绿色农药分散剂GCL4-1的结构特征及应用性能研究

采用红外光谱、电位滴定、凝胶渗透色谱对改性木质素磺酸钠农药分散剂GCL4-1的分子结构进行了研究,结果表明,GCL4-1分子中磺酸基含量较改性前的木质素磺酸钠LS有明显提高,磺化度从改性前的1.33 mmol/g增加到1.96 mmol/g;其重均分子量为9700,数均分子量为2300。进一步研究了GCL4-1对农药水分散粒剂(WG)应用性能和药效等的影响,发现以GCL4-1作分散剂制备的WG具有良好的润湿性和崩解性,WG热贮前后的悬浮率都超过90%;WG悬浮液分散稳定性优良,2 h后的悬浮液沉淀层厚度仅为0.386 mm,颗粒粒径为7.22 μm。制得的80%烯酰吗啉WG防治黄瓜霜霉病的EC90值为58.32 mg/L,80%氟虫腈WG样品对小菜蛾的LC50值为0.5196 mg/L,室内毒力达到了国外同类制剂最好产品水平。GCL4-1的综合性能达到甚至超过Borregaard公司的Kinsperse126,是一种具有优良性价比的绿色农药分散剂。     

动物铁过负荷的临床和实验室诊断技术

为动物铁过负荷建立简单、快捷的临床和实验室诊断技术,利用临床自然病例,采用病理解剖学、组织学和组织化学染色技术,对病猪及病犬的淋巴结、脾脏、肺脏、肝脏和肾脏进行了病理解剖学和组织病理学观察和研究.结果表明,随着铁过负荷的程度不同,富含巨噬细胞的淋巴结呈现出由棕黄色至棕褐色的不同色泽;在H.E染色的组织学标本中,可见巨噬细胞内含有数量不等的棕黄色含铁血黄素颗粒;在普鲁氏蓝染色的组织学标本中,含铁血黄素颗粒则呈蓝色.动物发生铁过负荷时,过剩的铁主要沉积在单核巨噬细胞系统的胞浆内,其次是肝细胞的胞浆内.     

正交法优化解淀粉芽孢杆菌B1619水分散粒剂加工工艺

解淀粉芽孢杆菌Bacillus amyloliquefaciens生防菌株B1619对设施蔬菜土传病害有较好的防治效果。为了最大限度降低加工工艺对生物杀菌剂B1619水分散粒剂中含菌量的影响,作者通过单因子水平筛选和正交试验,对B1619水分散粒剂主要助剂配比和颗粒制备条件进行了优化。结果表明:3种主要助剂的最佳配比（质量分数）组合为:硅酸镁铝1.0%、萘磺酸盐甲醛3.0%、硫酸铵4.5%,优化后颗粒含菌量比初始含菌量提高了152.0%;最佳颗粒制备条件为:筛孔粒径1.5 mm、烘干温度65℃、烘干时间20 min,优化后颗粒含菌量比初始含菌量提高了160.0%。验证试验表明,加工工艺优化前B1619水分散粒剂颗粒的含菌量为2.50×108 cfu/g,优化后颗粒的含菌量为1.05×109 cfu/g,优化率为320.0%,优化效果十分显著。     

非洲猪瘟病毒MGF110-5L-6L蛋白诱导宿主细胞翻译阻滞和应激颗粒形成的作用机制

【背景】非洲猪瘟（African swine fever,ASF）是由非洲猪瘟病毒（African swine fever virus,ASFV）感染引发的一种猪烈性传染病,是全球公认的养猪业“头号杀手”,至今尚无安全有效的疫苗和药物。病毒作为专性细胞内寄生物,必须通过“劫持”宿主翻译系统为病毒蛋白合成服务。其中翻译起始因子eIF2α作为翻译调控的核心节点,控制细胞应激反应和翻译重编程走向,对病毒毒力、嗜性、致病性及免疫逃逸等具有重要影响,eIF2α磷酸化调控无疑是病毒与宿主细胞竞争翻译资源的重要阵地之一。然而,关于ASFV编码蛋白与eIF2α磷酸化作用关系的认知极度匮乏。【目的】探究非洲猪瘟病毒MGF110-5L-6L蛋白对宿主细胞翻译阻滞和促进应激颗粒形成的作用机制,为深入揭示非洲猪瘟病毒的致病机制研究提供科学依据。【方法】在前期利用荧光素酶报告基因载体和绿色荧光报告载体,筛选发现外源表达MGF110-5L-6L极显著上调eIF2α磷酸化水平的基础上。选择猪肺泡巨噬细胞3D4/21和猪肾细胞PK-15作为研究用细胞系,利用质粒转染和特异性化学药物处理等方法,结合免疫印迹和激光共聚焦...     

