酵母培养物在动物日粮中的应用进展

酵母培养物(Yeast cultures,简称YC)作为动物的补充饲料已经有60多年的历史。YC是一种经过干燥的产品,既含有酵母菌又含有酵母菌赖以生长的培养基,YC含有丰富的维生素、酶、各种营养成分和某些重要的协同因子,在厌氧环境中能保持代谢活性,并能耐受干燥、加热和酸性等应激环境,在动物饲料中已应用多年,它具有使用方便、安全无毒、无副作用等优点。     

不同物理形态开食料对犊牛生长发育、瘤胃发酵及血液指标的影响

本试验在湿热条件下,探究饲喂不同物理形态的开食料对犊牛生长发育、瘤胃发酵及血液指标的影响。试验采用36头荷斯坦母犊牛,随机分为3组(每组12头),分别自由采食颗粒状开食料(PSA)、粉末状开食料(PSB)和多颗粒状开食料(TS)。试验期63 d,其中1~42日龄为正常饲喂牛奶时期(断奶前期);43~49日龄为断奶期;50~63日龄为断奶后期。开食料从犊牛5日龄开始自由采食。在1、42、49以及63日龄晨饲前进行采血和称重,49和63日龄晨饲前胃管法采集瘤胃液。试验期间每天记录采食量和温湿度指数。结果表明,在湿热环境下:1)断奶期和断奶后2周TS组开食料干物质采食量、平均日增重和重料比高于PSA和PSB组,PSB组最低,但差异不显著(P0.05)。2)TS组犊牛初次反刍时间较PSA组提前3.8日龄,较PSB组提前4.7日龄,差异均显著(P0.05)。63日龄时,TS组瘤胃液氨氮含量显著低于PSA组(P0.05)。3)42日龄时PSA组犊牛血液免疫球蛋白G含量显著高于TS组(P0.05)。4)TS组犊牛初始腹泻时间较PSA和PSB组分别晚0.44和1.75日龄,但无统计学差异(P0.05),TS组腹泻率最低。综合得出,湿热环境下,多颗粒状开食料较颗粒状开食料和粉末状开食料更能促进犊牛的生长发育,使犊牛尽早产生反刍行为,从而加快瘤胃功能的完善     

藤茶植株叶果与组培物中二氢杨梅素含量的比较研究

为比较藤茶经组织培养后不同组织中二氢杨梅素的含量,采用高效液相色谱法(HPLC)测定藤茶叶片、果实、悬浮培养液、嫩叶、愈伤组织、老叶中二氢杨梅素的含量。结果表明:嫩叶、叶片、老叶、果实、愈伤组织、悬浮培养液中二氢杨梅素的含量分别为:25.528%、13.709%、13.337%、6.255%、11.616%、0.829%。愈伤组织中二氢杨梅素含量与叶片接近,悬浮液中含量最低,可以尝试运用愈伤组织培养法生产二氢杨梅素。     

传染性支气管炎病毒M41和Ma5的气管环培养特性研究

本试验以鸡胚气管环纤毛运动作为指示系统，研究传染性支气管炎病毒IBV-M41和IBV Ma5的气管环培养特性。用100 TOC-ID₅₀病毒量的两毒株分别感染鸡胚气管环培养物，观察感染后不同时间气管纤毛的存活率。结果表明，IBV-M41毒株致死气管环上皮细胞的速度要比毒株IBV-Ma5快，说明2个毒株的培养特性与毒力一致。     

Microflora in traditional starter cultures for fermented milk, hurunge, from Inner Mongolia, China

Microflora were investigated in traditional starter cultures for fermented milk, hurunge, which are used for fermented dairy products by nomadic families in the Inner Mongolia Autonomic Region, China. The acid‐forming bacteria and yeast counts ranged from 1.8 × 10⁵ to 5.3 × 10⁸ c.f.u./g and from 6.1 × 10⁵ to 3.2 × 10⁶ c.f.u./g, respectively. Sixty‐six strains of lactic acid bacteria and 30 strains of yeasts were isolated and identified from three hurunge samples collected from the nomadic families. Lactococcus raffinolactis was the most predominant lactococci isolated from these samples. The other lactococci were Lactococcus lactis ssp. lactis, Lactococcus lactis ssp. cremoris, and Leuconostoc mesenteroides ssp. cremoris. Two major lactobacilli strains, Lactobacillus plantarum and Lactobacillus casei, were identified. In addition, Lactobacillus kefiranofaciens, Lactobacillus acetotolerans, which grew in 11% acetic acid culture medium, and Lactobacillus homohiochii, which grew in the culture medium containing 16% ethanol, were also identified. The isolated yeast strains were identified as Candida kefyr, Saccharomyces cerevisiae, Kluyveromyces marxianus var. lactis, Candida krusei and Candida valida.     

