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Jinliang Du Liping Cao Rui Jia Zhengyan Gu Qin He Pao Xu Galina Jeney Yuzhong Ma Guojun Yin 《Aquaculture Research》2020,51(4):1398-1405
Streptococcosis, a bacterial disease for multiple fish species, especially tilapia, caused more disruption to the fish culture industry. However, the underlying mechanisms for the occurrence of streptococcosis remain unclear. The aim of this study was to study the effects of streptococcus on the liver of tilapia. In this study, tilapia were injected streptococcal solution (0.05 ml/10 g body weight) and collected blood and liver at 4, 8, 24, 48, 72, 96, 120 and 144 hr after injection. The results showed that the activities of glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), alkaline phosphatase (AKP) and superoxide dismutase (SOD) in the serum of tilapia exhibited irregular changes: increase at first, then decrease and increase again. The levels of total antioxidant capacity (T‐AOC), glutathione (GSH) and malondialdehyde (MDA) showed a decreasing tendency in serum after injection, except GSH at 4 hr. The activity of catalase (CAT) decreased in serum at 4, 24, 120 and 144 hr after injection of streptococcal solution, while the content TP enhanced in serum at 48, 72 and 120 hr after injection. Observations of pathological sections showed obvious damage to the liver tissue structure in response to streptococcal infection. Western blotting revealed an increase in ikkbeta (Ikkβ) protein with prolonged infection time and a decrease in NF‐kappa‐B inhibitor alpha (IkB‐α) and ikappab kinase alpha (Ikkα) proteins. The gene expression of cytochrome P4501A (CYP1A) was downregulated in liver at 24 and 48 after injection, while the mRNA levels of heat stress protein 70 (HSP70) and tumour necrosis factor alpha (TNF‐α) increased at 48 and/or 72 hr. Taken together, our findings confirmed that streptococcus can cause serious damage to the liver of tilapia. 相似文献
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[目的]探讨三黄连合剂对由海豚链球菌感染罗非鱼发病的预防效果,为罗非鱼链球菌病的免疫防治提供参考依据.[方法]分别以含30、60、120 mL/kg三黄连合剂的饵料投喂吉富罗非鱼,连续投喂7d,给药后第8d进行人工感染海豚链球菌,感染后第7d测定脾脏指数,同时于感染后0、12、24 h和7d采集血清样本,检测超氧化物歧化酶(SOD)、溶菌酶(LSZ)、碱性磷酸酶(AKP)、血清补体C3、IgM抗体水平等免疫学指标.[结果]三黄连合剂连续给药7d对吉富罗非鱼血清免疫指标均有一定影响,可显著提高吉富罗非鱼血清SOD活性(P<0.05,下同).人工感染海豚链球菌后第7d,3个三黄连合剂处理组的罗非鱼脾脏指数较感染不给药组明显升高;在免疫指标方面,高剂量组(120mL/kg)罗非鱼的血清SOD活性、AKP活性、LSZ活性、补体C3活性、IgM抗体水平均显著或极显著(P<0.01)高于感染不给药组.[结论]三黄连合剂可减轻因海豚链球菌感染而造成的免疫抑制,保护机体免疫系统,提升免疫力,对罗非鱼感染海豚链球菌有良好的预防效果. 相似文献
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孙林超 《农产品加工.学刊》2006,(11):36-38
对从酸奶中分离纯化出的保加利亚杆菌(LactobacillusBulgaria,LB)和嗜热链球菌(StreptococinsThermopilos,ST)进行耐硒筛选实验,确定嗜热链球菌为耐硒菌种。用浓度梯度法对其驯化,从而得到富硒菌株,并进行富硒实验,初步确定富硒条件:加硒时间为第5小时,硒浓度为16μg/mL,加硒量为10%,培养时间为10h,培养温度为39℃,pH值为7,采用番茄酵母培养基,富硒率为71.6%。 相似文献
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对吉林地区临床分离的猪源链球菌进行分离鉴定和体外抗菌活性试验,为选择治疗猪链球菌病的最佳药物及临床科学用荮提供参考。试验选用11种临床常用药物,对临床分离的36株链球菌进行了MIC测定。结果表明,链球菌对所测的11种药物中有9种产生了不同程度的耐药性,只有泰妙菌素和氟苯尼考对临床所分离的链球菌比较敏感。说明吉林省猪链球菌耐药率普遍较高,养殖场应予以高度重视。 相似文献
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猪链球菌病对磺胺二甲嘧啶一次和多次静注药代动力学的影响 总被引:5,自引:0,他引:5
健康杂种猪6头,体重(31.3±1.8)kg,用于研究猪链球菌病对磺胺二甲嘧啶(SM2)一次和多次静注药代动力学的影响。用重氮化一偶合比色法测定血药浓度,矩量法分析药一时数据,预测方程法模拟多次给药的浓度曲线。结果显示,猪链球菌病使SM2一次给药的清除率显著下降(P<0.05),多次给药的清除率极显著升高(P<O.01),传统的预测方程对病态下SM2多次给药的动力学不适用。本文对病态下清除率变化的原因进行了讨论。 相似文献
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