EVALUATION OF MAGNETIC RESONANCE SAFETY OF VETERINARY RADIOFREQUENCY IDENTIFICATION DEVICES AT 1 T

Implants containing metallic components have the potential to become heated or move within the patient while in the magnetic resonance (MR) environment. Despite containing a ferromagnetic core and having been in use for over 20 years, no information is available on the safety of veterinary radiofrequency identification devices during MR examinations. These devices are the most commonly encountered metallic implants in dogs and cats undergoing MR imaging. Three commercial veterinary microchips were evaluated for safety in the MR environment at 1 T. Parameters tested were translational force, torque, heating, artifact production, and function. Translation and torque were larger than that expected from normal activity under normal gravity. No significant heating was observed. Signal void artifacts may affect diagnosis if they are too close to the area of clinical importance. Microchip function was unaffected by routine clinical MR imaging. Capsule formation around devices is a major factor in counteracting translation and torque. Our findings support that is acceptable for patients to undergo MR imaging with this 1 T system following an interval of 3 months postimplantation to allow capsule growth. Because of the complex interactions involved, these observations may not be translatable to MR scanners of different field strength and/or manufacturer. Further safety testing of these and other radiofrequency identification devices is therefore recommended at different field strengths and equipment specifications.     

凉山州腹泻仔猪猪圆环病毒2型感染情况调查

为了解凉山州腹泻仔猪猪圆环病毒2型(PCV2)的感染情况,采用PCR方法对采自四川省凉山州西昌市和喜德县7个猪场85份腹泻仔猪粪便、病变组织等样品进行PCV2检测,并将扩增到的PCV2 ORF2基因片段进行测序和序列分析。结果显示,PCV2阳性样品有22份,阳性率为25.9%,阳性猪场占71.4%(5/7)。22个ORF2基因片段长度均为459 bp,核苷酸同源性为92.8%～100%,氨基酸同源性为93.5%～100%。构建的系统进化树显示22个测序序列分别处于2个分支:PCV2b和PCV2d,但未形成明显的地理分支。结果表明凉山州猪群中PCV2感染较为普遍,且以PCV2d亚型为主,并存在一定程度的遗传变异。     

运用贝叶斯方法估计中国美利奴羊(新疆型)毛用性状及繁殖性状的遗传参数

为了解中国美利奴羊（新疆型）近年来遗传结构的变化趋势以及探讨毛用性状与繁殖性状的遗传关系,需要进一步研究这些性状的遗传力以及它们之间的关系。本研究收集额敏县聚鑫细毛羊养殖专业合作社1985-2018年中国美利奴羊（新疆型）共计9 428只羊毛生产记录和1987-2018共计5 887只年繁殖记录,运用BLUPF90软件结合Gibbs抽样方法,利用单性状模型对中国美利奴（新疆型）毛用性状（细度支数、等级、总评分、毛长、污毛重和鉴定时体重）和繁殖性状（配种次数、妊娠天数、胎产羔数和总产羔数）进行方差组分和遗传力估计,利用双性状模型分析毛用性状与繁殖性状之间的遗传相关与表型相关。结果显示,中国美利奴羊（新疆型）毛用性状细度支数、等级、总评分、毛长、污毛重、鉴定时体重的遗传力估计值分别为0.471±0.020、0.088±0.030、0.114±0.018、0.426±0.025、0.328±0.041、0.317±0.046;繁殖性状配种次数、妊娠天数、胎产羔数及总产羔数的遗传力估计值分别为0.056±0.009、0.022±0.010、0.120±0.018、0.163±0.016;毛用性状与胎产羔数、总产羔数之间的遗传相关范围为-0.031~0.286,鉴定时体重与胎产羔数（0.286）、总产羔数（0.204）遗传相关最高,细度支数与胎产羔数（-0.143）、总产羔数（-0.048）呈负的遗传相关;毛用性状与胎产羔数、总产羔数之间的表型相关范围为-0.210~0.216,毛长与总产羔数（0.216）表型相关最高,细度支数与胎产羔数（-0.137）、总产羔数（-0.210）呈显著负表型相关。本研究结果发现,毛用性状与繁殖性状之间存在一定的关系,这一结果可为今后制定中国美利奴羊育种规划提供数据基础,为选育优质高产、繁殖性能好的细毛羊提供理论依据,从而进一步提高细毛羊产业经济效益。     