英语跨文化交际教学的思考

英语教学的根本目的就是为了实现跨文化交际，教师应意识到跨文化教学是英语教学的一个重要环节，在教授语言的同时同步传授文化知识。本文论述了跨文化教学的内容和必要性，阐述了在英语教学中如何进行跨文化教学。     

不同类型开食料对犊牛生长发育和瘤胃发酵的影响

试验旨在研究开食料类型对荷斯坦哺乳犊牛生长发育和瘤胃发酵参数的影响。选取30头刚出生的中国荷斯坦母犊牛,按照体重进行划分区组,随机分配到3个处理组中,各组分别饲喂颗粒状开食料、粉状开食料和口感化开食料。试验共80 d,其中1～50 d为哺乳期,按照正常程序饲喂常乳+自由采食开食料;51～60 d为过渡期,减少常乳饲喂量+自由采食开食料;61～80 d为断奶后期,自由采食开食料。犊牛从出生第7天开始饲喂开食料。每天记录开食料采食量,分别于试验第1天、第50天、第60天和第80天在早晨饲喂前称重犊牛体重,分别于第50天、第60天和第80天在犊牛采食开食料2 h后采集瘤胃液,测定挥发性脂肪酸浓度和NH3-N浓度。试验结果表明,不同物理形态的开食料对犊牛的体重、平均日增重和开食料采食量具有显著影响。给犊牛饲喂口感化开食料和颗粒状开食料显著增加了干物质采食量和平均日增重,以口感化开食料的饲喂效果最佳。给犊牛饲喂不同物理形态的开食料,对瘤胃pH值、乙酸、丙酸和丁酸浓度无显著影响,但是对瘤胃液氨氮浓度和总挥发性脂肪酸浓度具有显著影响。饲喂口感化开食料和颗粒状开食料显著降低了瘤胃液氨氮浓度,增加了瘤胃液总挥发性脂肪酸浓度。因此,在犊牛开食料的选择上,给犊牛饲喂口感化开食料可以提高犊牛生长性能,改善瘤胃发酵功能。     

Effects of butyrate supplementation in antibiotic‐free milk replacer and starter on growth performance in suckling calves

The aim of this study was to evaluate butyrate supplementation of antibiotic‐free milk replacer and starter on growth performance in male Holstein calves. Twenty‐nine calves were divided into two groups. Group C (n = 13) was fed antibiotic‐free milk replacer without supplementation, and Group B (n = 16) was fed antibiotic‐free milk replacer supplemented with butyrate (1.6 % DM of Gustor BP70^®). Starter in Group B contained 0.3 % DM of Gustor BP70^®. The intake of milk replacer was lower in group B than in C (p = 0.07 for the treatment x week interaction). Body weight (BW) and heart girth (HG) in group B was higher than in C during the experimental period (p = 0.07 and 0.01 for the treatment × week interaction, respectively). The duration of the weaning period in group B was shorter than in group C (p = 0.02). β‐hydroxybutyrate (BHBA) was higher in group B than in C (p = 0.04). Insulin like growth factor‐1 (IGF‐1) concentrations tended to be higher in group B than in C (p = 0.07 for treatment × week interaction). Our results show that butyrate supplementation in antibiotic‐free milk replacer and starter exerted positive effects on growth performance in suckling calves.     

Diagnosis of mixed infections with myxovirus influenzae A equi 2 and herpes virus equi 1 among Danish stud horses

Examination of nasopharyngeal secretion and organ material from clinical cases of respiratory diseases in horses, using inoculation of embryonated hen eggs and rabbit and horse kidney cell cultures, resulted in the isolation of influenza virus and herpes virus. In 2 cases, both viruses were present in the same specimen.On the basis of the physio-chemical, cytological and serological criteria, the viruses were found to be identical with influenza virus type A equi 2 and herpes virus equi type 1.The methods for serological diagnosis and characterization of the influenza and herpes viruses are discussed. Detailed serological examinations of blood specimens from the clinical cases confirmed the aetiological significance of the isolates and the presence of mixed infections.Further characterization of a strain of influenza virus was carried out by inoculation experiments on horses. These included reproduction of clinical influenza, reisolation of the strain in casu and a study of the antibody formation.     

Growth, weaning performance and blood indicators of humoral immunity in Holstein calves fed supplemental flavonoids

The primary objective was to test the hypothesis that flavonoids mediate immune response and affect calf performance. Twenty Holstein calves [7 ± 2 days age; 41.4 ± 0.7 kg body weight (BW)] were randomly assigned to four treatments of (i) no; (ii) low (7.3 × 10⁻⁵ g/kg BW); (iii) medium (7.3 × 10⁻⁴ g/kg BW); and (iv) high (3.6 × 10⁻³ g/kg BW) doses of flavonoids intake in a completely randomized design. Calves received the treatments as a tablet until weaning or a daily intake of 680 g starter. After weaning, calves received no supplemental flavonoids and monitored until 120 days of age. The flavonoids were extracted from propolis. Treatments did not affect body length, wither height and the severity of scours. At week 5 of age, BW was higher when calves fed the high compared to the low dose of flavonoids. At week 6, calves fed the high dose of flavonoids had higher BW than those fed no or low doses of flavonoids. The serum immunoglobulin G (IgG) concentrations remained lower at the first 3 weeks of the experiment when calves received the low but not the high doses of flavonoids. At week 4, both medium and low doses of flavonoids moderated serum IgG. At week 8, the medium and high but not the low doses of flavonoids lowered serum IgG. At week 6, calves fed high and medium flavonoids doses had lower blood immunoglobulin M (IgM) than control calves. Results suggest that flavonoids affect the humoral immune response and can improve growth in young calves. This response depended on calf age. Future studies are needed to further evaluate the premise that dietary forages or the main source of flavonoids are helpful for a less stressful weaning in the modern calf raising.