Structure and expression analysis of the sucrose synthase gene family in apple

Sucrose synthases (SUS) are a family of enzymes that play pivotal roles in carbon partitioning, sink strength and plant development. A total of 11 SUS genes have been identified in the genome of Malus domestica (MdSUSs), and phylogenetic analysis revealed that the MdSUS genes were divided into three groups, named as SUS I, SUS II and SUS III, respectively. The SUS I and SUS III groups included four homologs each, whereas the SUS II group contained three homologs. SUS genes in the same group showed similar structural characteristics, such as exon number, size and length distribution. After assessing four different tissues, MdSUS1s and MdSUS2.1 showed the highest expression in fruit, whereas MdSUS2.2/2.3 and MdSUS3s exhibit the highest expression in shoot tips. Most MdSUSs showed decreased expression during fruit development, similar to SUS enzyme activity, but both MdSUS2.1 and MdSUS1.4 displayed opposite expression profiles. These results suggest that different MdSUS genes might play distinct roles in the sink-source sugar cycle and sugar utilization in apple sink tissues.     

不同保存方法对角倍蚜性蚜数量和存活率的影响

比较了不同类型虫袋和填充物对角倍春迁蚜产性蚜数量和存活率的影响,结果表明:采用柱状虫袋填充玉米苞皮及对玉米苞皮进行浸泡处理可以提高性蚜的数量和雌蚜/干母的存活数;与传统的三角纸袋填充棕丝相比较,柱状虫袋填充浸泡玉米苞皮的雌蚜数、雌蚜/干母存活数、雄蚜数和性蚜总数分别增加了107.6%、229.1%、71.1%和87.5%,雌蚜/干母存活率提高了21.1%。虫袋内的雌蚜数、雄蚜数和性蚜总数与春迁蚜数呈显著负相关;雌蚜数和雌蚜/干母存活数与雄蚜数、性蚜总数和雌雄性比分别呈极显著正相关。角倍产区的环境因子明显影响春迁蚜的羽化期,但对春迁蚜产性蚜的数量没有明显影响。     

小麦与不同作物间作模式对麦蚜及主要捕食性天敌群落的影响

应用群落生态学结构变化特征的研究方法,研究了小麦与油菜、荷兰豆间作和单作麦田3种栽培模式条件下对麦田麦蚜及主要天敌群落的影响。结果表明,捕食性天敌的丰盛度和昆虫群落的稳定性,麦套荷兰豆最高,其次为麦套油菜田、单作麦田最低;麦套荷兰豆田麦蚜亚群落稳定性较好,单作麦田捕食性天敌亚群落的稳定性较好。麦蚜亚群落主要特征值分析表明,多样性指数和均匀度为:麦套荷兰豆>单作麦田>麦套油菜,优势度和优势集中性指数为:麦套油菜>单作麦田>麦套荷兰豆;主要捕食性天敌亚群落的多样性指数和均匀度为:单作麦田>麦套油菜>麦套荷兰豆,优势度和优势集中性指数为:麦套荷兰豆田>麦套油菜田>单作麦田。     

小麦穗蚜及其天敌的空间生态位分析

对合肥地区小麦穗期的三种蚜虫及其主要天敌的生态位宽度及重叠值进行了测定,分析了各种群在资源利用方面的种间关系。三种蚜虫蚜茧峰、瓢虫主要分布在麦株40cm以上的部位,每两种群之间的垂直生态位重叠值都达0.7以上;每种水平生态位宽度值都在0.6以上,每两种群之间水平生态位重叠值都在0.63以下。生态位随时间有一定的变化。麦蚜天敌的优势类群为蚜茧峰。     

Pollen and pollination experiments. I. the contribution of stray pollen to the seed set of depetalled,hand-pollinated flowers of apple

Summary Using the finger nails to remove most of the petals is a fast method of preparing apple flowers for hand pollination in the balloon stage. Such flowers still appeared quite attractive to insects; left to open pollination, the seed set per flower averaged two thirds of that of similarly treated, but hand-pollinated flowers. With the aid of marker pollen of scab or mildew resistant donors and the subsequent screening of the seedling progenies for resistance, it was shown that the stray pollen was responsible on average for one of every three seeds formed by unbagged depetalled and hand-pollinated flowers.     

