Reproductive barriers to hybridizations between narrow-leaf and broad-leaf Anoectochilus roxburghii

Narrow-leaf and broad-leaf Anoectochilus roxburghii were used as reciprocal parents to explore the reason for low seed setting rate. Pollen-pistil interaction was observed by fluorescence microscopy and hybrid embryo development by paraffin section technology. The results indicated that pollen tube growth could reach the embryo sac, and double fertilization could be completed. These findings showed that a pre-fertilization barrier was not the major factor in the cross of narrow-leaf and broad-leaf A. roxburghii. The endosperm development of the hybrid was abnormal and eventually promoted abortion of the embryos. A post-fertilization barrier appears to be the major factor for low seed setting rate in the cross of narrow-leaf and broad-leaf A. roxburghii. In addition, an efficient protocol of embryo rescue, the development of which is reported elsewhere, was used to compare the time needed for rescue between the reciprocal crosses.     

坎儿井衰减与地下水补排系统响应关系

为了探究坎儿井衰减与地下水补排系统响应关系,利用地下水位观测资料和坎儿井流量监测资料,结合鄯善县历次地下水资源调查评价资料和坎儿井统计数据,分析了地下水补给量、排泄量对坎儿井衰减的影响,并建立了最小二乘回归模型,在执行最严格水资源管理制度下对2020年、2030年坎儿井出水量进行了预测。结果表明,坎儿井流量动态与地下水位动态基本同步;北盆地的坎儿井出水量主要受地下水补给量的影响,南盆地主要受地下水开采量的影响。地下水开采量是影响鄯善县坎儿井衰减的关键因素,在最严格水资源管理制度下鄯善县坎儿井出水量将会在2020年恢复至0.83亿m~3,2030年恢复至0.87亿m~3。     

基于SWOT-AHP分析的内蒙古农牧业可持续发展研究

摘要：通过运用SWOT分析法，从多个维度确定影响内蒙古农牧业发展的因素，再运用AHP分析法，将SWOT分析的影响因素作为指标层，邀请专家打分构造判断矩阵，将其量化的指标按其重要性进行排序。通过计算总优势、劣势、机遇、威胁力度，绘制战略四边形，并且计算战略四边形重心，判断战略定位，最后进行战略选择。结果显示内蒙古农牧业可持续发展中机遇>优势>劣势>威胁。战略中心位于第一象限内，说明应该采取开拓性战略，战略方位角=49.14°，说明目前处于机遇期。因此内蒙古农牧业应该制定积极的发展战略，依靠内部优势，充分利用外部机会。     

绵羊卵泡发育过程中关键信号通路研究进展

卵泡从原始卵泡发育为成熟卵泡，直至排卵、黄体发育等过程都受到精密的调控，产生大量的优势卵泡是绵羊产多羔及实现快速扩繁的关键因素。研究发现，相关信号通路和转录因子通过影响绵羊卵泡中卵母细胞、颗粒细胞的生长，进而调控卵泡的发育成熟，对这些信号通路进行深入了解，有助于探索卵泡发育的调控机制，早日实现绵羊高效繁育。Notch是卵泡发育过程中发挥重要作用的高度保守信号通路，PI3K/AKT/mTOR信号通路各成员都是广泛存在于细胞内的信号转导分子，在卵泡发育早期发挥了主要作用，还有间隙连接（gap junction，GJ）和跨带突触（transzonal projections，TZPs）等物理连接方式，在细胞间的交流通讯起到重要作用。作者详细介绍了Notch信号通路、PI3K/AKT/mTOR信号通路、间隙连接及跨带突触的结构功能在绵羊卵泡发育中的调控作用，为进一步探明绵羊卵泡发育的调控机制提供参考。     

Effects of phytosterol ester on aortic aging and expression of related genes in rats