降糖灵颗粒成型工艺的研究

〔摘要〕目的筛选出最佳的降糖灵颗粒剂成型工艺条件〔方法采用单因素考察方法,以其成型性、溶化性、吸 湿性等为评价指标,筛选出其处方中加入辅料的种类、配比及最佳的成型工艺条件、结果确定最佳成型工艺条件 为干浸膏粉与辅料的比例为2:3,用50Ic PVPk 加入90%乙醇溶液制颗粒,其用量为320Ic-360Ic〔结论降糖灵颗粒 的优化成型工艺合理、可行、     

LH Plays a Role to Enhance the Nuclear and Cytoplasm Synchronization During In vitro Maturation of Ovine Oocytes

This study is aimed to investigate the effect of luteinizing hormone (LH) on maturation and fertilization in vitro of ovine oocytes.The ovine oocytes were cultured in vitro in medium with or without LH,and then checked by confocal laser scanning microscope to observe the distribution of cortical granules stained with FITC-LCA during different maturation periods.Similarly,some in vitro matured oocytes were also fertilized in vitro for analysis of their developmental potentiality further.After being cultured in vitro for 4 h,there were significant differences about the rate of germinal vesicle break down (GVBD) between the treatment (with LH) and the control groups (without any hormones) (36.76% vs 18%,P<0.05).Further,there were also significant differences of the cleavage and blastocyst rates between these two groups (67.15% vs 42.37%,21.9% vs 12.71%,P<0.05,respectively).The distribution of cortical granules appeared to spread from the edges to the central site of sheep oocytes following their delaying durations of maturation in vitro.It can be concluded that LH may play a role to delay the occurence of GVBD,prolong the maturation duration of cytoplasm,and enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro and finally improve their in vitro developmental potentiality.     

基于机器视觉技术的苦豆子颗粒外观质量评价研究

利用机器视觉技术建立一种苦豆子颗粒外观质量评价方法,并将该方法用于苦豆子颗粒生产中产品的外观质量评价,以此提高苦豆子颗粒剂生产质量控制水平。该方法首先对苦豆子颗粒进行数字图像采集、特征提取、特征降维,以用于建立苦豆子颗粒外观特征图谱。然后将参比颗粒外观特征图谱与苦豆子颗粒对照指纹图谱进行相似度计算,以相似度值为标准对参比颗粒外观质量进行评价。本研究中依据中国药典中颗粒剂有关要求,对不同主药含量、不同粒度、不同水分的苦豆子颗粒分别进行了外观质量评价。根据相似度计算结果以及数字图像采集误差建议将0.8700作为苦豆子颗粒外观相似度评价的相似度合格值,即苦豆子颗粒生产中外观质量评价的相似度值不得低于0.8700。该方法具有设备简单、操作简便、快速等优点,可以用于苦豆子颗粒生产中产品外观质量评价。     

高效液相色谱法测定加味麻杏石甘颗粒剂中盐酸麻黄碱的含量

建立加味麻杏石甘颗粒剂中盐酸麻黄碱的含量测定方法并测定其含量。采用C18色谱柱,以0．01mol／L磷酸二氢钾（用磷酸调pH至2．5）-甲醇（97：3）为流动相,流速为1．0mL／min,检测波长为210nm。盐酸麻黄碱在0．2～1．2μg范围内线性关系良好,r=0．9986;平均回收率为100．36％,RSD为1．38％（n=5）。     

马铃薯全粉干燥品质的试验研究

采用不同的干燥工艺,以干后马铃薯全粉的感官品质和颗粒的变形为指标,对马铃薯全粉进行了试验研究;得出了风温、干燥方法对马铃薯全粉品质的影响规律。试验结果表明：在本试验范围内,保证马铃薯全粉品质的最佳风温为95 ℃;对撞流干燥所得的产品品质比气流干燥、烘箱干燥、滚筒干燥的效果好。     

Evaluation of in-furrow fungicide treatments to control rhizoctonia root rot of wheat

The efficacy of eight fungicides, placed in-furrow at sowing, to reduce rhizoctonia root rot and increase yields of wheat was examined in glasshouse and field experiments. Under glasshouse conditions three of the fungicides, coated on clay granules at a rate equivalent to 400 g a.i. ha⁻¹, reduced root rot of wheat grown in soil inoculated with Rhizoctonia solani. None of the chemicals stimulated growth of wheat in the absence of disease, but three retarded growth. Experiments at six sites with different soil types and rhizoctonia risk in north-west Victoria, Australia, showed that the fungicide-coated granules were generally ineffective at reducing both incidence and severity of rhizoctonia root rot of wheat. However, tebuconazole was effective at two sites and at one of these flutriafol, RH-7592, cyproconazole and diniconazole also reduced disease severity. Yields were increased by 51% with tebuconazole at one site, by 40% with diniconazole at another and by between 23 and 30% with RH-7592, diniconazole, flutriafol and flutolanil, at a third site.