不同日粮类型和粗蛋白质水平对四川山地乌骨鸡生产性能和屠宰性能的影响

试验旨在研究不同日粮类型和粗蛋白质水平对四川山地乌骨鸡生产性能和屠宰性能的影响。采用2×2因子设计,设2种日粮类型(玉米—豆粕型和玉米—豆粕—酵母蛋白型)和2个粗蛋白质水平(16.5%和14.5%)。选用23周龄的四川山地乌骨鸡480只,设4个处理,每个处理4个重复,每个重复30只鸡。预试期14 d,正试期120 d。结果表明,日粮类型对四川山地乌骨鸡的生产性能无显著影响(P>0.05),日粮16.5%粗蛋白质水平组平均蛋重显著高于14.5%粗蛋白质水平组(P<0.05)。日粮类型与粗蛋白质水平互作可显著影响平均蛋重(P<0.05),但对产蛋率、日产蛋量、日采食量和料蛋比均无显著影响(P>0.05)。日粮类型、粗蛋白质水平及日粮类型与粗蛋白质水平互作对四川山地乌骨鸡的屠宰性能均无显著影响(P>0.05),其中玉米—豆粕—酵母蛋白型(粗蛋白质16.5%)日粮组屠宰率、全净膛率、半净膛率、胸肌率和腿肌率最佳。结果提示,四川山地乌骨鸡的平均蛋重受日粮粗蛋白质水平影响较大;饲喂粗蛋白质水平为16.5%的玉米—豆粕—酵母蛋白型日粮可获得较好的生产性能和屠宰性能。     

南方型紫花苜蓿耐盐细胞系的筛选及生理特性分析

以NaCl为选择因子,对长期继代培养的紫花苜蓿愈伤组织进行选择,筛选出耐1.2%NaCl的耐盐细胞系并对其进行生理分析,结果表明:耐盐细胞系对逆境的忍受能力高于对照,积累的脯氨酸和可溶性蛋白含量也高于对照,维持着较高的K+/Na+比,在盐分胁迫下能维持较高的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。     

PCV2衣壳蛋白羧基端基因在T4噬菌体表面的展示及ELISA方法的建立

用PCR扩增猪圆环病毒Ⅱ型广东分离株的衣壳蛋白羧基端基因,将PCR产物连接到pR质粒,转化DH5α细胞,筛选阳性克隆进行PCR鉴定并测序后,转化E2菌,将重组E2菌与缺陷型噬菌体T4-Z1同源重组后,得到重组噬菌体,经SDS-PAGE和Western blotting分析,表明衣壳蛋白片段在噬菌体表面正确展示,表达的融...     

Ⅰ型鸭肝炎病毒的分离与鉴定

鸭病毒性肝炎(duck virus hepatitis,DVH)是造成养鸭业严重经济损失的传染病之一。作者对松原市某鸭场送检的病死鸭进行病毒分离试验、雏鸭回归试验、DHV分离株ELD₅₀测定、雏鸭保护试验、鸡胚中和试验、鸭胚接种试验和电镜观察等,确诊该病为Ⅰ型鸭病毒性肝炎。试验所分离得到的Ⅰ型鸭肝炎病毒与标准毒株的回归试验病理特征存在一定差异,其基因序列的测定及分析还有待于进一步研究。     

家鸭流感病毒的流行病学研究 Ⅱ.鸭源A型流感病毒对鹌鹑与鸡的致病性

利用６株鸭源Ａ型流感病毒（ＡＩＶ）的鸡胚传１代、２代或３代尿囊液,采用滴鼻－点眼、腹腔注射、静脉注射或肌注－腹注等途径,分别对鹌鹑、商品来航鸡、ＳＰＦ来航鸡进行人工感染。结果,鹌鹑５０％发病,无死亡;商品鸡１００％发病,死亡６２．５％;ＳＰＦ鸡１００％发病,死亡９２．４％。结果显示,腹腔注射和静脉注射最为有效,滴鼻、点眼的致病效果同样确实。本试验发现雄禽比雌禽易感,发病率高。试验表明,鸭源弱致病性流感病毒对其他禽类存在致病潜力,因而具有一定的流行病学意义及生态学意义。     

Morbidity and mortality associated with a Standardbred yearling sale

In the 2‐week period following a Standardbred yearling sale, 5 horses from the sale died or were subjected to euthanasia. Three were confirmed as equine herpesvirus type 1 myeloencephalopathy (EHM), while EHM was suspected in 2. Clinical abnormalities were reported in 77 of 177 (43.5%) other yearlings during the 14‐day period after the sale; however, no diagnostic testing was performed. Eleven secondary cases of suspected EHM in horses in contact with yearlings purchased at the sale were identified. No cases of EHM were identified associated with the same sale in 2003; however, there was no difference in morbidity with clinical abnormalities reported in 39/96 (41%) horses sold that year (P = 0.70). Yearling sales have an inherent risk of pathogen exposure and illness is common in purchased horses. Disease in these horses could have been associated with a wide range of respiratory pathogens; however, the incidence of confirmed or suspected primary and secondary EHM cases associated with the 2002 yearling sale highlight the potential for significant outbreaks of disease.     

我国兔毛品质研究状况的探讨

通过对兔毛纤维类型、物理特性和结构特征的分析,并与相关动物纤维作比较,探讨了兔毛在纺织上的应用价值。