一串红新品种‘红色恋人’

‘红色恋人’一串红是从河北省三河市农家一串红种子经4 代连续选择优良单株混合收种后得到的新品种。在早春保护地播种育苗、露地栽培条件下株高53 ~ 70 cm,冠幅72 ~ 90 cm,花序长15 ~32 cm,整株花序数79 ~ 119 枝,轮间距离0.6 ~ 2.7 cm,花萼和花冠红色。花期6-10 月,盛花期8-9 月。较耐热,不易发生疫病和花叶病。     

一串红新品种‘紫冠精灵’

一串红‘紫冠精灵’是从淡紫色花萼深紫色花冠一串红实生群体中选育出的新品种。在露地栽培条件下经4次摘心,株高可达54 ~ 61 cm,冠幅57 ~ 69 cm。花萼淡紫色,花冠深紫色。花序长27 ~ 33 cm;花轮数18 ~ 24轮;整株花序数105 ~ 135枝。北京地区早春保护地播种,露地种植,花期可达150 d;初夏露地播种种植花期可达75 d,夏季高温时生长良好。     

海南腐霉新记录种Pythium splendens的鉴定及其对油棕苗的致病性测定

 华丽腐霉Pythium splendens是我国对外植物检疫对象油棕苗疫病的病原菌,从海南坝王岭自然保护区长臂猿原始林区采集的土样中分离出1株腐霉菌HB0401,经形态特征和核糖体rDNA ITS1序列分析鉴定为华丽腐霉（Pythium splendens Braun）。将该菌株接到健康的油棕苗植株上,能引起典型的油棕苗疫病症状。这是第1次在海南分离到华丽腐霉（Py. splendens）并证实其为油棕苗的致病菌。     

一串红生长和开花特性对高温反应的品种间差异研究

一串红是我国夏秋季节常用的红色草花,温度超过30℃会严重影响其观赏价值。本试验研究了9个一串红品种在田间自然高温下的生育期及田间生长指标的变化情况。结果表明一串红不同品种间在生育期上存在显著差异,火娃和辉虹开花天数最长。在自然高温胁迫条件下,一串红不同品种间在株高、冠幅、花序长度和花序数上存在显著差异,所有品种生长放缓,并且均出现了少量叶片萎蔫的现象,但高温并未对一串红的正常开花起到显著影响。在整体表现上,辉虹、皇后、火娃和红孩表现出较高的观赏价值,有较强的耐热性,适于在高温下生长。     

北京地区常见鼠尾草属植物AFLP亲缘关系分析(英文)

[目的]采用AFLP分子标记技术对北京地区鼠尾草属(Salviaspp.)植物的7个种23份样本进行亲缘关系分析。[方法]利用21对引物组合中筛选出的稳定性好、多态性较高的6对引物(E+ATT/M+AC;E+ATT/M+GA;E+AAG/M+CTG;E+AAG/M+CCT;E+AAG/M+ACT;E+AAC/M+ACT)用于扩增北京地区鼠尾草属植物基因组DNA,反应体系分为两步:①梯度降温扩增。第1个循环为94℃,30s;65℃,30s;72℃,60s。以后每个循环的退火温度依次降低0.7℃,共13个循环;②普通扩增。94℃,30s;56℃,30s;72℃,60s;23个循环。扩增后采用6%聚丙烯酰胺凝胶和BG-Power3500型电源和DYC-20A型电泳槽,在恒功率60W的条件下电泳至溴酚蓝指示线刚刚跑出玻璃板。银染后在灯光下统计扩增条带数,构成二进制矩阵表。用NTSYSpc2.10e软件进行分析,采用UPGMA方法进行聚类分析,通过Treeplot模块生成聚类图。[结果]对23份鼠尾草属植物进行AFLP分析,得到367个扩增位点,其中多态性位点359个,占总扩增位点的97.82%。在遗传距离为0.32的水平下,23份鼠尾草属植物样本归为7个组:一串红(S.splendens)组、蓝花鼠尾草(S.farinacea)组、朱唇(S.coccinea)组、雪见草(S.plebe-ia)组、丹参(S.miltiorrhiza)组、荫生鼠尾草(S.umbratica)组和罗马尼亚鼠尾草(S.officinalis)组。蓝花鼠尾草、朱唇、雪见草、丹参、荫生鼠尾草、罗马尼亚鼠尾草与一串红的亲缘关系依次增远;一串红种内,自选品种(系)与商品种亲缘关系较远。[结论]该研究结果可为北京地区开展鼠尾草属植物的种间和种内远缘杂交提供理论参考。     

