大通主要花卉灌木观赏价值评价

大通林区自然分布众多野生灌木花卉植物，通过对其中主要种类的陈述分析，认为这是一些很珍贵的观赏资源，目前应当对这些品种进行引种驯化研究。     

雅安市古树名木现状调查及保护措施探析

通过对雅安市六县两区古树名木资源现状调查,摸清了全市古树名木现有数量、种类、分布等状况,分析了当前古树名木的保护现状和存在的问题,探讨了新形势下古树名木保护管理的对策措施。     

基于对外汉语教学的汉语动补式合成词分析

动补式合成词在汉语中是非常特殊的一类词语,在对外汉语教学中也是难点所在。对外汉语词汇教学需要依照汉语词汇大纲的要求,教师有必要对动补式合成词在汉语词汇大纲中的分布情况和汉语学习者在学习动补式合成词时的难点问题进行分析,并以此为依据制定切实可行的教学策略。研究发现四种动补式合成词在汉语词汇大纲中的分布情况有所差别,各个级别的汉语学习者在不同阶段也存在不同的难点问题,形成了不同教学阶段的重点,教师可以将各种有效的教学对策结合使用,以引导汉语学习者正确习得动补式合成词的意义和用法。     

血尔宝补铁蛋白咀嚼片对大鼠胚胎致畸作用的研究

了解血尔宝补铁蛋白咀嚼片对SD大鼠胚胎的影响,将SD大鼠雌雄1:1交配,发现阴栓作为妊娠“零”天,检出的“孕鼠”随机分到阴性组、阳性组、高剂量组、中剂量组、低剂量组,并称重和编号。于妊娠第7～16d灌胃;受孕的0、7、12、16、20d称体重,并计算给受试物的量。孕鼠于妊娠20d处死,剖腹取子宫称重、取胎记录并检查胚胎、着床数、吸收胎、死胎和活胎数。活胎鼠记录胎仔体重、体长、尾长并检查胎鼠外观有无异常。半数活胎固定于鲍音氏液中固定两周后徒手切片作内脏检查,另半数活胎以茜素红作骨骼染色,并逐步检查骨骼。结果表示,20d孕鼠体重增长阳性组与阴性组、剂量组相比有显著差异(P<0.05);16d、20d孕鼠体重增长高剂量组与低剂量组相比有显著差异(P<0.05);各组相比着床总数无显著差异;活胎的仔数、吸收胎数、死胎数、平均仔重、体重、及尾长,阳性组与阴性组、剂量组相比有显著差异(P<0.05);阴性组、剂量组相比无显著差异(P>0.05);胚胎外形畸形率敌枯双阳性组为58.96%,与其它各组相比有显著差异(P<0.01)。阴性组和剂量组之间无显著差异(P>0.05)。表明血尔宝补铁蛋白咀嚼片对SD大鼠胚胎无致畸、毒性作用。     

高校校办产业模式辨析

本文对新兴的高校校办产业模式进行了较系统的分析，归纳出自我发展、联合开发、集团化、转让开发及建立高科技园区等几种模式。阐明了校办产业在依托本校科技优势，正确处理社会需求与社会限制、主动开拓与被动适应矛盾等方面的共性特点，从中得出推动校办产业发展的有益启迪。     

C1qa deficiency in mice increases susceptibility to mouse hepatitis virus A59 infection

BackgroundMouse hepatitis virus (MHV) A59 is a highly infectious pathogen and starts in the respiratory tract and progresses to systemic infection in laboratory mice. The complement system is an important part of the host immune response to viral infection. It is not clear the role of the classical complement pathway in MHV infection.ObjectivesThe purpose of this study was to determine the importance of the classical pathway in coronavirus pathogenesis by comparing C1qa KO mice and wild-type mice.MethodsWe generated a C1qa KO mouse using CRISPR/Cas9 technology and compared the susceptibility to MHV A59 infection between C1qa KO and wild-type mice. Histopathological and immunohistochemical changes, viral loads, and chemokine expressions in both mice were measured.ResultsMHV A59-infected C1qa KO mice showed severe histopathological changes, such as hepatocellular necrosis and interstitial pneumonia, compared to MHV A59-infected wild-type mice. Virus copy numbers in the olfactory bulb, liver, and lungs of C1qa KO mice were significantly higher than those of wild-type mice. The increase in viral copy numbers in C1qa KO mice was consistent with the histopathologic changes in organs. These results indicate that C1qa deficiency enhances susceptibility to MHV A59 systemic infection in mice. In addition, this enhanced susceptibility effect is associated with dramatic elevations in spleen IFN-γ, MIP-1 α, and MCP-1 in C1qa KO mice.ConclusionsThese data suggest that C1qa deficiency enhances susceptibility to MHV A59 systemic infection, and activation of the classical complement pathway may be important for protecting the host against MHV A59 infection.     

