打叶复烤各工序对卷烟主流烟气氢氰酸与苯并[a]芘、巴豆醛释放量的影响研究

选择红花大金元B3F、C3F、X2F 烟叶,对打叶复烤各工序卷烟主流烟气氢氰酸、苯并[a]芘和巴豆醛释放量进行研究。结果表明：各工序上部烟叶卷烟样品的HCN释放量最高,下部烟叶的最低,一润工序较处理前较大幅度提高了上部烟叶氢氰酸释放量;二润工序与一润工序相比,下部烟叶苯并[a]芘释放量降低9.36%;复烤工序与二润工序相比,下部烟叶巴豆醛释放量降低14.96%,中部烟叶苯并[a]芘释放量降低17.29%。综合结果,上部烟叶各工序间样品的主流烟气3种物质的变化强度都明显高于下部烟叶。     

“双创”背景下饲料企业经营主体多维经营研究

随着时代的变迁和世界局势的发展,创新创业逐渐成为了当前的发展新势态和经济发展新引擎。在此背景下,饲料行业需要与时俱进,深入实施创新,驱动发展,促进企业结构调整,优化产能和经营,以实现可持续发展的目的。本文紧密结合“双创”的深刻涵义,就饲料企业经营主体的经营现状及面临的问题展开分析,对企业的多维经营进行探索与研究,提出了一系列具体的对策与思路。     

甲状腺素对猪小肠上皮细胞miR-let-7a基因表达及细胞增殖的影响

miR-let-7a在动物细胞的分化、增殖与凋亡等方面发挥越来越重要的作用。甲状腺激素(TH)作用非常广泛,机体的每个细胞几乎都是TH作用的靶细胞,其可以促进组织分化、生长和成熟。本实验用甲状腺素(T4)浓度分别为(0、0.02、0.03、0.05、0.075、0.1、0.2μmol/L)在体外培养猪的小肠上皮细胞。结果表明:T4处理组的细胞体积形态相对于空白对照组没有明显变化;当T4添加浓度为0.03μmol/L时,细胞的增殖率显著低于其他组(P0.05);当T4浓度为0~0.03μmol/L时,let-7a的表达随着添加剂量的增加而升高,浓度从0.03~0.2μmol/L变化时,let-7a的表达呈现降低趋势,浓度为0.03μmol/L时表达量极显著高于其他组(P0.01)。let-7a的表达量与细胞增殖呈负相关。     

科学养狐 走出困境

本文详细介绍了新源良种狐繁育场引进国内外科研成果,改良提高国产蓝狐质量,加大科技投入,保持和发挥优良种狐的遗传潜力和抓好基础建设,提高饲养管理的科技含量的成功经验。     

Threadfin shad impacts phytoplankton and zooplankton community structures in channel catfish ponds

Plankton community structure and chlorophyll a concentration were compared in twelve 0.1 ha earthen ponds co‐stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in a multiple‐batch culture (initial biomass=5458 kg ha^?1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867; initial biomass=449 kg ha^?1), during the April–November growing season. We used a completely randomized design in a 2 × 2 factorial arrangement to test the planktivore level (presence or absence of threadfin shad) and channel catfish feeding frequency (daily or every third day). Channel catfish were fed a 32% protein feed to apparent satiation on days fed. The presence of threadfin shad affected phytoplankton and zooplankton community structure more than did feeding frequency, and the impact in ponds was more pronounced after 1 July. The numbers of all major groups of zooplankton were lower in ponds with threadfin shad, but were unaffected by the feeding frequency. Chlorophyll a concentration before 1 July was higher in ponds with threadfin shad and unaffected by the feeding frequency, whereas after 1 July it was higher in ponds without threadfin shad and that were fed daily. Phytoplankton community structure after 1 July was dominated by nuisance algal bloom genera of cyanobacteria in ponds without threadfin shad and by Bacillariophyceae in ponds with threadfin shad.     

毛竹资源现状与经营利用浅析

阐述了毛竹的生长特性,经济、生态效益及毛竹资源利用现状。结合新邵县自然地理、交通状况和毛竹产业发展现状,分析了当前新邵县毛竹开发利用存在的一些问题,并对今后毛竹产业的发展与经营利用模式进行了相应的探讨,以期为林农在毛竹生产经营与管理方面提供参考。     

Effects of RNA interference targeting CDC25agene on proliferation of human liver cancer HepG2 cells

AIM: To investigate the effect of silencing cell division cycle 25a (CDC25a) gene on the proliferation of human hepatoma HepG2 cells. METHODS: CDC25agene in human hepatoma HepG2 cells was silenced by RNA interference. Real-time PCR was applied to detect the expression of CDC25a, cyclin E and CDK2 at mRNA levels in the HepG2 cells. Western blotting was applied to detect the expression of CDC25a at protein level. In addition, MTT assay, Giemsa staining and flow cytometry were used to measure the proliferation of human hepatoma HepG2 cells. RESULTS: The expression of CDC25a at mRNA and protein levels in RNA silence group was lower than those in negative control group and normal control group (P＜0.05). The mRNA expression of cyclin E and CDK2 in silence group was lower than that in negative control group and normal control group (P＜0.05). The cell proliferation in silence group was lower than that in negative control group and normal control group (P＜0.05). The results of flow cytometry revealed that the cells in silence group were blocked in G1 phase. CONCLUSION: Infection of LV-CDC25a-RNAi recombinant to the HepG2 cells effectively inhibits the CDC25agene expression and the proliferation of human hepatoma cells, and arrests the cells in G1 phase, suggesting that CDC25agene may be a key target for the treatment of liver cancer.     

Development of a linear mixed-effects individual-tree basal area increment model for masson pine in Hunan Province,South-central China

ABSTRACT

An individual-tree basal area increment model was developed for masson pine based on 26276 observations of 13,138 trees in 987 sample plots from the 7th (2004), 8th (2009), and 9th (2014) Chinese National Forest Inventory in Hunan Province, South-central China. The model was built using a linear mixed-effects approach with sample plots included as random effects since the data have a hierarchical stochastic structure and biased estimates of the standard error of parameter estimates could be a consequence of applying ordinary least square (OLS) for regression. In addition, within-plot heteroscedasticity and autocorrelation were also considered. The final mixed-effects model was determined according to the Akaike information criterion (AIC), Bayesian information criterion (BIC), log-likelihood (Loglik), and the likelihoodratio test (LRT). The results revealed that initial diameter (DBH), the sum of the basal area (m²/ha) in trees with DBHs larger than the DBH of the subject tree (BAL), number of trees per hectare (NT), and elevation (EL) had a significant impact on individual-tree basal area increment. The mixed-effects model performed much better than the basic model produced using OLS. Additionally, the variance structure of the model errors was successfully modeled using the power function. However, the autocorrelation structures were not defined because there was no autocorrelation amongst the data. It is believed that the final model will contribute to the scientific management of the masson pine.     

信息技术环境下的森工企业SCM模式浅析

本文运用SCM的基础理论，阐述了在当前信息技术环境下SCM模式的运用，探讨了目前我国森工企业在运用SCM模式中所存在的一些问题和误区。     

中外人工用材林经营方式比较分析

首先对国内外人工用材林经营方式进行了分析, 进而提出通过林工贸一体化发挥产业间的协同效应, 实现人工用材林由分户经营向规模化经营的转变, 将成为我国人工用材林经营方式的发展趋势; 其次, 对我国人工用材林规模化经营的现实性进行了分析; 最后, 在探讨林工贸一体化实现规模化经营内在机理的基础上, 提出林工贸一体化经营是我国人工用材林规模化经营的实现途径。