苹果可溶性固形物含量的检测方法—基于可见光近红外光谱技术

为了快速检测苹果的可溶性固形物(SSC)含量,采用可见光近红外光谱技术,结合主成分分析(PCA)和BP神经网络技术,来建立苹果SSC的预测模型.获取苹果样本在345～1039 nm波段的漫反射光谱,采用DPS数据处理系统对其进行主成分分析,并提取出累计可信度大于95 %的5个新主成分.建立一个3层的BP神经网络模型,并将这5个新的主成分作为BP神经网络模型的输入量,其结果是98 %以上预测样本的预测相对误差在5 %以下.该研究表明,采用近红外光谱技术来建立苹果可溶性固形物的预测模型是可行的.     

几种苹果矮化砧木的抗寒性研究

以71-3-150、GM256、SH6及M9 4种苹果(Malus)矮化砧木的1年生枝条为试材,进行-20℃、-25℃、-30℃、-35℃、-40℃低温处理,用电导法配合Logistic方程,求得拐点温度(LT50)即半致死温度,并对其电导率、可溶性蛋白含量和POD酶活性等指标进行了测定比较。结果表明:测得的电解质渗出率呈典型的S型曲线,与相应的低温呈极显著负相关;71-3-150、GM256、SH6及M9的半致死温度分别为-40.63℃、-39.29℃、-34.10℃、-30.10℃;抗寒性强的71-3-150和GM256能保持较高的可溶性蛋白含量和POD酶活性。     

晋中盆地麦蚜发生为害程度及原因分析

晋中盆地麦蚜发生为害高峰常出现于小麦孕穗到灌浆期,大发生年发生高峰可提前至拔节期。从1998～2003年连续6年的系统观察调查得出,遇暖冬及早春温暖干旱气候,麦蚜呈现早发生或大发生;如遇冬春低温多雨,尤且是1～2月份极端温度低于-20℃,时间长达15d时,对麦蚜发生形成明显抑制。另外,蚜虫发生程度与天敌数量关系密切,小麦播种面积与蚜虫发生总量呈正相关关系。     

采后照光处理对富士苹果增色及贮藏的影响

采后人工照光使富士苹果增色的试验结果表明：白色荧光灯照射后果实花青素形成量最多;８℃和 ２０℃下果实花青素形成最快,２℃下最慢,但形成量最多;８０ Ｗ照射果实花青素形成量大于４０ Ｗ照射;２℃和 ８℃下采用白色荧光灯分别照射 １２０、１９２ｈ,可使果实照光面 １００％着色。对果实随后的低温贮藏无显著不良影响。     

Resistance to bird cherry-oat aphid (Rhopalosiphum padi L.) in winter wheat varieties

Summary In Hungary the bird cherry-oat aphid (Rhopalosiphum padi L.) is the most frequent aphid species in winter wheat (Triticum aestivum L.). Estimations of infestation by R. padi as well as measurements of grain yield and thousand-kernel mass were carried out in 26 winter wheat genotypes in conditions of naturally infested and not infested (protected) control plots. The experiment was performed in isolated conditions in two field cages covered by nets. The aphids overwintered on wheat and got into cage, extremely quickly multiplied, therefore there was no need to apply any artificial aphid infestation. Highly significant differences were demonstrated among genotypes in infestation severity of R. padi as well as in losses of grain yield and thousand-kernel mass. The most resistant variety GK Zombor had 25% infestation, and the most susceptible one GK Lili had 79.2%. The reduction of grain yield of the most tolerant genotypes (GK Korány, Downy, Mv 4, Jubilejnaja 50, Mv 8, GK Kincsö and GK Zombor) was 26–33%, and that of thousand-kernel mass was 23–30%. The most sensitive genotypes (GK Lili, GK Örzse, GK Koppány and Mv 13) suffered 58–63% losses in yield, and 40–50% in thousand-kernel mass. A close correlation was found between infestation of R. padi in different wheat genotypes and losses of grain yield (r=0.7572, P<0.001). Also there were tolerance differences among genotypes even within the same level of infestation. The reductions of thousand-kernel mass correlated very closely with the reductions of grain yield (r=0.9212, P<0.001), that makes screening possible by reductions of thousand-kernel mass. These results have found application in breeding. The leaf pubescence of the varieties studied did not generally influence the infestation by R. padi.