瓶式平板培养制备稻曲病菌薄壁分生孢子

利用三角瓶装纳马铃薯琼脂培养基制备稻曲病菌薄壁分生孢子。结果发现,瓶装培养基的体积与产孢量无关。各菌株在瓶式培养平板上均能正常形成后代孢子。瓶式培养比常规培养皿平板培养具有更高的产孢效率。产孢平板上产生的孢子收取后,菌落继续培养,新形成的孢子数量大幅下降。由于三角瓶能高效避免污染,因而认为瓶式培养是制备稻曲病菌薄壁分生孢子的理想技术。     

外源抗虫基因对棉花杂种优势的影响

为研究外源抗虫基因对棉花杂种优势的影响,本研究通过对3对杂交棉组合的产量性状和品质性状的分析得出,外源基因的引入,可以提高杂交棉产量,但对棉花品质性状的改变并没有显著的影响。转基因杂交棉组合的产量性状和品质性状的相关性为负相关,产量和产量构成要素之间呈极显著正相关,品质和品质构成要素之间多呈正相关。     

2株优良天麻共生蜜环菌生长条件筛选

筛选出2株昭通本地天麻共生蜜环菌(SNA03、SNA04)菌索生长的最佳条件。采用单因素试验和正交试验法进行筛选,以生物量为考察指标。2株菌菌索生长的最适温度为25℃,暗培养下菌索生长速度最快;单糖(葡萄糖)为2菌株生长的最佳碳源,有机氮中的酵母膏和蛋白胨为最佳氮源,最适无机元素为K2SO4(SNA03)和KH2PO4(SNA04),最适维生素为VB2;最佳营养条件组合为葡萄糖∶酵母膏∶K2SO4(SNA03)或KH2PO4(SNA04)∶VB2=15g·L-1∶3g·L-1∶2g·L-1∶0.005g·L-)1,氮源(酵母膏)是影响菌索生长的最主要因素。来自昭通的2株天麻共生蜜环菌对营养和环境条件的要求基本一致。最佳生长条件的确定,对昭通蜜环菌快速工业化制种及天麻的大规模生产将起到一定的推动作用。     

Sentinel lymph node mapping in canine mast cell tumours using a preoperative radiographic indirect lymphography: Technique description and results in 138 cases

Several sentinel lymph node (SLN) mapping techniques, to detect nodal metastasis in canine tumours have been investigated in the last 10 years in veterinary oncology. The purpose of this prospective study was to describe a reliable, quick, and inexpensive technique for SLN mapping in canine patients affected by cutaneous and subcutaneous mast cell tumours (MCT). Eighty dogs were enrolled in this study for a total of 138 cytologically diagnosed MCTs. Sentinel lymph node mapping was performed by injecting iomeprole peritumorally followed by serial radiographs at 1, 3, 6 and 9-min post injection. A total of 168 SLNs were detected, 90% at first radiograph, 1 min after the peritumoral iomeprole injection, while in the rest of the cases SLN was identified at 3 min. Sentinel lymph nodes detected by the preoperative radiographic indirect lymphography with iomeprole (PRILI) differed from regional lymph nodes in 57% of cases. The PRILI technique detected simultaneously multiple SLNs in the 26% of cases and multiple lymph centers in the 31% of MCTs. To allow the surgical identification of the SLNs, a peritumoral injection of methylene blue was performed at the time of surgery. This study reports a widely available technique for SLN mapping using digital radiographs in combination with a water-soluble medium, representing a cost-effective alternative to other SLN mapping procedures. Based on our results, this technique can be effective for SLNs mapping in dogs with MCTs but further comparative studies are needed to assess its reliability and efficacy in different tumours.     

The sonographic evaluation of lymph nodes

Ultrasound can be used to detect and evaluate both normal and abnormal lymph nodes, as well as aid in biopsy sampling procedures, an important part of staging procedures in cancer patients. Several parameters can be evaluated using ultrasound; lymph node size, margins, echogenicity, echopattern (echotexture), acoustic transmission, presence and distribution of vascular flow, and vascular flow indices. The most diagnostically helpful include the short/long axis ratio of the lymph node, the pattern of distribution of the blood vessels within the lymph node, and to some extent the resistive and pulsatility indices. This review discusses the use of ultrasound for detecting, evaluating, and sampling peripheral, abdominal and thoracic lymph nodes in small animals.     