Habitat destruction by collectors associated with decreased abundance of rock-dwelling lizards

Declines in biodiversity caused by habitat loss have been well documented on large spatial scales, however, effects of habitat loss on small scales have received little attention. Some common methods of reptile collection, primarily for commercial harvest, result in destruction of cracks, crevices, and other cool, moist microhabitats in desert rock outcrops. We developed a method for identifying habitat destruction associated with reptile collecting. We surveyed lightly and heavily disturbed areas near Phoenix, Arizona to determine if microhabitat loss caused by collectors was associated with decreased relative abundance of reptiles. Of four diurnal lizard species studied, relative abundance of two rock-dwelling species was negatively correlated with level of microhabitat destruction, whereas relative abundance of one ground-dwelling species and one habitat generalist species was not. Habitat destruction caused by collectors may have negative effects, not only at the individual level, but at the population and community levels as well. We recommend regulation of commercial trade in reptiles; disallowing collecting activities that cause habitat damage; increased law enforcement; and educational programs directed primarily at novice collectors.     

金黄色葡萄球菌SSL7蛋白对补体系统的作用分析

为探究金黄色葡萄球菌超抗原样蛋白(Staphylococcal superantigen-like proteins,SSLs)与补体系统相关的生物学特征,利用原核表达系统表达SSL7重组蛋白,并通过补体杀伤试验、Western Blot以及Pull-down对其有关补体系统的生物学特征进行研究。结果表明:1)重组蛋白SSL7可在大肠杆菌感受态细胞BL21(DE3)系统中大量可溶性表达,大小约25ku;2)SSL7可阻断新鲜兔血清对大肠杆菌DH5α的补体杀伤作用;3)SSL7抑制补体激活后攻膜复合体(Membrane attack complex,MAC)的形成,新鲜兔血清无法在金黄色葡萄球菌NCTC 8325菌体表面形成MAC沉积;4)SSL7能捕获健康人血清(Human normal serum,HNS)中的补体C5,阻断补体系统的激活。综上,金黄色葡萄球菌超抗原样蛋白SSL7可以通过特异性结合补体C5阻断补体级联反应,逃避宿主免疫应答以达到致病的目的,为进一步探究SSL7的感染机制、揭示SSLs蛋白功能奠定基础。     

玉米基因雄性不育双杂合保持系的选育研究

本研究从1982年开始,选用玉米ms1、ms2和ms10三个雄性核不育基因以及易位断点与相应ms基因紧密连锁的T4-6b、T9-10a、T4-10f和T6-10（5519）四个易位系作为基础材料，采用细胞遗传技术育成若干个骨干自交系的基因雄性不育双杂合保持系。至今，已选育出黄早四和Mo17背景的ms2基因杂合保持系。保持系与ms2基因不育系杂交，子代     

Neutralizing and Complement-fixing Monoclonal Antibodies as an Aid to the Diagnosis of Equine Herpesvirus-1 Infection

One complement-fixing (C-MAb) and three complement-dependent neutralizing monoclonal antibodies (N-MAbs) were raised against Hisar-90-7 equine herpesvirus-1 (EHV-1) strain. The target antigen of the C-MAb (2A5) and two of the N-MAbs (1H6, 9C4) was identified as a 140 kDa polypeptide in Western blotting. The target antigen of N-MAb (9C6) could not be identified. Purified polypeptides of five EHV-1 strains isolated from different regions and at different times gave intense bands at 140 kDa when reacted with N-MAb (1H6) in Western blots. In sandwich ELISA, all four MAbs captured the viral antigen from clinical materials, giving a reliable and rapid diagnosis of EHV-1 infection in equines.