Cytotoxic Effects of Loperamide Hydrochloride on Canine Cancer Cells

Loperamide is a peripheral opiate agonist that can cause apoptosis and G2/M arrest in human cancer cell lines and may sensitize cells to chemotherapy. The objectives of this study were to investigate the effects of loperamide on viability, apoptosis and cell cycle kinetics in canine cancer cells and to establish whether the drug sensitizes cells to doxorubicin. Cell viability was assessed using Alamar Blue. Cell death and cell cycle were studied using flow cytometry with 7-Aminoactinomycin-D (7-AAD) and propidium iodide (PI), respectively. Loperamide decreased cell viability in a dose-dependent fashion and was most effective against canine osteosarcoma cells. In all cell lines, it induced a dose and time dependent apoptosis and resulted in accumulation in G0/G1. When co-incubated with doxorubicin, loperamide induced a synergistic cell kill in canine carcinoma cells. Investigation is warranted into the role of loperamide in the treatment of canine cancer.     

国外X80管道钢管的研究与应用现状

综述了国外在高钢级X80管道钢管方面的开发和应用状况，管材的研究目前正向更高屈服强度、更高韧性和易加工的方向发展，使用高钢级管材是提高管道设计压力的主要途径，同时，在同样的设计压力下使用高强度管材可养活钢材的消耗量。以德国Ruhrags管道为例，介绍了国外高钢级管道钢管X80的实际质量水平，包括管道钢的合金化、控制轧制和控制冷却工艺，并就高钢级管道钢管的部分热点问题如富气输送、高钢级管道钢管的焊接和裂纹扩展止裂等进行了讨论和分析。     

最大熵技术分析湖南汽车工业发展优序

运用最大熵技术对湖南汽车工业部分重点企业进行了优序分析，并以此为基础，探讨了湖南汽车工业应优先发展的产品，针对性地提出了加快发展湖南汽车工业的若干建议。     

高速公路的建设对沿线经济发展影响的研究——以赣粤高速公路江西段为例

以赣粤高速公路建设对江西省经济发展影响为例,阐明了高速公路对沿线经济发展的深刻影响.分析了高速公路建设对江西省区位优势改变、产业结构和产业布局的影响,从而提出了构建沿线经济带的设想.     

强恢复系盐恢559的选育与应用

盐恢５５９是用常规杂交经低世代测恢鉴定筛选育成的籼型三系强恢复系。表现杂种优势强，恢复度高，抗逆性好，制种产量高，与三系不育系已配制出系列杂交稻新组合。讨论了强恢复系的选育方法。     

S_m∨P_n和S_m∨C_n的边共色数

利用Lowell Beineke和Richard Ringeisen给出的边共色数的界,研究得到了Sm∨Pn和Sm∨Cn的边共色数.     

优质晚粳糯稻不育系绍糯9714A的特性与应用探讨

绍糯9714A系绍兴市农科院以滇Ⅰ型不育系7627A作母本,以绍糯9714为轮回父本,经多代回交转育而成,2005年9月通过浙江省科技厅组织的技术鉴定。绍糯9714A属半矮生类型品种,群体不育株率100%,败育花粉率99.95%,以染败为主,米质指标达部颁优质食用稻米二级标准以上。以绍糯9714A所配的粳糯杂交组合米质优、抗病性好,试种示范表现优良,增产显著。     

TRANSTHORACIC LUNG ULTRASOUND IN NORMAL DOGS AND DOGS WITH CARDIOGENIC PULMONARY EDEMA: A PILOT STUDY

Pulmonary edema is the most common complication of left‐sided heart failure in dogs and early detection is important for effective clinical management. In people, pulmonary edema is commonly diagnosed based on transthoracic ultrasonography and detection of B line artifacts (vertical, narrow‐based, well‐defined hyperechoic rays arising from the pleural surface). The purpose of this study was to determine whether B line artifacts could also be useful diagnostic predictors for cardiogenic pulmonary edema in dogs. Thirty‐one normal dogs and nine dogs with cardiogenic pulmonary edema were prospectively recruited. For each dog, presence or absence of cardiogenic pulmonary edema was based on physical examination, heartworm testing, thoracic radiographs, and echocardiography. A single observer performed transthoracic ultrasonography in all dogs and recorded video clips and still images for each of four quadrants in each hemithorax. Distribution, sonographic characteristics, and number of B lines per thoracic quadrant were determined and compared between groups. B lines were detected in 31% of normal dogs (mean 0.9 ± 0.3 SD per dog) and 100% of dogs with cardiogenic pulmonary edema (mean 6.2 ± 3.8 SD per dog). Artifacts were more numerous and widely distributed in dogs with congestive heart failure (P < 0.0001). In severe cases, B lines increased in number and became confluent. The locations of B line artifacts appeared consistent with locations of edema on radiographs. Findings from the current study supported the use of thoracic ultrasonography and detection of B lines as techniques for diagnosing cardiogenic pulmonary edema in dogs.     

城市园林景观设计需把握的几个问题

简要介绍了当前城市园林景观设计在建设实践中的发展现状以及制约其发展的因素,如盲目追求档次、使用功能弱化,地域特色缺失、后期建设难于落实设计等。同时,针对这些因素,提出在今后的设计中应把握的几个问题,如结合地域经济、社会发展等条件,注重实用功能、地域特色、生态功能以及建设可行性等,综合各种因素使园林设计在实践中不断完善。     

Development and Identification of Introgression Lines from Cross of Oryza sativa and Oryza minuta

Introgression line population is effectively used in mapping quantitative trait loci(QTLs),identifying favorable genes,discovering hidden genetic variation,evaluating the action or interaction of QTLs in multiple conditions and providing the favorable experimental materials for plant breeding and genetic research.In this study,an advanced backcross and consecutive selfing strategy was used to develop introgression lines(ILs),which derived from an accession of Oryza minuta(accession No.101133) with BBCC genome,as the donor,and an elite indica cultivar IR24(O.sativa),as the recipient.Introgression segments from O.minuta were screened using 164 polymorphic simple sequence repeat(SSR) markers in the genome of each IL.Introgressed segments carried by 131 ILs covered the whole O.sativa genome.The average number of homozygous O.minuta segments per introgression line was about 9.99.The average length of introgressed segments was approximate 14.78 cM,and about 79.64% of these segments had sizes less than 20 cM.In the genome of each introgression line,the O.minuta chromosomal segments harbored chromosomal fragments of O.sativa ranging from 1.15% to 27.6%,with an overall average of 8.57%.At each locus,the ratio of substitution of O.minuta alleles had a range of 1.5% 25.2%,with an average of 8.3%.Based on the evaluation of the phenotype of these ILs,a wide range of alterations in morphological and yield-related traits were found.After inoculation,ILs 41,11 and 7 showed high resistance to bacterial blight,brown planthopper and whitebacked planthopper,respectively.These O.minuta-O.sativa ILs will serve as genetic materials for identifying and using favorable genes from O.minuta.