荔枝转基因抗性胚性愈伤组织的酯酶同工酶分析

通过对荔枝转基因抗性胚性愈伤组织(EC)的酯酶(EST)同工酶分析发现,9个转基因抗性EC的EST同工酶谱带没有差异,并且与对照的未转化HX30的细胞系差异也不大,这表明荔枝胚性愈伤组织的酯酶同工酶具有保守性,并未受到转基因的影响,可以作为不同荔枝品种转基因材料混杂鉴定的标记。     

Simulation of herbicide use in a crop rotation with transgenic herbicide-tolerant oilseed rape

The potential impact of herbicide-tolerant winter oilseed rape ( Brassica napus L.) on future herbicide use was investigated with a simulation model. The model uses a sigmoid function to simulate the growth of crops and weeds that compete for a maximum yield potential. Thresholds for weed control are based upon critical levels of weed biomass. The dynamics of the weed population are determined by the efficacy of representative herbicides on individual weed species and by seedbank parameters. Herbicide efficacy is determined by a log-logistic dose–response curve for each species. Simulation of a rotation with winter oilseed rape/wheat/wheat/barley showed contradictory predictions of herbicide use, because herbicide use in a rotation with either glyphosate- or glufosinate-tolerant oilseed rape was not reduced in the amount of kg a.i. ha^–1 compared with a traditional treatment, whereas the treatment frequency (number of standard recommended doses per unit area) decreased.     

转透明颤菌血红蛋白基因杨树的再生及其生长量初步测定

采用农杆菌介导法,将透明颤菌血红蛋白Vitreoscilla hemoglobin(VHb)基因vgb导入银腺杨(Populus alba ×P.glandulosa).以经无菌培养的叶片为外植体,通过450个叶盘与根癌农杆菌(Agrobacterium tumefaciens)LBA4404共培养,将植物双元表达载体pPZV中vgb基因导入银腺杨基因组,经卡那霉素筛选后,共获得60株卡那霉素抗性植株.经PCR特异性扩增和Southern点杂交分析,证明其中52株再生植株基因组DNA中整合了vgb基因.RT-PCR分析结果表明,vgb基因在其中的44个株系中获得表达.在2年温室生长条件下,转基因无性系的外部形态与对照相比无显著差异.但在株高和地径上,有3株转基因无性系较对照植株有明显增加.     

荔枝胚性愈伤组织基因枪转化系统的建立

荔枝是我国南方重要热带亚热带特产果树之一 .建立高效、稳定的荔枝转基因系统 ,对于应用现代生物技术进行荔枝的品种改良具有重要意义 [1] .我们在已建立的荔枝胚性愈伤组织 ( embryogenic callus,EC)体胚发生系统 [2 -4] 及其基因枪转化初步探讨 [5] 的基础上 ,进一步进行了 EC基因枪转化系统的建立研究 ,为将目的基因导入荔枝奠定了重要基础 ,并获得荔枝细胞工程研究中很有价值的有抗性筛选标记的荔枝胚性细胞系 .本研究中 ,以荔枝品种下番枝 (东刘 1号 )幼胚诱导的、长期继代保持的 EC为试验材料 ,建立了荔枝 EC高效遗传转化系统 :…