关于发展渝西地区农机专业合作社的思考——以重庆荣昌县为例

近年来,随着农村改革的深入,荣昌县农机专业合作组织呈现出强劲的发展势头,较好的发挥了农业机械在农业生产中的作用,提高了农机户在农机作业市场中的竞争能力和农业机械利用率。文章以荣昌县为例,对渝西地区农机专业合作社发展特点、问题、方向进行探讨。     

我国海钓基地建设推进路径的探讨

在当前我国海钓产业兴起之时,基地建设已被列入沿海资源型旅游城市开发利用海钓资源的实事项目之一,如何从理论与实践上解决破坏性建设与重复建设的问题成为当务之急。本文试图从基地建设的产业基础条件研究着手,通过对基地生态、环境影响要素条件的评价,结合具体实证规划研究成果,在国内业界首次提出了海钓基地建设推进路径的全新观点。认为,建立岛礁生物资源保护的长效机制是完善和保障产业基础的最主要途径;实施海洋游钓资源生态补偿制度,是实现基地建设可持续发展的基本对策。     

基于温度控制器的金华酥饼烤箱电气化改造

本文测量并分析了金华传统炉烤制酥饼过程中空气温度和炉壁贴饼前后的壁温,根据经验确定电烤炉的传递函数和参数,利用PID控制方法进行控制电烤箱内部空气温度和控制炉壁温度的结构图的仿真曲线分析,对酥饼电烤箱温度控制器的硬件和软件模块作了初步设计,力求将传统土炉温度的经验控移植到电烤炉。     

Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V_H gene segments, V_H families, J_H and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V_H, D, and J_H genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3n-2, 3n-1 and 3n, suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.     

南朝拟古诗的新变

南朝时期,拟古诗创作非常兴盛,并且发生了一些新变。这些新变主要表现为3个方面：江淹的突破、＂永明体＂和宫体诗的倾向、古意诗的发展与军旅边塞诗的出现。这些新变促使诗歌的艺术格局日趋成熟,也扩大了拟古诗的内涵和承载,对诗歌的发展起到重要的作用。     

Construction of SSR Linkage Map and Analysis of QTLs for Rolled Leaf in Japonica Rice

Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were investigated in Xiushui 79 (P1, a japonica rice variety), C Bao (P2, a japonica restorer line) and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs (qRL-1, qRL-7 and qRL-8-1) were detected with two softwares: WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments.     

太谷地区日光温室温湿度年变化规律的研究

研究了在典型晴天条件下,太谷地区全年中温室内温度和湿度的变化情况。结果表明:温室内的温度和相对湿度日变化趋势大体一致,温度最低值和湿度最高值同时出现在7:00~9:00,温度最高值和湿度最低值同时出现在14:00~16:00。全年中温度的季节变化较明显,在不通风的条件下,春季和秋季的温度相对稳定,24 h日均温分别为19.5℃和15.7℃;夏季日均温达到30.5℃,其中观察日内最高温可达到59.2℃,昼夜温差达到42.1℃;冬季温度较低,日均温在12℃左右,观察日内温度低于10℃的时间在10 h以上,出现明显的低温。从空气相对湿度(RH)最高值、最低值的持续时间和日均值来看,以冬季、春季较高,接近100%,85%以上的高湿时间持续15~16 h,RH日均值分别为82%、80%;夏季温室相对湿度日变化幅度最大,最高值与最低值之间相差72%,日均值为64%,但是白天RH低于40%,并且持续时间达10 h左右;秋季RH差值最小,最高值为75%,最高、最低差值为42%,日均值为57%。     

辽宁省果树生产发展的优势条件

辽宁省是我国水果生产大省之一,历史悠久、种类众多,虽然在多年的栽培历史过程中出现过曲折与沉浮,但总的来说还是在不断地发展和进步。现从辽宁省环境条件、区位特点、消费市场、地方经济、技术基础、特色产业等方面概括了辽宁省果树生产的优势条件,以期能够成为辽宁果业发展的原动力。     

InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

High-density markers are necessary for map-based cloning of dca genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a dce rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.