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11.
AIM:To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism.METHODS:The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope.RESULTS:Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared.CONCLUSION:The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells. 相似文献
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P. Galeffi G. Giunta S. Guida C. Cantale 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(5):479-483
Citrus tristeza virus (CTV) is one of the most destructive citrus virus diseases in the world. The construction of an engineered antibody, EMBL accession number AJ278109, able to specifically recognize its antigen, i.e. the coat protein of CTV, directly on infected plant material without any purification or manipulation of the entire woody plant. The potential uses of this engineered antibody are discussed. 相似文献
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Effects of aqueous extracts and decomposition of Mikania micrantha H.B.K.
debris on selected agronomic crops 总被引:2,自引:0,他引:2
Laboratory and greenhouse experiments were conducted to determine the effects of aqueous extract and the debris of Mile-a-Minute ( Mikania micrantha H.B.K.) on the germination and growth of four test species, viz. tomato ( Lycopersicon esculentum Mill.), Chinese cabbage ( Brassica chinensis L.), corn ( Zea mays L.) and long bean ( Vigna sesquipedalis L.). Germination percentage, radicle length and fresh weight of two crops, Chinese cabbage and tomato, decreased progressively when plants were exposed to increasing concentrations (12.5, 25, 37.5 and 50 g L−1 ) of aqueous extract of Mikania , but did not affect those of corn and long bean seedlings. The emergence of tomato and Chinese cabbage was greatly reduced when Mikania debris was incorporated into the soil, irrespective of the amount of debris and the decomposition period. In contrast, the emergence of corn and long bean seedlings was not affected. The application of fertilizer (NPK 15 : 15 : 15) enhanced the fresh weight of all seedlings tested as compared with those not supplied with the fertilizer. Four compounds were identified in the leaf extract of Mikania , viz. caffeic acid, p -hydroxybenzaldehyde, resorcinol and vanillic acid. 相似文献
14.
黄花蒿粗提物对几种害虫拒食性的初步研究 总被引:10,自引:0,他引:10
以黄花镐为原料进行浸提,经生物活性测定,结果表明黄花蒿Artenisia annua L.粗提物对供试的6种害虫均具有拒食性。其中对黑翅土白蚁Odontotermes formosanus Shiraki、赤拟谷盗Thibolium castaneum Herbst、谷蠹Rhizopertha dominica Fabricius拒食性极强,对棉蚜Aphis gossypii Glover、棉红蜘蛛Tetranychus urticae Koch及豇豆荚螟Etiella zinckenella Treitschke 也具有较强的巨食性。使用黄花蒿粗提物时,以稀释500倍和800倍效果最好,处理与对照之间有极显著差异。 相似文献
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D.J. Robinson M.F.B. Dale D. Todd 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(9):921-928
Infection of potato plants with Tobacco rattle virus by its nematode vector can have different outcomes, including the development of spraing symptoms in progeny tubers. A novel syndrome described here comprises a generalized mottle in leaves on all stems, together with virtually total failure to produce daughter tubers. The outcome of infection depends partly on the potato genotype, but field trials with 15 varieties showed that the incidence and severity of spraing symptoms at three sites differed. There was no evidence that this was due to differences in virulence among the virus isolates at the sites, but it was probably the result of environmental differences that influenced the numbers and activity of the vector nematodes. At the most severely affected site, spraing symptoms were found in all varieties tested, except Record, including several that were not affected at the other two sites. Taking both severity and incidence of symptoms into account, the ranking of varieties was similar at each site. The incidence of spraing symptoms was greater in larger than in smaller tubers, and increased with later harvest dates, but did not increase when early harvested tubers were stored. Tobacco rattle viruswas detected in the roots of many of the weeds growing at one of the sites and in the roots of a few plants of the subsequent barley crop. 相似文献
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了解土壤肥力对烟田土壤保育具有积极的指导意义。本研究采集了四川攀西山区4个代表性植烟县(区)的19个典型烟田耕层样品,测定了对烤烟产质量影响大且能反映土壤肥力的指标,利用模糊综合评价法计算了土壤肥力综合肥力指数(IFI)。结果表明:19个烟田耕层土壤的pH、有机质、全氮、碱解氮、有效磷、有效钾和水溶性氯含量平均值分别为6.25、21.6 g/kg、1.28 g/kg、120.61 mg/kg、42.84 mg/kg、148.69 mg/kg和7.47 mg/kg,适宜的植烟烟田比例分别为52.67%、26.32%、36.84%、42.11%、36.84%、84.21%和21.05%。烟田土壤肥力综合评价指数介于0.30~0.87,平均为0.55,总体上处于较高水平。施肥上应注意多施钾肥和磷肥并提高其利用率,对氯素低的烟田(<10 mg/kg)可以酌情施用氯肥。 相似文献