AIM: To explore the protective effect of phytosterol ester (PSE) on aortic aging in rats. METHODS: The female SD rats (12 months old, n=42) were randomly divided into control group, model group and PSE group. During the experiment, the rats in control group, model group and PSE group were treated with basic feed, high-fat diet (HFD) and HFD with 2% PSE (W/W) for 6 months, respectively. The morphological changes of the aorta were observed by HE staining and Masson staining, and the absolute area of smooth muscle cells and collagen fiber in the vascular wall were measured by image analysis. The levels of advanced glycosylation end products (AGEs), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in the plasma were detected. The expression of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor γ (PPARγ) at mRNA and protein levels in the vascular tissue was determined by real time PCR and Western blot, respectively. RESULTS: PSE significantly lowered plasma TC and LDL-C, and increased plasma HDL-C level (P<0.05), but had no effect on plasma TG level. PSE significantly attenuated the thickening of intima and media of aging aortic, and decreased the migration of vascular smooth muscle cells (VSMC) and the amount of VSMC and collagen fiber in the aorta (P<0.05). PSE significantly reduced the contents of AGEs and MDA (P<0.05), but had no effect on the activity of SOD and CAT in the plasma. PSE also down-regulated the expression of PPARγ and up-regulated the expression of SIRT1 (P<0.05). CONCLUSION: PSE is able to attenuate the senescence process in the aorta by reducing the production of reactive oxygen species in plasma, and activating SIRT1, or inhibiting the expression of PPARγ in vascular tissues.     

动物营养与饲料学实验教学改革与创新人才培养

根据动物营养与饲料学专业人才培养目标和《甘肃农业大学实践教学指导意见》的要求,通过从动物营养与饲料学实验教学大纲的修订,教学内容的编排,授课形式,授课时间和实验成绩考评等方面进行改革前后的比较与探讨,以期探索提高学生的动手操作能力,培养学生独立思考、发现问题、分析问题和解决问题能力的有效途径.     

涉农专业青年教师以竞赛为平台的专业成长有效途径研究

在信息技术飞速发展的当今社会,对教育工作者提出了越来越高的要求。在专业教师队伍中,青年教师已逐渐成为主体,而想要将这支年轻的队伍打造成一支中坚力量,成为师资队伍建设的重心,也是当今教育资源建设的一项重要任务。本文以涉农专业青年教师为研究的出发点和立足点,通过搭建各种竞赛平台,研究促进青年教师的专业成长的有效途径。     

Whole-plant Corn Silage Quality Treated with Mixed Acids and its Effect on Sheep Growth Performance

In order to explore different concentrations of mixed acids in improving quality of whole-plant corn silage and their effects on growth performance of Dorper×Han F1 sheep.4 levels of 45% formic acid,45% propionic acid and 10% sodium propionate were mixed,and 0(control),0.3%,0.6% and 1% were added to 4 groups of ripening period whole-plant corn.The results showed that compared with control group,the experimental groups could significantly decrease the proportion of AA/PA in silage (P<0.05);0.3% and 0.6% groups had the better effects.Based on the result of CNCPS,0.6% group significantly improved the PB₃ component (P<0.05),reduced the CC component (P>0.05),and improved the quality of silage.Compared with control group,the experimental groups effectively inhibited the mold and yeast reproduction,improved the aerobic stability of silage.The feeding trial results showed that ADG and F/G of sheep in experimental groups were better than control group,and F/G of both 0.6% and 1% groups were significantly better than that in control group (P<0.05).In conclusion,treatment of mixed acids could improve the quality of whole-plant corn silage,and silage treated with 0.6% levels of mixed acids could significantly improve the growth performance of Dorper×Han F1 sheep.     

基于溯源技术的蔬菜基地管理系统开发与应用

蔬菜是我国重要的农产品,其质量安全问题关系着国计民生,一直是社会关注的重点。当前我国的蔬菜产品质量安全问题仍然十分广泛和严重,问题蔬菜的来源去向不明、责任不清,企业对蔬菜种植基地的管理缺乏有效的信息管理手段,消费者对购买蔬菜缺乏有效可靠的信息获取途径。因此,从信息技术的角度构建了一个基于溯源技术的蔬菜基地管理系统,详细介绍了蔬菜溯源码的设计、系统的开发环境、业务流程与主要功能,并重点阐述以规程控制为农业生产指导的蔬菜安全管理模式。系统的开发与应用,实现了"问题蔬菜"的企业内部溯源,以及消费者通过手机查询蔬菜溯源信息,为蔬菜企业提供了对基地种植进行质量管理的信息化工具,为政府提供了一种溯源系统解决方案。     

参与式教学法在《饲料卫生与安全学》课程教学中的应用

为了激发学生对《饲料卫生与安全学》课程的学习兴趣,提高该课程的教学效果,在教学中引入了参与式教学法。通过吸纳学生参与教学目标内容设计、采用体现学生主体的有效教学活动形式、实行促进学生发展的学习成效评价等方面的研究与实践,激发了其学习积极性和兴趣,同时也提高了教学质量。