流式细胞仪检测CD55与CD59诊断阵发性睡眠性血红蛋白尿症

目的 :探讨 CD55、CD59对阵发性睡眠性血红蛋白尿症 (PNH)的临床诊断意义。方法 :应用流式细胞仪 (FCM)检测人体外周血红细胞、粒细胞、淋巴细胞的表面抗原 CD55、CD59的表达水平。结果 :PNH患者 CD55、CD59表面抗原在红细胞、粒细胞、淋巴细胞上均有不同程度的缺乏 ,尤以红细胞、粒细胞较明显。再生障碍性贫血患者的红细胞、粒细胞、淋巴细胞 CD55、CD59表面抗原标记阳性率与正常对照组比较差异无显著性 (P>0 .0 5)。结论 :流式细胞仪检测 PNH患者外周血红细胞、粒细胞、淋巴细胞 CD55、CD59表面抗原具有重要的临床诊断意义。     

抗牛H—Y抗原单克隆抗体的研制试验

Ｈ－Ｙ抗原是位于Ｙ染色体上的基因位点所编码的细胞膜抗原，能直接或间接地诱导原始性腺分化为睾丸，以牛睾丸组织匀浆作为抗原，免疫ＢＡＬＢ／Ｃ小鼠，用杂交瘤细胞技术制备的单克隆缺本６Ｆ，７Ｈ与部分牛精子呈阳性反应。     

Breeding value of base populations derived from 'contiguous' clusters in perennial ryegrass

Five broad-based breeding populations were produced by polycrossing natural populations sampled in five clusters developed by a hierarchical method with a geographic-contiguity constraint. As a result, each of these breeding populations originates from a single region and the populations within a cluster are more likely to share the same alleles with similar frequencies. A single generation of intercrossing would therefore be sufficient to establish a panmixia equilibrium. These populations were tested for dry-matter yield in plots and compared to control cultivars in two locations. There were some trends of specific adaptation related to the geographic origin of populations, which, on average, compared well to controls. Their half-sib families were evaluated as spaced plants in one location. This allowed genetic parameters and expected response to selection to be computed. Three out of five breeding populations appeared to be promising for the development of new, improved synthetic varieties.     

Inheritance of resistance to Tilletia indica (Mitra) in synthetic hexaploid wheat ×Triticum aestivum crosses

Inheritance of resistance to Karnal bunt was investigated in the crosses of four resistant synthetic hexaploid wheats (SH; Triticum turgidum×T. tauschii) and two susceptible T. aestivum cultivars. The resistance was dominant or partly dominant over susceptibility. The SH cultivars Chen/T. tauschii (205) and Chen/T. tauschii (224) have single dominant resistance genes which could be allelic to each other. ‘Altar 84’/T. tauschii (219) appeared to have two dominant genes for resistance. ‘Duergand’T. tauschii (214) possessed two complementary dominant genes for resistance. The work is being extended to involve diverse Karnal bunt-resistant SH and bread wheat cultivars.     

Resistance to Karnal Bunt (Tilletia indica Mitra) in Synthetic Hexaploid Wheats Derived from Triticum turgidum×T. tauschii

Synthetic hexaploids (SH) developed at the International Maize and Wheat Improvement Center (CIMMYT), involving four Triticum turgidum and nine T. tauschii parents, were evaluated for resistance to Karnal bunt (KB) (Tilletia indica Mitra) during three crop seasons over three years at Ciudad Obregon, Sonora, Mexico. Ten tillers of each SH at boot stage, taken at random, were injected with a suspension of sporidia in water (10,000 spores/ml of water). At maturity the inoculated spikes were threshed individually and evaluated for the percentage KB-infected grains. Based on the mean KB score of each entry for three seasons, 49 % of the SH were immune (0 % infection) to KB. Highly resistant expressions characterized the SH which appeared to be influenced by the resistance of their T. turgidum and/or T. tauschii parents. The overall mean infection of the SH wheats was 0.24 % compared to 56.14 % in the susceptible bread wheat check cultivat ‘WL711’. Transfer of KB resistance genes from SH wheats into bread wheat is currently underway at CIMMYT.     

Research on the Law of Highway Debris Flow Moving in Control Structures

Debris flow is a kind of typical water ruins to highways, and it is a dynamic load for highway water ruin. The authors, following the moving law of debris flow and characteristics of highway ruin, put forward reasonable section shape of high - speed drainage trough. Then, according to the energy conservation principle and horizontal throwing theory, the authors established a calculation formula for the throwing distance. The results of the calculation formula given in this paper offer a solution for load calculation and control effect evaluation problems and provide theoretical foundation i to structure design and disease control of debris flow along the highways.     

己烯雌酚人工抗原的合成和酶免疫检测方法的建立

通过化学方法合成己烯雌酚单羧甲基醚,并通过核磁共振和质谱进行鉴定;分别用混合酸酐法和碳二亚胺法将其与牛血清白蛋白(BSA)和鸡卵清蛋白(OVA)偶联,合成己烯雌酚人工抗原,经紫外扫描、聚丙烯酰胺凝胶电泳和动物免疫试验证实人工抗原合成成功,并制备了抗己烯雌酚的特异性多克隆抗体,同时建立了己烯雌酚的酶免疫检测方法,这对动物性食品中己烯雌酚的残留检测有重要意义。     

新疆相对资源承载力与可持续发展问题研究

本文采用相对资源承载力的研究思路和计算方法，分析了新疆1985-2002年相对土地资源承载力、相对经济资源承载力和综合承载力及其演化过程。研究表明：①新疆的土地资源对综合承载力的贡献大于经济资源，是新疆人口的主要承载资源；②1985-2002年，新疆的综合承载力一直超过实际人口数量，处于相对富余状态，但是这种状态星下降趋势；③在西部12个省区市中，新疆的资源承载状况较好，呈现富余状态，仅次于内蒙古，而广西、重庆、四川、贵州4省区呈超载状态；④新疆15个地区(州、市)的相对承载力差异较明显，并且每个地区(州、市)的相对承载力也随着时间的迁移，呈现出一定的规律性变化。针对新疆相对承载力方面存在的问题，提出了新疆实现可持续发展的对策建议。     

黄河水量分配的经济综合模式研究

以水资源分配的伦理经济和效益经济理论为基础,探讨了基于不同目标的水资源分配模式,提出了兼顾伦理与效益的黄河水量分配的经济综合模式,构建了水资源分配的经济综合模型,并以2010水平年为例进行了黄河水量分配的模拟研究和实证分析,提出了黄河流域水资源综合分配方案。该方案与国务院87分水方案相比,提高了流域的总福利水平,同时也维持了流域的和谐发展。     

机群运用效果模糊综合评判方法的研究

本文应用模糊集合理论,建立了机群运用效果综合评判的方法体系。以甘肃省各地市为评判单元,以拖拉机年平均作业量、平均单位油耗、平均标准亩成本及综合机械化程度为评判论域,综合评判结果表明,这一方法是可行的。