Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

水质快速检测设备的硬软件设计及实现

[目的]研制本质快速检测设备,实现水质的现场多指标快速检测.[方法]对设备的硬件及软件进行了设计.硬件设计包含了1P2303芯片、锂电,Cortex-M3型ARM微控制器、树莓派显示系统、光频转换器等的设计;软件设计则包含了软件设置、项目选项、项目测量结果以及测量数据表格输出等模块的设计;并对水质快速检测设备的可靠性进行了试验检验.[结果]通过对该设计的设备硬件和软件合理搭配连接,从而研发出了水质快速检测设备.用该设备与国家标准要求的设备同时测定某一河道水样的5项指标结果发现,3个样点水样中铁、氨态氮、氟化物、硝酸盐和6价铬含量的两组数据值接近,无明显差异.虽然国家标准法检测下限比快测法高一个数量级,但快速设备的测定结果不影响操作员对水质的判断,说明该水质快速检测设备精密度高;两种测定方法的稳定性比较,结果快速测定法的5个指标数据的相对偏差略高于国家标准法(3.92％),但其测定结果能够较好地满足现场测定要求,说明该水质快速检测设备稳定性良好.[结论]该水质快速检测设备的检测精度和稳定性较高,可满足水质的现场快速检测要求.     

湛江市园林水景的水生植物配置现状及分析

通过对湛江市几个滨水绿地的实地调查,详细研究了湛江市主要园林水景中水生植物的主要形态特性及其在园林中的应用,并讨论了其应用现状及存在的不足。     

传统图形元素在食用菌包装创意设计中的运用

通过研究食用菌包装中传统图形元素的创意设计,可以使食用菌产品与现代消费者的需求更加贴合,进一步拓展食用菌消费市场。融合地域特色传统文化,满足现代人的审美观念,对传统图形元素进行有效创新,并使用科学的方法进行设计,更好的凸显地域特色文化内涵。     

基于稳健设计思想的家具结构优化设计

总结家具结构设计中存在的问题,探索系统、科学、定量且操作性强的家具结构设计方法以提高低质材家具结构强度和稳定性,降低生产成本。引入结构稳健设计思想和田口实验法,利用有限元软件结构模拟分析优化家具结构的外观和强度设计。有限元软件可以降低家具结构设计的复杂程度,提高设计效率;合理利用稳健设计田口实验法可以提高家具结构的稳定性,降低成本。     

基于鲁棒反馈线性化的联合收获机割台高度控制策略

谷物联合收获机割台高度控制非常重要，有效的割台高度控制有助于提高喂入量的稳定性、降低整机各环节的负荷波动。本文提出一种基于鲁棒反馈线性化的割台高度控制策略，该方法可以使割台跟随地面起伏进行俯仰控制调节。首先，基于割台结构和动力学分析建立系统数学模型，选取正弦角度的近似约简条件，将多变量的复杂非线性系统线性转换为典型的非线性系统；通过分析传统的反馈线性化控制研究可控仿射的模型构建方法，在集成鲁棒优化设计控制器基础上，提出鲁棒反馈线性化（Robust feedback linearization，RFL），通过构建灵敏度方程、选取增益来稳定系统；选取液压控制机构，根据控制液压输出的电流参数设计为基于鲁棒反馈线性化控制系统的控制器。将传统的PID控制和本文提出的鲁棒反馈线性化控制进行对比实验，结果表明，在不同行驶速度、地形正弦振幅和地形周期条件下，鲁棒反馈线性化控制下的高度误差均小于传统的PID控制；以3种不同行驶速度在同一起伏地面上行进，鲁棒反馈线性化控制下的高度误差受行驶速度增加的影响小于传统的PID控制。     

Associations of fish with various types of littoral habitats in reservoirs

Fish associations with different types of littoral habitats were studied in four canyon‐shaped reservoirs in the Czech Republic in years 2010 and 2011 by gillnets. Two to three habitats per reservoir–beaches (former meadows), stump fields (former forest) and rubble slopes–were defined and sampled along the longitudinal axis of reservoirs. Effects of reservoir, habitat and locality (position along longitudinal axis) on fish biomass, abundance and species structure were tested for juvenile and adult fish separately. Hierarchical analysis of variance revealed that habitats differed significantly in fish biomass and abundance. Redundancy analysis showed that analysed environmental variables had significant influence on fish community structure. Most variability in community structure was explained by reservoir and then by combination of habitat and slope steepness. Locality position had the smallest influence on community structure. For both adult and juvenile fish total abundance and biomass, the most inhabited habitat was beaches; rubble slopes were the least inhabited. Habitat associations differed among species. Among adults, bream Abramis brama, white bream Blicca bjoerkna and roach Rutilus rutilus were associated with beaches and stump fields, whereas perch Perca fluviatilis, ruffe Gymnocephalus cernuus, asp Aspius aspius and pike Esox lucius were associated with rubble slopes. Bream, white bream, bleak Alburnus alburnus, roach, ruffe and pikeperch Sander lucioperca were associated with beaches among juveniles, whereas the only juvenile associated with rubble slopes was perch. We showed that most common species are associated with distinct habitats and also that utilisation of various littoral habitats differs in general.     

工业大麻割晒机的三维仿真设计

针对当前我国工业大麻割晒机研究现状与发展需求,运用三维软件UG设计了一款工业大麻割晒机,包括夹持输送机构、切割机构、传动机构、机架等。首先对星轮式拨禾机构、往复式切割器等零部件进行三维建模,然后根据各零部件之间的准确约束进行整机装配,并对样机各运转部件进行了干涉检查,仿真结果表明所设计的工业大麻割晒机符合农艺要求,并为后续的虚拟样机运动仿真、割台的结构优化奠定了良好的基础,提高了样机的研发速度。     

设计阶段平面交叉口安全评价方法研究与应用

基于一种不依赖交通事故和交通冲突数据的事前安全评价方法——安全服务水平评价方法,分析了影响公路平面交叉口安全服务水平的客观因素,建立了设计阶段安全评价模型。结合江苏省临海高等级公路的平交口数据进行实例验证,提出了设计阶段平交口安全评价方案及优化流程,改善效果明显。     

基于三维结构的胡杨茎生长模型的研究与可视化

植物生长模拟研究,是利用计算机仿真技术来模拟现实中植物的生长状态。论文以胡杨生长数据为基础,通过实验分析,推出了胡杨树高与胸径之间的关系,提出一种基于三维结构的胡杨茎生长模型,并运用3DMax技术实现胡杨茎生长模拟的可视化,同时给出了从不同角度模拟胡杨茎生长的状态图,从而全面反映了胡杨茎的生长模拟。