四级分叉银纳米线的制备与表征

氧化铝模板在二次氧化时以1/的倍数逐步降低,形成一级、二级、三级和四级分叉模板孔洞.用电化学沉积法在此孔洞里生长相对应级别分叉的金属银纳米线.银纳米线的直径呈逐级减小趋势,最粗一端直径为50~60 nm,最细一端直径可以达到5 nm 左右.X射线衍射,透射电镜和扫描电镜结果表明银纳米线具有面心立方结构.这些银纳米线可能存在金属-半导体结,为未来的纳电子器件提供广阔的发展前景.     

实时荧光PCR方法检测禽病毒病的研究

通用探针是实时荧光PCR探针类的一种,具有高度的特异性和灵敏度,可对样品进行准确的定量分析。本试验主要采用该方法对通用探针检测禽病毒病进行了研究,试验结果显示本方法的特异性、灵敏度和Taq Man探针相近,且具有操作简便、费用低廉等优点。     

清水混凝土在施工中的质量控制措施

该文章作者较全面的介绍了清水混凝土在施工中应用的全过程及注意事项;并且提出了相应的质量控制措施。     

保证混凝土施工质量的注意事项

本文主要论述了施工中如何保证混凝土的质量及相应的注意事项。     

扣件钢管式模板支撑的应用

本文结合扣件式钢管脚手架在高、重、大跨度梁板模板支撑中的应用,并根据有关规范规定、现场实际情况及现有材料等,对其进行了探讨和总结。     

香蕉RAPD反应体系的建立

利用改良的CTAB方法从香蕉叶片中提取高质量的DNA。在参考一般RAPD分析反应程序的基础上,经过反复试验,确定适合香蕉PCR扩增程序为：94℃预变性 5min;94℃变性 1min;38℃退火 1min;72℃延伸 2min;变性ó延伸,循环45次;最后72℃延伸 2min。PCR扩增的体系（总体积25μL）为：模板DNA 20ng,dNTP 200μmol/L,10×PCR Buffer2.5μL,引物0.20μmol/L,Taq酶0.75U,ddH2O17.85μL。     

转基因杨树外源基因PCR检测模板DNA的快速制备

用带盖离心管定量取转基因毛白杨组培苗及其室外8a生植株叶片,加入DNA提取缓冲液研磨,离心后取上清液稀释5倍。取1uL作为PCR模板DNA检测杨树外源Bt和NptII基因。PCR产物经琼脂糖凝胶电泳检测,目的条带清晰,可重复性强。     

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests

One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.     

C-CRF模型在农作物名识别中的应用

提出了一种基于层叠条件随机场模型(C-CRF)的农业领域命名实体识别方法。该方法在底层以字作为切分粒度,充分利用农作物特征词典,识别简单命名实体。识别结果作为高层条件随机场的输入,在高层以词作为切分粒度,结合复杂特征,识别嵌套命名实体。实证分析表明该方法获得了较好的结果。     

基于无人机遥感的伐区造林坑穴数量与参数提取

为了挖掘无人机遥感在核查造林成果中的应用潜力,采用无人机遥感技术对福建省将乐县一伐区造林坑穴数量和参数进行自动化提取实验。采用大疆精灵4 Pro型无人机,获取空间分辨率0.01m的可见光遥感数据,经处理后得到正射影像（DOM）、数字表面模型（DSM）和无人机影像点云数据;分别采用基于DOM人工目视解译、基于DOM模板匹配法和基于DSM圆形霍夫变换法进行各类信息提取,比较各种方法在造林坑穴数量和宽度、深度参数提取上的精度和实用性。结果表明：基于DOM模板匹配法得到的坑穴数量正确率为92.60%。基于DSM数据的圆形霍夫变换法坑穴数量正确率为95.15%,坑穴宽度和深度提取值与测量值的决定系数（R2）分别为0.93和0.92,均方根误差（RMSE）分别为1.02cm和1.67cm,该方法不仅可以得到优于模板匹配的数量精度,还可有效提取坑穴宽度和深度参数信息,提高了坑穴参数提取的效率。无人机遥感技术在提取坑穴数量、宽度和深度参数方面能够满足造林坑穴指标核查需求。