浅议伏牛山野生藤蔓植物的应用

结合垂直绿化的实际情况,讨论了伏牛山野生藤蔓植物在景观表现中的独特作用,对野生藤蔓植物的开发利用方式做了介绍。     

野生动物数量调查方法综述

从论述野生动物数量调查的目的意义开始,对国内外常用的野生动物数量调查的主要方法和发展趋势进行了综合性评述。     

伊犁河谷14种野生观赏植物种子活力及休眠破除研究

研究伊犁河谷14种野生观赏植物种子特性,探究其种子保存方法及休眠破除的最佳方案。结果表明,1)低温干藏和超低温干藏处理后,所有种子的活力均有不同程度的下降;2)顶冰花、喜盐鸢尾的种子休眠破除均对赤霉素响应不敏感;马兰、天仙子、菥蓂、团扇荠、花葵、锦葵和欧夏至草在0.1mmol/L赤霉素处理时萌发率最高,而酸模、毒芹、白芷和夏枯草在1mmol/L赤霉素处理下萌发率最高;3)低温干藏和超低温干藏后,顶冰花、白喉乌头、喜盐鸢尾种子休眠对其不响应;低温干藏后,马兰、酸模、花葵、团扇荠、天仙子种子萌发率较高,而超低温干藏后,菥蓂、锦葵、毒芹、白芷、欧夏至草和夏枯草种子萌发率较高。     

猪流行性腹泻病毒灭活疫苗的制备及攻毒保护研究

为研究现用猪流行性腹泻病毒(PEDV)CV777疫苗毒株对流行毒株的免疫保护效果,以RTPCR进行PEDV CV777疫苗株、JS12流行株S基因克隆测序,进行PEDV S基因核酸序列分析与氨基酸序列分析。分别以转瓶工艺与细胞悬浮培养工艺培养Vero E6细胞,制备PEDV CV777株转瓶灭活苗与悬浮灭活苗。分别以PEDV转瓶灭活苗、悬浮灭活苗、PEDV流行株组织灭活苗(JS12株)产前30d免疫经产母猪;母猪产仔后14d进行仔猪攻毒保护试验。核酸序列分析显示,PEDV CV777株S基因与流行毒株的相似性在96.4%~99.7%之间,氨基酸序列相似性在96.7%~99.5%之间。转瓶工艺制备PEDV抗原效价为107.0 TCID50/mL,悬浮培养制备PEDV抗原效价为108.5 TCID50/mL。攻毒保护试验显示,悬浮灭活苗免疫母猪后,仔猪有2/10发病,保护率为8/10,转瓶灭活苗组发病率为8/10,保护率仅为2/10;组织灭活苗组9/10发病;对照组10头全部发病。该试验证明,提高PEDV CV777株的抗原含量,能够有效提高疫苗对PEDV流行毒株的保护效果。     

不同栽培基质对毛木耳菌丝体生长的影响

[目的]研究不同栽培基质对毛木耳菌丝体生长的影响。[方法]按不同树种(油桐)、木屑粒径、有无淋洗以及是否添加杏鲍菇菌渣,设计14个处理,观察毛木耳菌丝体生长情况。[结果]尾巨桉粗木屑耳袋菌丝密度大、长势好,前期生长速度快、中后期略显后劲不足;木屑不淋洗处理菌丝的生长速度总体较快,前期生长速度慢、中期快、后期明显减慢,与淋洗处理相反;尾巨桉木屑添加杏鲍菇菌渣后,生长速度总体较慢,生长后期后劲不足;油桐木屑菌丝生长总体优于尾巨桉。[结论]该研究可为毛木耳栽培基质的筛选提供参考。     

Some Protozoan and Metazoan Parasites of the Amberjack From the Balearic Sea (Western Mediterranean)

A survey of protozoan and metazoan parasites of wild amberjack (Seriola dumerili) from the Balearic Sea revealed one myxozoan (Myxobolus sp.), one monogenean (Heteraxine heterocerca), 10 trematodes (Bucephalus polymorphus, Prosorhynchus crucibulum, Bucephalopsis sp., Hemiurus communis, Aponurus sp., Acanthocolpus Iiodorus, Stephanozostomum pristis, Nematobothrium scombri, Wedlia bipartita, Paradeontacylix sp.), one nematode (Philometra globiceps), one copepod (Caligus curtus) and one isopod (Gnathia vorax larva) species to be present. The prevalence and intensity of infection and the distribution of each species is described. Their possible interactions with farmed amberjack are discussed.     

海南特种野猪肌肉组织特性与胴体肉质性状的相关性研究

为了研究海南特种野猪肌肉组织学特性及其与胴体肉质性状之间的关系,试验测定了海南特种野猪骨骼肌不同部位肌纤维形态和胴体肉质性状指标,并进行了相关分析。结果表明,海南特种野猪肌纤维直径较小,肌纤维密度较大,肌纤维面积适中;肌纤维等效直径、面积、密度均与剪切力呈显著(P0.05)正相关;肌纤维等效直径和面积与肌内脂肪呈显著(P0.05)负相关;肌纤维长径与肉色和大理石纹均呈显著(P0.05)正相关。结果揭示了海南特种野猪肌纤维等效直径、面积、密度与肉质性状存在显著相关关系。其肌纤维细,肌纤维密度高,肌肉表面纹理细腻致密,嫩度好,肉质优良。     

云南野生稻新害虫食禾梯管蚜的空间分布型

 对食禾梯管蚜在2种野生稻上分布调查表明,该虫在2种野生稻上的空间分布图式是均为聚集分布,分布的基本成分为个体群。并得该害虫在2种野生稻上的最适抽样数模型,即:药用野生稻N=（11.968 8/m＋0.869 8）/D²,元江普通野生稻N=（2.804 3/m+0.618 3）/D²,景洪普通野生稻N=（2.456 6/m+1.187 3）/D²;并根据最适抽样数公式确定既定精度抽样停止线。     

Role of effective microorganisms in efficacy of Nemarioc-AG phytonematicide on suppression of Meloidogyne incognita and growth of tomato plants

The release of active ingredient chemicals in conventional organic amendments is primarily through microbial degradation, whereas in granular phytonematicides, leaching through irrigation water had been implied as a probable mechanism. The objective of this study was to determine the role of effective microorganisms (EM) on the efficacy of Nemarioc-AG (NAG; G?=?granular formulation) phytonematicide on suppression of root-knot (Meloidogyne species) nematodes and growth of tomato (Solanum lycopersicum) plants. A 5?×?6 factorial experiment, with EM at 0%, 0.14%, 0.17%, 0.20% and 0.25% and phytonematicide at 0, 3, 6, 9, 12 and 15?g per plant, was conducted under microplot conditions. Each plant was inoculated with 11,000 eggs and second-stage juveniles (J2) of Meloidogyne incognita. Seventy days after initiating the treatments, the interactions were not significant (P?≥?.05) on all variables, but the main factors were highly significant. Regression of nematode population densities, root gall and dry shoot mass over increasing phytonematicide concentration exhibited quadratic relations, which were explained by 93%, 97% and 99%, respectively. In contrast, stem diameter and phytonematicide concentration had a positive linear relation, which was explained by 98%. Nematode numbers over increasing EM concentration exhibited quadratic relation, which was explained by 97%. All plant variables and EM had no significant relations. In conclusion, the efficacy of NAG phytonematicide on nematode suppression and plant growth was independent of EM activities, suggesting that irrigation water played a major role in the efficacy of the product in granular formulation.