Effects of 2 concentrations of sodium citrate on coagulation test results, von Willebrand factor concentration, and platelet function in dogs

BACKGROUND: Blood collection tubes containing 3.2% (0.109 M) sodium citrate, instead of 3.8% (0.129 M) sodium citrate, have recently become available in the United States. These tubes are visually indistinguishable from the traditional 3.8% sodium citrate tubes, except for wording on the label. Consequently, samples for hemostatic evaluation are frequently collected in tubes containing the lower concentration of sodium citrate. HYPOTHESIS: Results of hemostasis assays are different in samples collected in 3.2% versus 3.8% sodium citrate. ANIMALS: Twenty healthy dogs. METHODS: This study aimed at determining whether results of standard coagulation tests, von Willebrand factor concentration (vWF:Ag), and platelet function with the platelet function analyzer PFA-100a were affected by the different concentrations of sodium citrate. Blood samples were collected in tubes containing either 3.2% or 3.8% sodium citrate concentrations and processed routinely for coagulation assays (one-stage prothrombin time [OSPT], activated partial thromboplastin time [aPTT], fibrinogen concentration, and platelet count), vWF:Ag, and platelet function assays with a PFA-100. RESULTS: There was no significant difference between samples collected in 3.2% versus those collected in 3.8% sodium citrate for OSPT, aPTT, fibrinogen concentration, platelet count, or vWF:Ag. The closure times with collagen/adenosine diphosphate were significantly shorter (66 +/- 8.1 versus 74.8 +/- 9.7 seconds; P < .0001) with the 3.2% than with 3.8% sodium citrate concentration, and the hematocrit was significantly higher (47.9 +/- 5.6 versus 46.0 +/- 4.7 seconds; P = .03) in samples collected in 3.2% than in those collected in 3.8% sodium citrate. CONCLUSIONS AND CLINICAL IMPORTANCE: There is no clinically relevant effect of collection of blood into 3.2% or 3.8% sodium citrate.     

基于"3S"技术的甘肃子午岭自然保护区土地利用变化研究

以甘肃省子午岭自然保护区为研究对象,运用"3S"技术,对研究区的地形图、不同时相的TM遥感影像进行数据预处理,并分类提取了研究区2个时段的土地利用信息.对分类信息进行统计分析后并与实地调查数据进行比较,结果表明,近20年来甘肃子午岭自然保护区的森林覆盖面积在稳步提高,乔木林面积明显增加.为甘肃子午岭自然保护区工程的建设提供了基础信息,为林区植物种群生长及分布调查研究提供了宏观数据,并且输出了研究区不同时相土地利用专题图.     

Optimising cutting method and timing for the control of Abutilon theophrasti seed production

Abutilon theophrasti (velvetleaf) is a widespread and problematic annual weed. Greenhouse and field experiments were conducted to investigate the effects of different cutting methods on the viability of A. theophrasti seeds. Three cutting methods were assessed: (1) Entire plant cut and dried (EPD)—plants were cut at soil level and dried with capsules attached on the greenhouse bench or soil surface for 4 weeks; (2) capsules detached and dried (CD)—capsules were removed from plants and dried for 4 weeks; and (3) capsules detached and tested while fresh (CF)—a control treatment. Before drying, the developmental stage (stage one, dark green; stage two, light green; stage three, yellowish-green; or stage four, black with the slightly open capsule) and age (days after flowering, DAF) of each capsule was recorded. Seed viability was measured immediately in the CF treatment and after the 4-week drying period in the EPD and CD treatments. No seeds in the EPD and CD treatments were viable when harvested at the first developmental stage (1–8 DAF) in either experiment, but 100% of seeds in the CF treatment in the field were viable when harvested at 8 DAF. In both greenhouse and field experiments, seeds attained full viability at earlier harvest ages in CF than in EPD or CD treatments, suggesting that seeds might become viable relatively early in development but lose viability if allowed to dry. These findings could be applied to optimise late-season mechanical control of A. theophrasti.     

光源、性别及光处理时间对草地贪夜蛾趋光行为的影响

为研发高效、精准的监测防控设备,利用八臂行为选择装置系统测定不同光源、性别和光处理时间下草地贪夜蛾Spodoptera frugiperda的趋光行为。结果显示,在紫外光（波长365 nm）、紫光（波长420～430 nm）、蓝光（波长465～475 nm）、绿光（波长525～535 nm）、黄光（波长585～595 nm）和红光（波长655～665 nm）6种波长光源下,草地贪夜蛾混合种群（雌雄成虫比为1∶1）对紫外光光源的趋光选择率最高,为25.61%,显著高于其他处理,其次为紫光和蓝光光源,趋光选择率分别为16.30%和8.82%,对红光的趋光选择率最低,为4.76%。在紫外光刺激下,草地贪夜蛾雌成虫的趋光选择率显著高于混合种群（雌雄成虫比为1∶1）,在红光刺激下,雄成虫的趋光选择率显著高于雌成虫。另外,在紫外光刺激下,光处理时间60 min草地贪夜蛾雌成虫的趋光选择率显著高于其他处理。表明光源与性别因素均影响草地贪夜蛾的趋光行为,光处理时间对草地贪夜蛾的趋光行为影响较小,且与光源及性别因素有关。     