芨芨草遗传多样性研究

研究芨芨草的遗传多样性,探讨不同地理种群的遗传分化与地理位置及生境的关系,以期为芨芨草种质资源的开发、利用与保存提供参考。     

芨芨草遗传多样性研究（英文）

[Objective] Study on the genetic diversities of A.splendens from different areas of xinjiang.[Method] The genetic diversities among seven populations and within two populations were analyzed by RAPD.[Result] Genetic clustering results presented that the relationships among populations of A.splendens are directly related with geographic positions,and within population are related with habitats.[Conclusion] RAPD technique can be used to study genetic diversity of A.splendens.     

Replacement of live feed by formulated feed: effect on the growth and spawning performance of Siamese fighting fish (Betta splendens,Regan, 1910)

Partial replacement of live feed (LF) with formulated feed (FF) was performed in Betta splendens to evaluate their growth, survival and reproductive performance. Three hundred B. splendens fry of uniform size (mean weight 0.19 g±0.01) were equally distributed in five treatment groups with each of three replicates in glass aquaria of 35 L capacity. Fish were offered diets of different ratio of LF and FF like T1 (control) – 100% LF; T2 – 75% LF, 25% FF; T3 – 50% LF, 50% FF; T4 – 25% LF, 75% FF and T5 – 100% FF. Highest (P<0.05) body weight gain (% BWG) (67.1±1.03) and specific growth rate (2.34±0.02) were observed in the T2 group. Best feed conversion ratio was also found in T2 (2.40±0.11), which was similar to T1 and T3. The highest survival rate (%) was recorded in T1, T2 and T3 groups (97±1.7), which was similar to T4 and the lowest in T5 (49±2.0). The highest gonadal weight (0.12 g±0.01) and gonadosomatic index (15.17±0.50) was observed in the T3 group during the first sampling after 63 days. No significant changes in spawning performance were observed in the second sampling after 21 days after first sampling. However, T4 group registered the highest fecundity and fertilized eggs at the end of 105 days of experiment. From the present study, it concludes that LF can be successfully replaced to the extent of 25% by FF without any detrimental effect on the growth, survival and spawning performance of B. splendens.     

一串红花粉发育过程中外壁糖蛋白的透射电镜观察

研究采用细胞化学技术,用钉红对一串红5个不同发育阶段的花粉进行染色、经透射电镜对其外壁糖蛋白进行定位观察,探讨花粉发育过程中外壁糖蛋白的发生时期及输送到花粉壁的过程.结果表明:糖蛋白在单核时期由绒毡层细胞产生,并被分泌到药室内,输送到花粉外壁及萌发孔周围积累和贮存,整个过程延续到花粉成熟.     

多效唑对一串红穴盘苗育苗质量的影响

以一串红穴盘苗为研究材料,研究了多效唑在一串红穴盘苗上施用的最佳时期及浓度。结果表明,三叶一心期50 mg.L-1处理为最佳施用时期及浓度,可以比对照降低株高40%,增加茎粗23%,增加叶片厚度154%,增加全株干重35%,根系活力提高77%。最终,壮苗指数比对照增加了177%,主要是由于株高的降低,茎粗和叶片厚度的增加促进了同化物质的积累,使幼苗强壮。