农杆菌在单子叶植物上的研究进展

在植物基因工程中,农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法,但在单子叶植物上应用较少。在此,对农杆菌介导植物的机理与特点、影响农杆菌转化单子叶植物的因素、农杆菌介导转化单子叶植物的研究进展及其应用前景进行了较全面分析。     

玉米3种非组培转基因方法转化外源bar基因研究

本试验用3种非组培型转基因方法，即花粉介导、子房注射、萌动种胚法在玉米上转化bar基因，经大田筛选及PCR和PCR-Southern检测，证明均可获得转化植株。还分析了3种方法的转化机理，并通过转化率与操作简便程度的比较，认为花粉介导优于萌动种胚法，二者又优于子房注射法。     

Hilbert-Huang transform and denoising for partial discharge ultrsonic singals of large motor

Empirical mode decomposition (EMD) algorithm is introduded as the core of the Hilbert-huang transform (HHT), and implementation process of EMD is analyzed. Then data compression denoising algorithm based on EMD is proposed, simulation and experimental signals are used for verification of the effect of EMD. In the same data sources, the comparison of data compression denoising approaches based on the EMD, db2 wavelet and db8 wavelet are conducted. In addition, physical experiment of the same analysis and comparisons are conducted on a running motor in a Chongqing electrical plant. Simulation and experimental results show that data compression denoising algorithm based on EMD can achieve the same denoising effect, or even better than based on db2 wavelet, db8 wavelet. The former is more perfect than the latter in the real signal processing, and denoising based on EMD is not loss of the original signal energy.     

The Ledge Structural Models of FCC:BCC Partially Coherent Interface

The structure of FCC: BCC partially coherent interface is simulated by computer. The results show that the interface structure is varied with the orientation relationships. The structural models of varied orientations are made, which are defined by the lattice parameter ratio afcc/abcc= 1. 26 and ranged from K-S to N-W orientation relationship. The amount of deviation of the apparent habit plane from atomic habit plane {111}fcc is a function of orientation,and ranges from ca. 12 degrees to ca. 30 degrees. Computer simulations also found that the misfit dislocations could not be certain to exiet on the FCC:CC partially coherent interface.     

利用Bt基因和Xa21基因转化获得抗螟虫、白叶枯病的转基因水稻

利用水稻的愈伤组织作受体, 采用PIG基因枪法, 首先成功地将Bt基因[cryIA(b)]连同抗除草剂bar基因导入栽培水稻品种中国91, 选育出纯合稳定的株系T91系. 然后将T91系与黄淮区主栽品种豫粳6号杂交, 并辅以标记基因选择, 选育出纯合稳定、综合性状优良的转Bt基因株系C48. 利用农杆菌介导的转化系统将Xa21基因转入C48, 根据标记     

根癌农杆菌介导的真菌遗传转化研究进展

 农杆菌介导遗传转化成为真菌实现菌种改良、新基因标记及目的基因的筛选、分离和克隆的重要方法之一。该方法操作简单、受体范围广、转化效率高、单拷贝率高及突变体遗传稳定,解决了传统转化方法的瓶颈。利用该方法成功实现了多种真菌大容量高质量突变体库的构建,且在各类真菌突变体库构建的基础上,相关基因的筛选和功能基因的验证工作成为下一步的研究热点,进而为各类病原菌致病机理的深入研究提供依据,为抗病育种等亟待解决的问题奠定基础。     

一个含LoxP-FRT重组酶位点及易于操作的植物表达双元载体pYBA100

在农杆菌(Agrobacterium tumefaciens)介导的植物遗传转化中,常常需要用到植物表达双元载体。为便于基因工程操作并能在获得转基因植物后方便将标记基因删除,我们构建了植物表达双元载体pYBA100,以满足用于生产安全转基因植物的需要。本研究通过融合PCR方法,尽可能去除所有非必需的序列,将pYBA载体最小化。pYBA载体在大肠杆菌中为多拷贝,骨架为3.69kb。我们构建的含NptⅡ植物筛选标记基因的载体pYBA100仅为5.37kb,多克隆位点为22个,方便基因工程操作。载体pYBA100的T-DNA植物筛选基因表达框两侧预留有LoxP-FRT融合位点,方便在获得转基因植物后通过Cre或FLP重组酶将植物筛选标记基因删除。pYBA100载体能于大肠杆菌和农杆菌中自我复制,并成功转化了拟南芥,符合农杆菌介导的植物转基因要求。离体删除实验结果证明,载体pYBA100能经Cre重组酶删除植物标记基因表达框。     

Genetic transformation of pigeonpea with rice chitinase gene

With a long‐term plan to develop transgenic pigeonpea with resistance to fungal disease, the transfer of a rice chitinase gene to pigeonpea [Cajanus cajan (L.) Millsp.] is reported here. The rice chitinase gene harboured in the plasmid pCAMBIA 1302:RChit was delivered via the Agrobacterium‐mediated method to the cotyledonary node explants followed by subsequent regeneration of complete plants on selection media containing hygromycin. Putative transformed pigeonpea plants were recovered with stringent selection pressure and confirmed using molecular techniques. Stable integration and expression of the chitinase gene has been confirmed in the T0 and T1 transgenics through molecular analysis.     

科研机构科技成果的转化机制研究——基于水产科研院所的案例分析

以水产科研院所为典型案例,对科研机构科技成果的转化机制进行了实证分析。收益型成果转化是水产科研院所渔业科技成果转化的主要模式之一,其中包括内生转化和外生转化。文章分别以武汉市农科院水产研究所、中国水产科学研究院渔业机械仪器研究所为分析案例,介绍内生转化和外生转化两种方式在运行机制、推广成效上的不同,以及各模式存在的问题,从而提出加快农业科技成果转化的对策建议。     

转hrap基因马铃薯获得晚疫病抗性研究

马铃薯晚疫病(Phytopthora infestans)是制约马铃薯生产的主要病害之一.为了扩大马铃薯抗病基因源,提高栽培马铃薯的抗病能力,本试验利用根癌农杆菌介导的方法,将由harpinPss引起过敏反应的甜椒中分离出来的一种过敏反应促进蛋白hrap基因导入马铃薯品种"Burbank"中.结果表明,获得的转化植株经...