序和绝对定量PCR技术分析固氮功能基因nifH及固氮微生物群落特征。【结果】单独添加外源秸秆或葡萄糖处理的土壤C/N与对照无明显差异,但增施氮肥后C/N比呈下降趋势,GlcN2处理土壤C/N显著低于对照;对于土壤速效养分,Rs和RsN1处理土壤NO3--N含量与CK类似,但RsN2处理明显增加了60%;而添加葡萄糖处理土壤NO3--N含量整体较高(增幅为35%—79%);稻草单独添加或与氮素配施对土壤速效磷含量无明显影响,添加葡萄糖处理则显著降低其含量,降幅为16%—2... 相似文献
9.
Mirko Mutalipassi Gennaro Riccio Valerio Mazzella Christian Galasso Emanuele Somma Antonia Chiarore Donatella de Pascale Valerio Zupo 《Marine drugs》2021,19(4)
Cyanobacteria are a diversified phylum of nitrogen-fixing, photo-oxygenic bacteria able to colonize a wide array of environments. In addition to their fundamental role as diazotrophs, they produce a plethora of bioactive molecules, often as secondary metabolites, exhibiting various biological and ecological functions to be further investigated. Among all the identified species, cyanobacteria are capable to embrace symbiotic relationships in marine environments with organisms such as protozoans, macroalgae, seagrasses, and sponges, up to ascidians and other invertebrates. These symbioses have been demonstrated to dramatically change the cyanobacteria physiology, inducing the production of usually unexpressed bioactive molecules. Indeed, metabolic changes in cyanobacteria engaged in a symbiotic relationship are triggered by an exchange of infochemicals and activate silenced pathways. Drug discovery studies demonstrated that those molecules have interesting biotechnological perspectives. In this review, we explore the cyanobacterial symbioses in marine environments, considering them not only as diazotrophs but taking into consideration exchanges of infochemicals as well and emphasizing both the chemical ecology of relationship and the candidate biotechnological value for pharmaceutical and nutraceutical applications. 相似文献
10.
1