Molecular cloning and expression of kidney‐type glutaminase from common carp (Cyprinus carpio) and its up‐regulation by glutamine in primary culture enterocyte

Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln.     

内蒙古典型草原草畜系统适宜放牧率的研究Ⅰ.以绵羊增重及经济效益为管理目标

(1)绵羊个体增重与放牧率呈线性负相关,个体最大增重临界放牧率,因季节不同而变化,随着个体增重与放牧率回归系数b绝对值的增大而降低,以始、终放牧期间所得临界放牧率最小,(2.04羊/hm²)。(2)公顷增重与放牧率的关系符合二次曲线,在达到公顷最大增重以前,随着放牧率的增大而增大,之后则随着放牧率的增大而下降,平均543羊/hm²。(3)公顷最大增重并不一定获取公顷最大利润,主要视买卖价格及其差值而定。(4)以冷蒿小禾草为主的退化草原,宜以个体最大增重的适宜放牧率作为管理标准,以使该类草原在利用中得以恢复。     

德系安哥拉兔的生长特性

德系安哥拉兔不仅以产毛量高、毛质好而著称,而且有生长迅速、体成熟早、体型大的特点。六月龄时已达成年体重的87.37％。母兔的初配可在此时进行,公兔可延迟一个月配种。四月龄与六月龄体重和成年体重的相关系数分别为0.505与0.508,这为德系安哥拉兔体重的早期选择提供了有利时机。     

加强专项管理加快专项预算执行进度

该文阐述了切实加强专项管理工作领导的重要性,强调要落实责任,加快专项预算执行进度,提出从业人员要加强学习与培训,努力提高业务素质,切实做好2007年、2008年修购项目验收工作及2010年修购项目实施的前期准备工作。     

秸秆膨化机度电产量的生产参数优化及影响因素分析

本文对影响秸秆膨化机度电产量的主要生产参数—秸秆含水率(X1)、秸秆粒度(X2)和螺杆转速(X3)进行了研究,揭示了度电产量与这些参数之间的内在规律,创建了秸秆度电产量与三个参数之间的数学模型,进行了生产参数优化,并就生产参数对度电产量的影响进行了分析。     

草坪在城市园林绿化中的作用及其发展研究

草坪在现代城市园林绿化中具有重要作用,如维持城市生态平衡、发展社会效益、美化环境等。本文在阐述草坪综合功能的同时,又从因地制宜地开发草坪资源,健全市场,应用新技术等方面就草坪业的发展进行了研讨。     

Vertical distribution of larval Atlantic menhaden (Brevoortia tyrannus) and Atlantic croaker (Micropogonias undulatus): Implications for vertical migratory behaviour and transport

Understanding the interactions among biological and physical processes is essential to determining how the environment affects transport and survival of fishes. We examined vertical distribution in larval Atlantic menhaden (Brevoortia tyrannus) and Atlantic croaker (Micropogonias undulatus) using 126 depth stratified tows in Delaware Bay, USA, during two cruises, in December 2007 and February 2008. Menhaden larvae were 16.8–24.6 and 20.5–26.2 mm standard length in December and February. Corresponding lengths for croaker were 9.3–17.9 and 8.6–19.6 mm. Using empirical observations, and statistically derived models, we explored larval concentration for both species as a function of location, depth, diel period, tidal period, size, and pairwise interactions. Menhaden concentration was best modeled as a function of station, cruise, and interactions between depth and size as well as between station and cruise. No significant differences in larval menhaden concentration were present among tidal and diel periods. Croaker concentration was best modeled as a function of size and interactions between station and diel period, depth and size, cruise and size. Despite tidal period not emerging as a significant model parameter, we observed larger croaker larvae during nighttime flood tides. Our statistical models are consistent with processes of up‐estuary transport for both species, suggesting larvae are increasingly affected by behavioral responses as larvae grow, exhibiting stronger patterns in vertical distribution. The results refine our understanding of the potential importance of size‐related differences in vertical distribution for larval transport in these species. Future research should examine the interactions among size‐specific vertical migratory capabilities, vertical distribution, transport, and retention.     

香果树种子休眠和萌发的生理特性

为了解决香果树Emmenopterys henryi种子萌发困难的问题,使用不同的光照条件较系统地研究了香果树种子休眠和萌发的生理特性.结果表明:香果树种子属典型的光敏感性种子,在黑暗中无论恒温或变温条件下均完全不能萌发;整个萌发过程需1 000 lx光照强度和每天照光16 h;在光照条件下适宜的萌发温度为20℃、25℃恒温,其次是30℃恒温和30℃(日)、20℃(夜)变温.     

Spatial distribution of Antarctic minke whales (Balaenoptera bonaerensis) in relation to spatial distributions of krill in the Ross Sea,Antarctica

The spatial distribution of Antarctic minke whales in the Ross Sea with relation to spatial distributions of their prey – krill – was investigated in this study using generalized additive models (GAMs). Spatial distributions of two species of krill (ice and Antarctic krill) were estimated by GAMs. Three abiotic factors – distance from the continental shelf break (800 m isobaths), the mean temperature and salinity from the surface to 200 m (MTEM‐200 and MSAL‐200), and latitude and longitude – were used as covariates for models of krill. Estimated spatial distributions of krill were then used with other covariates to model the spatial distribution of Antarctic minke whales. In the selected model of Antarctic minke whales, Antarctic krill were more influential than ice krill. The number of Antarctic minke whales increased as the density of Antarctic krill increased to around 1.5 g m^?2. Beyond that, the number of Antarctic minke whales decreased as the density of Antarctic krill increased. High densities of the Antarctic minke whales were estimated along the sea ice edge in the eastern part of the Ross Sea. Specifically, the densities were high in the north of the continental shelf break where low MTEM‐200 and MSAL‐200 and intermediate densities of Antarctic krill were observed. Further data collection is needed to investigate interannual variations and trends in their relationship. The results show that the spatial distribution of Antarctic minke whales is a function of longitude, distance from the shelf break, oceanographic condition (temperature and salinity), and densities of ice and Antarctic krill.     

The potential of temporary shelters to increase survival of the endangered Mexican axolotl

1. Amphibian populations worldwide are in decline. Proactive conservation techniques such as translocations into created and restored wetlands have gained popularity in recent years but may fail owing to high predation and low environmental adaptability.
2. The Mexican axolotl (Ambystoma mexicanum), affected by rapid urbanization, is now possibly extinct in the wild despite millions living in captivity throughout the world.
3. The aim of this study was to explore whether ponds from an artificial wetland can function as temporary shelters for a viable population of axolotls that could be re-introduced into their native ecosystem in Xochimilco once it is restored.
4. Egg-laying and hatching of 11 unique axolotl pairs placed into reproductive enclosures and larval survival to 7 weeks and up to 12 months were examined. Physicochemical and biological parameters were estimated and compared among ponds.
5. The results from this study are encouraging as they suggest that two ponds have adequate conditions for axolotls to reproduce and for larvae and juveniles to survive.