猪源产毒素多杀性巴氏杆菌HN-13株外膜蛋白H的基因克隆与特征研究(英文)

传染性萎缩性鼻炎是养猪业中的一种重要的呼吸道传染病，产毒素多杀性巴氏杆菌是其重要的病原之一，外膜蛋白H（OmpH）在该菌的致病和免疫中发挥重要作用。本研究克隆了产毒素多杀性巴氏杆菌HN-13株的ompH基因，进行了序列分析，并在大肠杆菌中高效表达，用表达产物建立了间接ELISA方法。同时用生物信息学方法对ompH基因及其编码蛋白的结构特性进行了分析和预测，表明OmpH是一种通道蛋白。     

基于尺度不变特征转换算子的水果表面图像拼接方法

水果全表面图像信息是否完整,直接影响水果表面颜色和缺陷检测的结果。该文提出了一种基于尺度不变特征转换(SIFT,scale invariant feature transform)算子的图像拼接方法,实现多视角水果图像的拼接以获取完整的水果表面信息。首先以15°固定间隔旋转水果以获取各视角下的连续图像,在图像2R-G-B通道下实现图像目标和背景分离,并对目标图像进行灰度直方图均衡化以增强其纹理信息,有利于特征点的提取。运用SIFT算法提取图像特征点,因为特征向量数量多、维数高,采用普通的K-D树算法搜索匹配点将消耗大量时间,因此将图像划分为16个区域,通过多次试验可知中间4个区域为特征点是最容易匹配的区域,这样就缩小匹配点可能存在的区域。采用极线几何约束法和改进型随机抽样一致(random sample consensus,RANSAC)算法以提高图像拼接精度,减少匹配时间。根据平移矩阵,对前后图像进行拼接,从而实现水果表面图像的完整拼接。试验结果表明:该算法平均匹配精度提高35.0%,平均拼接时间为2.5 s,较传统K-D树算法缩短67.8%时间,拼接效果还原率为93.9%。该文算法具有一定的尺度、旋转以及仿射变换不变性,适用于随机呈现的不同姿态球状水果图像拼接。该研究可为基于机器视觉的农产品品质检测和等级划分提供科学参考。     

固沙林庇护区内土壤-植物系统的变化特征

用定位实测法,系统研究了科尔沁沙地24龄人工固沙杨树(Populussimonii)林庇护区内土壤-植物系统的变化特征,同时分析了土壤-植物系统中植物特征与土壤养分之间的相互影响程度。结果表明:(1)与对照点(流动沙丘)相比较,林地迎风区6H(H为平均树高)、3H、林地中央、林地背风区林缘、6H和8H处0～20cm层土壤中的粗沙含量降低26%～69%,粉、粘粒含量增加539%～1731%。土壤中全C、全N、速效P含量分别增加68%～1002%,40%～840%和45%～276%。(3)与对照点相比较,各观测点植物群落的物种优势度有所改变,物种丰富度提高了3～16种,覆盖度增加了43%～54%,地上生物量增加了72%～381g/m2。(3)相关分析结果表明,在林地庇护区内的土壤-植物系统中,植物的物种多样性、枯落物量和根量与土壤C、N含量的相互影响关系更为密切。     

Segmentation of Color Image Based on Feature Divergence and Fuzzy Theory

In order to improve general adaptive capability of algorithm,the new color image segmentation algorithm based on feature divergence and fuzzy theory(FDCIS) is proposed.The algorithm introduces feature divergence and fuzzy dissimilarity function into calculation in order to measure the dissimilarity of feature vector,clusters data by means of feature divergence,and accomplishes the merge of image region.The experimental results demonstrate that the color image segmentation result of the proposed approach reduce calculation on large sample of color image,simply and effectively solve over-segmentation of color image,avoid the dependence of the algorithm on initial condition,and hold favorable consistency in terms of human perception.     

Chromosomal location of genes controlling 6-phosphogluconate dehydrogenase,glucose-6-phosphate dehydrogenase and glutamate dehydrogenase isozymes in cultivated rye

Summary The 6-phosphogluconate dehydrogenase (6-PGD), glucose-6-phosphate dehydrogenase (G-6-PD) and glutamate dehydrogenase (GDH) zymogram phenotypes of wheat, rye and their aneuploid derivates were determined. At least three genes involved in the production of 6-PGD isozymes were located on chromosome arms 4RL, 6RL and 2RL of Imperial rye, King II rye and Dakold rye. Evidence was obtained that at least one gene located on chromosome arm 5RS controls G-6-PD isozyme activities in these varieties of rye and one gene involved in the production of GDH isozymes was located on chromosome arm 2RS of Imperial, King II and Dakold rye. The results indicate that the 6-PGD isozymes exist as dimers and monomers. No possible structure of the G-6-PD and GDH could be determined.     

Efficiency of Embodied Technology Spillovers

Technological progress is the key competitive ability of a society.A society's technological progress depends on it's ability of research and development (R&D) and the absorption of R&D spillovers. Since China is a developing country, which is comparatively short of R&D expenditure and activity, it is absolutely necessarily to utilize R&D spillovers. This paper analyses the relation among destination-specific AC (Absorption Capacity) , source- and destination-specific SS (Structural Similarity) and embodied technology spillovers by one-sector and three-areas GTAP models. The conclusion is:Being 'neutral' in nature, the exogenous Hicks-Neutral Technical Progress shock uniformly reduces the input requirements associated with producing a given level of output of stuff.     

Factors Affecting the Ability of the Enterprise Bargaining in the Skills-based Cooperation

In the skills-based cooperation,the authors carry on the questionnaire survey about the factors affecting the ability of the enterprise bargaining.The formal questionnaire is established by exploratory factor anlysis with SPSS11.0 software.After processing data,theauthors gain the factors that influence entrprise bargaining power:learning ability,promises,craftsmanship investment,the final cost.The structure equation model between the four factors and the bargaining power is tested with Amous 4.0 software.The learning ability is fonnd,which conduces to improve the enterprise bargaining power.However,the promises have the obstructive effect.     

Feature Recognition Based on Vector Picture

The technologyof recognition and understanding architecture engineering drawings by computer is a study hotspot in the field that computer isapplied in engineering research. Based on the characteristicof architectural ichnographyand criterionof architectural design fires prevention, the recognizing identification method and main program based on DXF format is proposed. Experimental results show that it has a preferableautomatic recognition results on the architecture ichnography,and effectivelyimproves the work efficiency.     

基于改进MaxViT的辣椒病害识别分类方法

为实现复杂环境下辣椒病害的精准识别和分类,设计了一种适用于辣椒病害识别分类的方法。以辣椒在生长过程中常见的6种病害为分类研究的对象,使用数据增强的方法扩充数据集,提出一种基于MaxViT改进的MaxViT-DF模型,将MaxViT模型中的普通卷积替换为可变形卷积,使模型在提取特征时能更贴近复杂环境下的识别目标;同时在MaxViT模型施加注意力时引入特征融合模块,提高模型的全局感知能力。结果显示,改进的MaxViT-DF模型识别分类准确率达到98.10%,对6种辣椒病害的分类精度均高于95%。与ResNet-34、EfficientNetv2和VGG-16等模型相比,改进模型在收敛速度和分类精度上具有明显优势。以上结果表明,MaxViT-DF模型能够对不同种类的辣椒常见病害进行有效的分类识别。     

加快农业结构的调整迎接入世的挑战

论述了加入世界贸易组织后,对我国农业的挑战,并提出了相应的对策,以及加快农业结构调整,特别是重庆市农业结构调整的方向和途径。