家牦牛与不同野血牦牛染色体组型的比较分析

本文用外周血淋巴细胞培养法制备染色体标本,比较分析了家牦牛、１／２野血牦牛及３／４野血牦牛的染色体组型。结果表明：家牦牛、１／２野血牦牛和３／４野血牦牛的二倍体细胞染色体数目均为２ｎ＝６０,常染色体为近端着丝粒,性染色体为亚中着丝粒;家牦牛和野牦牛的染色体组型无明显差异。     

江苏沿岸定置张网主要渔获组成以及对经济鱼类幼体的损害分析

深水张网是吕四渔场传统的作业方式,渔获量占江苏省海洋捕捞总量的比重较大。根据2004年4月~2005年11月吕四渔场深水张网渔业监测资料,结合渔业统计数据,分析了该渔业渔获物种类组成及其季节变化,以及主要经济鱼类渔获物种类组成和幼鱼比例。结果表明,各季节深水张网优势渔获物种类组成具有明显的季节变化特征,春季主要以小黄鱼、黄鲫、焦氏舌鳎和葛氏长臂虾为主,夏季为小黄鱼、黄鲫、焦氏舌鳎和银鲳,秋季为小黄鱼、焦氏舌鳎、棘头梅童鱼、灰鲳和银鲳,冬季为小黄鱼、焦氏舌鳎、棘头梅童鱼和斑鰶;小黄鱼和焦氏舌鳎是深水张网渔业常年优势渔获物。主要经济鱼类小黄鱼以6月份平均网产最高,但主要以幼鱼为主,2004年和2005年的平均体重分别仅0.81 g/ind和1.04 g/ind,幼鱼渔获尾数分别占到99.1%和99.8%,累计损害幼鱼尾数分别达1.84×107ind和2.37×107ind;银鲳平均体重3.22 g/ind和3.8 g/ind,累计损害幼鱼尾数分别为4.84×105ind和4.41×105ind。建议对深水张网渔业加强管理,可对该渔业提前一个月休渔,提高网囊网目尺寸,并强化管理力度。     

THE INDUSTRIAL ORGANIZATIONS AND THE L-S-BUSINESS STRATEGIES

This papar presents an evolution model in business behaviors-the L-S Business Strategies,gives some examples and predictions which support these strategies,and analyzes the relationship between the industrial organizations in China and the L-S Business strategies.     

拟南芥Ran2小GTP结合蛋白抗血清的制备及其FITC荧光标记

将原核表达、纯化的Ran2蛋白对新西兰兔进行免疫制备抗血清,ELISA测定其效价为1∶51 200。免疫印迹分析表明,原核表达、纯化的Ran2和拟南芥可溶性蛋白杂交带均在26 ku处。FITC荧光标记抗体检测Ran2绿色杂交带主要出现在细胞分裂间期的核膜上。     

食用优质稻品种主要品质性状稳定性研究

通过对2002年在湖南长沙作一季晚稻和双季晚稻以及在海南三亚冬繁共3次种植的20个优质稻品种60份样品的碾米品质、外观品质和米饭长检测,同时对其中11个品种共33个样品的蒸煮品质化验,分析不同品种在三种环境下种植的各个品质性状的变异系数, 并对20个品种进行聚类分析;按农业部颁布的食用稻品种品质NY/T-593-2002中的籼稻品质指标等级记分标准将11个品种的9个品质性状计算质量指数. 结果显示：不同品种的品质稳定性不同。品质性状的稳定性与品种的质量指数等级有关,在长沙作双季晚稻种植的质量指数达三等的品种,其品质性状相对稳定;在长沙作双季晚稻种植的质量指数达二等的品种,在不适宜环境下质量指数可变为三等甚至四等。不同品质性状的变异系数差异悬殊。各个品质性状的一等至三等达标百分率因品种和环境而变化。