基于土地利用/覆盖的甘肃省景观生态风险时空变化特征

甘肃省景观类型丰富多样,在中国西部地区的生态安全中起关键性作用,也为当地发展提供着重要的物质基础和生态服务。由于气候变化和人类不合理活动的影响,甘肃省部分地区生态环境的安全与稳定遭到严峻挑战。本研究基于欧洲航空局全球土地利用/覆盖数据（CCI-LC）,利用动态度、转移矩阵方法分析了2005-2019年甘肃省土地利用/覆盖变化特征;通过构建景观生态风险指数并运用空间自相关分析法分析了景观生态风险的空间分布特征。结果显示：1)2005-2019年间,裸地面积减少最多,变化率1.88%,耕地次之,变化率2.31%;草地面积增加最多,变化率1.83%,建设用地次之,变化率191.08%。2）所有土地利用/覆盖类型中,建设用地的动态度最高且在整个研究时期内变化速率都保持在较高水平,综合动态度最大的时期为2010-2019年。3）土地利用/覆盖转移矩阵主要表现为耕地、林地、草地、裸地之间的转入与转出以及其他土地类型向建设用地的转入。4)2019年整体的生态风险值较2005年明显上升。其中低风险区和较低风险区面积最多,转化最频繁;中等风险区、较高风险区和高风险区面积虽然较少,但都存在不同程度的增长。...     

适期收割对稻谷损失影响的研究

稻米原粮爆腰率是影响加工过程中出米率的重要因素。经调查，农家交售原粮中爆腰率高达25％，其中有干燥、储藏方面的原因，也有收割期不当的原因。本研究从一株稻穗中谷粒含水率与爆腰率的关系分析着手，通过对田间不同品种、不同含水率稻谷爆腰率的测定，并用概率统计方法进行分析的结果证明，完整谷粒的平均含水率在20％以上时收割，可以有效地避免田间爆腰率的发生。     

新城疫病毒La Sota株HN基因的克隆与序列分析

旨在从分子生物学角度进一步研究新城疫病毒La Sota株HN基因,探究NDV La Sota HN基因的遗传变异情况。研究以试验室冻存的p NDV-HN为模版,根据Gen Bank中登录的NDV La Sota株序列设计引物扩增HN基因,将其克隆到pMD18-T载体后进行鉴定,对鉴定正确的重组质粒p MD-NDV-HN进行测序分析。应用生物信息学软件对新城疫病毒Lasota株HN基因进行系统进化树分析、氨基酸序列同源性分析、糖基化位点、蛋白结构跨膜区分析及磷酸化位点预测分析。核苷酸序列测定结果表明:HN基因的序列长度为1743bp,该基因的开放阅读框(Open Reading Frame,ORF)总长为1716 bp,编码571个氨基酸。生物信息学表明:试验获得的HN基因具有6个潜在糖基化位点及12个半胱氨酸残基,第5个潜在糖基化位点(508-510 aa)发生缺失及第123位半胱氨酸残基被色氨酸残基取代。La Sota株HN基因编码的蛋白质中有29个丝氨酸、10个酪氨酸和16个苏氨酸可能成为蛋白激酶磷酸化位点。将试验获得的La Sota株HN基因序列和推导的氨基酸序列与新城疫毒株的HN基因相应序列比较后发现,它们的核苷酸序列同源性和氨基酸序列同源性最高均可达到99%,表明HN基因在种属间具有较高的保守性。研究为新城疫病毒的分子病毒学研究奠定了基础。     

植物开花光周期反应的分子调控机制

植物开花时间受到日照长短季节性变化的调节,拟南芥和水稻中与光周期反应相关基因的分离,使人们得以认识植物开花光周期反应的分子调控机制。植物感知日照长短的变化主要由CONSTANS(CO)基因的表达所控制。CO能够将光信号与生物钟信号整合,调节开花基因FLOWERING LOCUS T(FT)的表达,并最终控制植物的开花时间。本文对这一研究的最新进展进行了综述。     

Two Types Modules for the Boiler Attached to 330 MW Unit

Boiler is an important facility in power plant , the safety and economy of unit is affected by it. Studying the dynamic of boiler can improve the knowledge about boiler , which is important for. Features of MMS are introduced based on MMS simulation setting, and the real time capable module and non real time capable module are compared from principle. Real time capable dynamic model of boiler and non real time capable dynamic model of boiler of 330 MW unit are established . The experimental results show that the dynamic process of two type model accords with the mechanism of the object , at the same time, the validity of real time capable module is discussed .