GM1 gangliosidosis in a Japanese domestic cat: a new variant identified in Hokkaido,Japan

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.     

几种药剂对脐橙储藏期病害的防治效果比较研究

用7组药剂分别对纽荷尔脐橙做浸果处理,处理至135天,结果显示：百可得 咪鲜胺处理组烂果率7.78%,病情指数4.81,防效75.5%;咪鲜胺处理组烂果率13.33%,病情指数8.52,防效56.6%;百可得 抑霉唑处理组烂果率14.44%,病情指数8.15,防效58.48%;清水处理组烂果率30%,病情指数19.63。百可得 咪鲜胺处理组储藏效果最好,其次是咪鲜胺处理组和百可得 抑霉唑处理组。在脐橙的储藏保鲜中推荐使用百可得与咪鲜胺或抑霉唑混配或咪鲜胺单剂。     

低剂量60Co-γ射线辐照花粉对贵长猕猴桃果实的影响

为了解低剂量60Co-γ射线辐照花粉对贵长猕猴桃果实的影响,以市售猕猴桃商品花粉为材料,采用不同低剂量60Co-γ射线对花粉进行辐照处理,研究辐照花粉对猕猴桃果实生长发育、果实品质及耐贮性的影响。试验结果表明,与对照组（CK）相比：处理组果实乙烯生成速率、呼吸速率增加（CK＜G6＜G9）,果皮硬度减小（CK＞G6＞G9）;G9辐照处理使果实畸果率升高,座果率、单果重、单果种子数下降,果实干物质、可溶性固形物、可滴定酸及Vc含量显著减少;G6辐照处理后果实生长发育及果实品质均与对照组无显著差异。本研究为低剂量60Co-γ射线辐照去除丁香假单胞菌猕猴桃花粉的应用提供了理论依据。     

基于激光雷达扫描的料仓原料储料量测量方法

为提高仓内原料储料量测量自动化和智能化水平,设计了一种基于二维激光雷达扫描的储料量测量装置与系统。本系统采用RPLIDAR S1型二维激光雷达扫描获取不同储料量物料的原始点云数据,通过坐标变换、重叠点提取、滤波、分割等方法对原始点云进行预处理,采用贪婪投影三角化算法将预处理后的点云进行三维重建,获得仓内原料的三维模型,结合物料三维模型和物料的容重获得仓内原料的储料量,从而实现储料量的自动测量。以玉米为试验对象,测量小型储料塔内玉米储料量并对玉米不同储料量进行扫描测量,验证模型的准确性,结果显示：测量结果的平均绝对误差为8.05 kg,平均相对误差为1.52%。研究结果表明,基于二维激光雷达扫描的储料量测量方法是可行的,具有较好的稳定性和测量精度,能够满足实际生产需求。     

Enhancement of revegetation on construction fill by fertilizer and topsoil application: Effect on mycorrhizal infection

The influence of topsoil and fertilizer application on denuded road construction sites was evaluated to assess its contribution to improvement of vegetation re-establishment. the study sites were within a mixed hardwood and conifer forest on crushed, unweathered subsurface material with low fertility and low biological activity. Topsoils were removed from the site, stockpiled and reapplied to the site after construction. the effect of topsoil amendment on plant growth, soil fertility, mycorrhizal infection and an index of microbial biomass were measured in field and greenhouse experiments. Plant growth on the topsoil amended field plots were greatly increased relative to treatments with fertilizer but no topsoil. Three years after establishment, dry weight production on the plots without topsoil treatment was about 40 per cent of the plots treated with topsoil. Greenhouse experiments were designed to compare fresh, dried and stockpiled topsoil. These experiments indicated that storage of the harvested topsoil for five months in a stockpile had minor effects on plant growth, soil fertility, mycorrhizal infection and microbial biomass. Topsoil volumes had to exceed 20 per cent of the total soil volume to achieve statistically significant benefits and higher ratios showed greater benefit. the percentage of mycorrhizal infection was greatest in topsoil treatments without fertilizer. the addition of fertilizer increased growth but reduced the percentage of roots forming mycorrhizae. When the total weight of infected roots was calculated, however, infection was found to be greatest with a moderate level of fertilizer (equivalent to approximately 27 kg N ha⁻¹ and 39 kg P ha⁻¹), and was less in both higher fertilizer treatments and in unfertilized treatments. Topsoil amendment increased microbial biomass nitrogen but fertilizer treatment did not.     

芒果采后主要真菌性病害及防治方法研究进展

芒果是热带、亚热带重要经济作物之一,因其独特的口感和丰富的营养价值深受消费者喜爱。由于芒果采收期正值高温多雨季节、果实采后生理代谢旺盛、采收难度大,采后运输、贮藏过程中易遭受真菌的侵染,成为限制芒果产业发展的重要因素。为防治芒果采后真菌性病害,国内外学者对芒果采后防治技术进行了研究与开发。对炭疽病、蒂腐病和曲霉病3种芒果采后主要真菌性病害的症状、危害、病原菌和发生条件进行了综述,总结了芒果采后真菌性病害的防治方法,并对芒果采后病害防治方法进行了展望,为芒果采后真菌性病害防治技术的发展提供理论基础。     

关于张家口市发展错季蔬菜产业的几点建议

张家口地区拥有发展无公害错季蔬菜适宜的气候条件和社会经济条件.主要关于该地区发展错季蔬菜在人才的培养、山野菜的开发与利用、市场信息的建立、品种创新、恒温库的建设以及加工能力的提升等方面提出了一些合理化意见和建议.     

抗逆、丰产夏谷新品种沧谷3号的选育

沧谷3号是以鲁谷10号为母本,引F3(日朝谷×80056)为父本杂交选育而成。沧谷3号根系发达,茎秆粗壮,抗倒伏、抗旱、抗病能力强,穗大金黄色,叶片功能期长,绿叶成熟,高产、稳产。2001-2002年参加华北地区夏谷区域试验,平均产量4 972.5 kg/hm2,比对照豫谷5号增产0.6%;2002年参加华北地区夏谷区域试验生产试验,平均产量4 812.0 kg/hm2,比对照增产10.1%。2002—2004年在河北省累计推广种植面积达5.5万hm2。     

菜豆采后贮藏期间的生理变化

菜豆采后在贮藏期间失水严重,硬度迅速下降;呼吸速率缓慢上升,跃变高峰出现在第8d;果肉细胞膜透性在采后初期变化缓慢,后期急剧上升;果实叶绿素、蛋白质、维生素C和有机酸的含量一直呈下降趋势,而果实总糖的含量先升后降,室温贮藏8d后菜豆的商业品质迅速下降.     

山东地区夏玉米的高产种植技术

随着我国市场经济的不断发展和农业供给侧的不断改革,玉米价格呈现出大幅度下降趋势, 玉米种植面积也急剧下降。为了提高玉米的产量和效益,科学推广和应用玉米高产种植技术是当务 之急,同时也是玉米产业快速发展的必然趋势。基于以上情况,本文从精细播种、苗期管理、玉米穗期 管理、花粒期管理五个方面入手,研究了夏玉米高产种植技术。希望通过这次研究,为相关种植人员 提供有效的参考。