放养密度和投饲率对南美白对虾单产的影响

为了提高养殖南美白对虾的单产水平,采用梯度设计法和回归分析法研究了放养密度和日投饲率对养殖南美白对虾单产的影响.结果 表明,新疆北疆地区南美白对虾单产(Y)与放养密度(SD)呈极显著二次曲线关系Y=-0.0461SD2+ 11.204SD-78.959(R2=0.9707)(F＞Fn01),当地南美白对虾最佳放养密度为...     

山区河流生态河道治理工程扰动区植被群落恢复的影响研究

工程扰动严重破坏原有地形地貌和生态植被,加剧生态环境的恶化与生态系统的退化,为减少水土流失和浅层滑坡,加速植被恢复和生态修复,对工程扰动区植被群落恢复进行研究是比较有意义的课题.研究选择生态治理后处于自然恢复期的石葵河作为研究对象,对工程扰动区不同水位的植被恢复进行季节性监测和实地调查,统计河道边坡植被恢复种类及数量、...     

"雅士勇"口服液对猪的射精量和精子畸形率的影响

以大白猪和长白猪为研究材料,对照组不饲喂"雅士勇"口服液,两试验组分别饲喂25 mL和30 mL"雅士勇"口服液,研究"雅士勇"口服液对猪的射精量和精子畸形率的影响。结果表明,"雅士勇"口服液对射精量无明显影响(P>0.05),但对种公猪的精子畸形率有显著影响,对照组的精子畸形率均极显著地高于两个试验组(P<0.01),而两试验组之间精子畸形率差异不显著(P>0.05)。     

两个水稻DH群体发芽期和幼苗前期耐碱性状QTL定位比较

利用两个不同的加倍单倍体群体,在0.15%Na2CO3胁迫下,以发芽期和幼苗前期的发芽势（GE）等10个性状碱害相对值作为耐碱指标,进行耐碱性状QTL定位比较。碱害相对值相关分析表明,多个性状的碱害相对值存在显著或极显著相关。采用QTLMapper1.6统计软件对碱害相对值进行QTL分析。DH-1群体定位到10个主效QTL和15个上位性QTL,DH-2群体定位到14个主效QTL和15个上位性QTL。两群体定位列线比对发现：1个控制相对根长的QTL,qRRL3-1,两群体定位在第3染色体对应的区域（CT339-G62和RM7-RM3280）。其他性状两群体没有定位在相同的区域,但存在多个重要的数量基因座位,如CT158-CT550、RM3755-RM418和RM1349-RM1061等区域。在这些相同基因座位上两群体都检测到控制不同性状的主效QTL或上位性QTL,耐碱性QTL可能存在多效性、连锁性,在不同遗传背景下加性效应和上位性效应可以相互转化。多个QTL不仅与水稻耐碱性有关,还与多种抗逆性有关。这些结果将有利于耐碱性分子机制的剖析和强耐碱性水稻品种的选育。     

半定量RT-PCR方法研究microRNA393在旱处理后的孕穗期水稻叶片和穗部的表达情况

microRNAs是新近发现的一类具有重要功能的小分子RNA.研究表明,一些miRNAs与植物抗逆反应相关.本文使用半定量RT-PCR的方法,研究了miR393在旱处理后的孕穗期水稻叶片和穗部的表达情况.结果发现,miR393在胁迫后的叶片和穗部表达上调.同时也发现,miR393在胁迫前后穗部的表达略高于其在叶片中的表达.实验结果也表明,半定量RT-PCR适用于分析miRNAs的表达.     

干旱胁迫下臭椿4种源幼苗光合特性比较

设置4个土壤含水量水平W1、W2、W3、W4,分别为田间持水量的12．5％（严重干旱）、25％（中度干旱）、50％（轻度干旱）、75％（适宜含水量水平,CK）,处理盆栽4个种源臭椿苗未,在苗未速生后期,采用英国CIRAS-1型便携式光合测定系统测定幼苗的光合特性。结果表明：在盆栽土壤干旱胁迫下,山东鲁中、河南宝丰种源的臭椿幼苗的净光合速率、蒸腾速率、瞬间水分利用效率和气孔导度均较高,表现出较强的抗旱特性,而宁夏平罗、北京密云种源幼苗的相应指标较低。     

钾肥对紫花苜蓿根颈丙二醛、可溶性蛋白含量与抗氧化系统的影响

为探究钾肥种类及用量对苜蓿(Medicago sativa)抗寒性的影响,本研究以'北极熊'为供试紫花苜蓿品种,采用二因素随机区组试验设计,研究了秋季施用不同钾肥(硫酸钾和氯化钾)及不同施用量(0,50,100,150,200 kg·hm-2 K2 O)对越冬期苜蓿根颈在不同温度(4℃,—20℃)处理下抗氧化酶[过氧化...     

