山葡萄转录因子CBF1基因的克隆及其遗传转化体系

根据山葡萄的CBF1基因序列设计一对特异引物,克隆出山葡萄(Vitis amurensis)栽培品种‘双优’的抗寒转录因子CBF1基因,并将其构建到pBI121表达载体中,获得山葡萄转录因子CBF1基因的植物表达载体,命名为pBI121-ViCBF1,利用农杆菌介导叶盘转化‘巨峰’葡萄以研究高效的‘巨峰’葡萄转化体系。结果表明,以农杆菌LBA4404为介导菌株,以‘巨峰’葡萄叶片为外植体、3 d为预培养时间和4~5 d共培养时间、400 mg·L-1羧苄青霉素,50 mg·L-1卡那霉素筛选浓度转化效果较好,并已筛选到6株转基因植株,PCR检测均为阳性,为提高‘巨峰’葡萄的抗寒性建立了初步的技术基础。     

Distribution, growth and transformation timing of larval Geotria australis in New Zealand

Lamprey, Geotria australis, are widely distributed in New Zealand and are common in streams and rivers of the west coast of both islands, the southern tip of the North Island, the Banks Peninsula and the southern coast of the South Island. In fresh water, lamprey are found in association with all substrate types but are most commonly (> 50% of the sample sites) found in association with finer gravels. There is insufficient evidence to determine any change in historically abundant adult spawning migrations. Spawning, back calculated from larval growth rates, probably occurs late November-December, and emergence at 9.63 mm occurs in January. We estimate that, on average, ammocoetes grow 0.068 mm/day throughout the year, and metamorphose and emigrate to sea between January-May, 3.5 years after spawning at 101 mm (SD = 6.7).     

养殖水环境中氮的循环与平衡

养殖水环境中，由于人为的介入和影响。使得水体中氮的各种形态转化和循环过程中发生了改变。笔者详细地介绍了养殖水环境中氮的各种存在形态，及其相互之间的转化和循环过程，同时总结了前人研究和提出的各种养殖水环境中氮的物质平衡模型，为优化水产养殖和保护水环境提供科学依据。     

经济转型升级背景下福建省海洋文化产业发展研究

福建省是海洋大省,拥有发展海洋经济得天独厚的坚实基础,发展海洋经济将成为福建省实现跨越式发展的重要转型方向。福建省海洋经济的发展为福建省发展海洋文化产业奠定了良好的基础,福建省海洋文化资源丰富,底蕴深厚,未来海洋文化产业必将占有重要地位,并发挥重要作用。该文从福建经济转型升级与海洋文化产业发展的互动机制入手,分析福建省海洋文化产业发展的制约因素,提出福建省发展海洋文化产业的模式及战略举措。     

Plasma levels of vitamin D3 metabolites during parr-smolt transformation of Atlantic salmon Salmo salar L.

Plasma vitamin D and vitamin D metabolites were measured in Atlantic salmon parr during smoltification and after transfer to seawater. The fish were fed commercial feed for 5 months under natural light, and Na⁺/K⁺ ATPase was measured as an indicator of the smoltification status. No significant differences were recorded in the level of plasma vitamin D metabolites. However, a tendency of increasing plasma concentration of 25OHD₃ and also a temporary increase in plasma 1,25(OH)₂D₃ and 24,25(OH)₂D₃ were recorded prior to seawater transfer. The minor changes in plasma levels of the metabolites indicate a role of the vitamin D metabolites during parr–smolt transformation, although we do not know whether the increased levels are caused by increased synthesis of the metabolites or by decreased binding to receptors or decreased excretion.     

耐盐碱基因eIF1A对玉米遗传转化研究

以来自柽柳的耐盐碱基因eIF1A为研究对象,构建其单子叶植物表达载体,并利用农杆菌介导法转化极早熟玉米自交系加2和M1的胚性愈伤组织,创制转eIF1A基因玉米新材料,为耐盐碱转基因玉米育种提供技术和材料支持。结果表明,成功构建eIF1A基因的单子叶植物表达载体pCAMBIA3300-Ubi-eIF1A,共获得33株除草剂抗性植株,其中,12株目的基因和筛选标记基因PCR呈阳性反应,RT-PCR检测表明,目的基因正常转录表达。     

大麦黑粉菌研究进展

大麦黑粉菌呈世界范围分布,是大麦生产中的主要病害之一,可造成严重经济损失,但在中医上又具有药用和食用价值。本文主要介绍大麦黑粉菌的分类及生理特性,大麦黑粉菌与寄主大麦的互作及其侵染机理,大麦黑粉菌的药用和食用价值,大麦黑粉菌转基因技术体系建立和大麦黑粉菌基因组研究的进展。     

基于竞争优势的中国水产品出口贸易转型研究

中国是水产品生产和贸易大国,但还不是水产品生产和贸易强国。在水产品出口贸易中,由于生产成本不断上升、水产品深加工程度不高以及出口市场过于集中、国际贸易保护主义有所抬头等因素,我国水产品出口面临着愈来愈大的竞争压力。在国际竞争格局发生重大变化的背景下,保持我国水产品出口竞争力,必须通过技术进步、规模经营、质量管理和市场多元化战略,形成新的竞争优势。     

基于大学生读者创新需求的图书馆知识服务

随着物联网和云计算等技术的发展,图书馆的服务正经历从文献服务、信息服务到知识服务的转型发展。通过对大学生读者创新需求与图书馆知识服务转型交互作用机制分析,对影响大学生读者创新的多层面因素分析,深刻阐述知识服务战略转型面临的机遇与挑战,提出图书馆知识服务战略转型的策略。     

不同抗生素对刺槐遗传转化受体系统的影响

本试验研究了卡那霉素(Kana)、头孢霉素(Cef)、氨苄青霉素(Amp)、羧苄青霉素(Carb)、红霉素(E)对刺槐形成层为外植体的遗传转化受体系统的影响。结果表明:刺槐形成层诱导愈伤时Cef对受体材料伤害最小,抑菌效果最好,浓度为200 mg/L时能完全抑制农杆菌生长;用Kana作为刺槐转基因植株的筛选,刺槐形成层愈伤组织分化时选择剂浓度为100~125 mg/L最佳,生根培养时选择剂浓度为75 mg/L最佳。