Development of a Synthetic Peptide ELISA Assay for the Detection of Foot-and-Mouth Disease Virus Nonstructural Protein Antibodies

In order to develop an ELISA assay with synthetic peptides for the detection of antibody to the nonstructural proteins of foot-and-mouth disease virus, specific peptides were synthesized by a solid-phase method according to FMDV NSPs B-cell epitopes, and were conjugated to carrier protein BSA. An ELISA system was developed to detect FMDV NSPs antibody with the conjugated proteins as the coating antigen. The optimal coating concentration of the antigen was determined as 2.5 μg mL-1. The comparative study of this assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in the detection of 199 serum samples showed that they were very coincident, and the identity rates were 96.48 and 97.48%, respectively. The development of ELISA using the synthetic peptides as coating antigen is specific, reproducible, stable, and easy, and can be used to differentiate FMDV infected pigs from immunized pigs.     

鸡新城疫血凝和血凝抑制试验实训实习效果分析

[目的]对某养鸡场的鸡新城疫抗体进行血清学检测,以有效评估鸡群的免疫状态,制定合理免疫程序。[方法]对永州市某养鸡场病鸡进行鸡新城疫血凝（HA）和血凝抑制（HI）试验,检测鸡新城疫抗体水平。[结果]在检测的60份血样中鸡新城疫抗体效价为1：8—1：4。鸡新城疫抗体效价≥1：16（410g2）的占13．3％,鸡新城疫抗体效价≥1：8（3lo2）的占21．7％,鸡新城疫抗体效价〈1：4（2log2）的占65％。[结论]该鸡场鸡群鸡瘟疫苗免疫失败。     

Evaluation of Aegilops tauschiifor Heading Date and Its Gene Location in a Re-synthesized Hexaploid Wheat

The successful worldwide cultivation of hexaploid wheat in a diverse range of environments is because of, in part, breeding and selection for appropriate heading date. To adjust and fine-tune the heading time of hexaploid wheat to particular geographical regions and specific environment within these, there is an urgent need to evaluate and use alternative alleles for heading time. Aegilops tauschii, the donor species of D-genome of hexaploid wheat, has a wide geographic distribution. The present study revealed a wide variation for heading time among 56 Ae. tauschii accessions. All the accessions with short heading dates belonged to the ssp. tauschii, whereas most of ssp. strangulata accessions showed very long heading date. The heading date was also related to distribution of this species. The monotelosomic and monosomic analysis of a synthetic hexaploid wheat showed that chromosome 2D derived from ssp. tauschii accession AS60 had a major effect on promoting heading time with a reduction of more than 5 days. It is postulated that this Ae. tauschii genotype possess the allele Ppd-D^t1 responsible for the insensitivity to photoperiod. This allele is probably different from Ppd-D1 existing in hexaploid wheat. The new allele Ppd-D^t1 derived from Ae. tauschii might be used as a source for hexaploid wheat breeding on photoperiod response.     

中绒棉96-20高产栽培调控技术探讨

本文通过多年试验、示范、实践，探讨了中绒棉96—20以塑造理想株型并最终获得皮棉160kg／667m^2以上的产量目标所适用的综合调控技术，为大面积种植中绒棉96—20提供指导。     

季冻区旧路改扩建路基加宽技术分析

对季冻区旧路改扩建工程路基拓宽中，容易出现破坏现象的成因进行了分析，重点结合了季冻区的已建或在建的改扩建工程的施工经验，对季冻区的路基拓宽的特殊性进行了技术分析。     

Competition among components of synthetic varieties in alfalfa

Summary Eighteen alfalfa polycross progenies were tested for forage and seed yield in pure stand as well as in competition with the mixture of all progenies included in the test. The type of border affected significantly the forage and seed yield of individual progenies in one third of the cases. In most of these cases competition from the mixture resulted in reduction of the yield of individual progenies. There was no correlation between pure stand forage and seed yield, but there was significant positive correlation between forage and seed yield determined under conditions of competition. It is concluded that polycross progenies should be tested in plots bordered by the mixture of all progenies being tested.     

陆地卫星SPOT、TM数据复合影象在泥石流调查中的应用

将陆地卫星 SPOT数据及 TM数据进行复合 ,制成具有丰富色彩和较高分辨力的复合影象图 ,提取其光谱信息特征和地质体影纹特征。据此 ,对干沟泥石流进行判释和验证 ,查清了干沟泥石流运动特点及发展趋势。