Ultra‐long‐acting recombinant insulin for the treatment of diabetes mellitus in dogs

BackgroundFor the treatment of diabetes mellitus (DM) in dogs, novel insulins with decreased injection frequency while maintaining safety and efficacy are desirable. Insulin fused with immunoglobulin‐fragment‐crystallizable (Fc) has an ultra‐long plasma half‐life because it recycles through cells, protected from proteolysis.HypothesisGlycemic control can be achieved in diabetic dogs with a recombinant fusion protein of a synthetic insulin and canine Fc (AKS‐218d) administered subcutaneously once‐weekly.AnimalsFive client‐owned dogs with naturally occurring DM.MethodsProspective clinical trial in dogs with DM that were recruited from the UC Davis Veterinary Teaching Hospital and local veterinary clinics. Dogs previously controlled using intermediate‐acting insulin q12h were transitioned to once‐weekly injections of a preliminary construct identified as AKS‐218d. The dose of AKS‐218d was titrated weekly for 8 weeks based on clinical response and continuous interstitial glucose monitoring. Clinical signs, body weight, serum fructosamine concentrations, and mean interstitial glucose concentrations (IG) over the preceding week were compared between baseline (before AKS‐218d) and during the last week of treatment. Data were compared using nonparametric paired tests.ResultsOnce‐weekly AKS‐218d, compared to baseline twice‐daily insulin therapy, resulted in no significant changes in clinical signs, median (range) body weight (+0.4 kg [−0.5‐1.1]; P = .6), fructosamine concentration (−75 mmol/L [−215 to +126]; P = .4), or mean IG (+81 mg/dL [−282 to +144]; P = .8). No adverse reactions were reported.ConclusionControl of clinical signs, body weight, and maintenance of glycemia was achieved with this once‐weekly novel insulin construct in 4 of 5 dogs.     

β-Carotene from the Alga Dunaliella bardawil Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet

Vitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich Dunaliella bardawil alga as the sole source of vitamin A on obesity-associated comorbidities and adipose tissue dysfunction in a diet-induced obesity mouse model. Three-week-old male mice (C57BL/6) were randomly allocated into two groups and fed a high-fat, vitamin A-deficient diet supplemented with either vitamin A (HFD) or β-carotene (BC) (HFD-BC). Vitamin A levels in the liver, WATs, and BAT of the HFD-BC group were 1.5–2.4-fold higher than of the HFD group. BC concentrations were 5–6-fold greater in BAT compared to WAT in the HFD-BC group. The eWAT mRNA levels of the Mcp-1 and Cd68 were 1.6- and 2.1-fold lower, respectively, and the plasma cholesterol and triglyceride concentrations were 30% and 28% lower in the HFD-BC group compared with the HFD group. Dietary BC can be the exclusive vitamin A source in mice fed a high-fat diet, as shown by the vitamin A concentration in the plasma and tissues. Feeding BC rather than vitamin A reduces adipose tissue macrophage recruitment markers and plasma lipid concentrations.     

枯草芽孢杆菌菌剂对盐胁迫下冬小麦生长与土壤水氮分布的影响

为了研究枯草芽孢杆菌菌剂在盐胁迫下对冬小麦生长与土壤水氮分布的影响,以盆栽冬小麦种植为试验手段,在8 g/kg的盐分质量比下,设置枯草芽孢杆菌菌剂质量比为0(CK),1(G1),3(G3),5(G5)和7(G7)g/kg等5种施加梯度处理,分析了冬小麦株高、叶面积、生物量以及土壤水分和硝态氮含量的变化情况.结果表明:①...