云南白雪茶研究现状及开发利用

云南白雪茶是滇西北高寒地区民间特有的一种长期使用的药物和保健饮品,具有茶文化特征.从云南民间的食用经验来看,白雪茶具有显著的清热、止咳、安神等保健作用,现代药理学研究又证明,白雪茶还具有抗炎、解热、抗疲劳、抗辐射、促进免疫等药理学活性.根据雪茶的研究现状对其植物学特性、化学成分、保健作用作了简要的阐述,为以后的开发利用提供了理论依据.     

观赏植物菌根真菌多样性研究进展

菌根真菌具有丰富遗传多样性、形态多样性、物种多样性、生态系统多样性和功能多样性。而观赏植物是重要的依赖菌根真菌的植物类群,对于美化环境、改善生态条件、促进经济发展等方面均具有重要价值,人们给予了众多研究,其中,观赏植物菌根真菌多样性研究表明观赏植物菌根真菌具有丰富的形态、物种和功能多样性。本文系统总结了国内外观赏植物菌根真菌多样性研究所取得的成果、存在的问题,并探讨了今后研究方向,旨在为进一步开展观赏植物菌根研究与应用提供依据。     

不同光质LED光源对鱼腥草幼苗膜脂过氧化及抗氧化酶的影响

以鱼腥草为材料,以日光灯处理为对照,分析了不同光质LED光源(白、红、黄、绿、蓝LED灯)对鱼腥草叶片活性氧、类黄酮含量及抗氧化酶的影响。结果表明,蓝光下叶片中TBARS(硫代巴比妥酸)含量最高,较对照高30.18%,与希夫试剂染色结果相一致;过氧化氢产生最多,较对照高190.21%,与DAB染色结果一致;超氧阴离子产生速率在蓝光下最高,为对照组的1.54倍,白光下最低;蓝、绿光下脯氨酸含量是对照的2倍;蓝光和黄光处理下,黄酮含量较对照提高了21.4%。蓝、绿光提高了SOD2同工酶的表达,且在蓝光下最高;蓝、绿、红、黄光处理分别诱导了更多的POD基因位点的表达;不同LED光质均提高了CAT抗氧化酶活性,其中蓝、绿光下CAT同工酶的表达量明显高于对照。综上所述,蓝色光处理可以有效提高幼苗的抗氧化能力,可为鱼腥草品质的控制提供参考。     

西双版纳南览河流域常见观赏蝶种及其寄主植物资源调查

西双版纳勐海县境内南览河流域具有良好的天然植被,蝴蝶种类繁多。为了开展重要观赏蝶种的人工繁育试验,2013—2018年对南览河中低海拔地带的常见观赏蝴蝶及其野生寄主植物资源开展了进一步的深入调查,结果表明,经过5年定点调查和沿线观察,在区域内发现兼具较高观赏价值及人工繁育潜力的蝶种46个;通过观察野外成虫的产卵活动,结合野外套袋及室内饲养幼虫,发现蝴蝶寄主植物38种,初步查明了相关蝶种的野生寄主植物资源。调查工作可为筛选出当地适生优良寄主植物、进一步开展相关蝶种的人工养殖试验提供参考。     

草莓白化相关病毒中国分离物全基因组分析

草莓白化相关病毒(strawberrypallidosis-associatedvirus,SPa V)属于长线形病毒科(Closteroviridae)毛形病毒属(Crinivirus),可引起草莓病害,2017年在中国首次报道。采用高通量测序、RACE和RT-PCR技术获得了SPa V中国分离物(FJ)的基因组全长。该病毒含有两条正单链基因组RNA1和RNA2。RNA1全长8 048 nt,5′和3′非编码区序列分别为264和197 nt,含有3个开放阅读框(ORF),分别编码ORF 1a/1b融合蛋白和p9蛋白。RNA2全长7 977 nt,5′和3′非编码区序列分别为248和186 nt,含有8个开放阅读框(ORF),分别编码HSP70h、CPh、CP、CPm、p7、p6、p9和p28等8个蛋白。RNA1和RNA2与美国M1分离物分别具有98.5%和99.0%的核苷酸一致性;系统发育分析结果表明,SPa V中国分离物(FJ)单独处在一个分支。对SPa V来源的小RNA的分析表明,来源于SPa V的小RAN长度以21和22 nt为主。     

甲壳素对连作平邑甜茶生长、光合及抗氧化酶的影响

以苹果连作土盆栽的平邑甜茶幼苗为试材,探讨了0、0.5、1.0和2.5 g·kg-1不同施入量的甲壳素对其光合速率、活性氧含量及抗氧化酶活性的影响。结果表明,1.0 g·kg-1的甲壳素处理能显著促进幼苗株高、地径,干样质量和根冠比的增加,根冠比为对照的1.51倍;明显提高了幼苗叶片光合色素含量、净光合速率和蒸腾速率,其中光合速率为对照的1.30倍;同时提高了幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,分别为对照的1.10倍、1.85倍、1.77倍和1.43倍;减少了叶片丙二醛(MDA)、过氧化氢(H2O2)和超氧阴离子的积累,分别为对照的73%、62%和34%,降低了脯氨酸(Pro)和可溶性糖含量。当甲壳素施用量为2.5 g·kg-1时则显著抑制平邑甜茶幼苗生长,降低幼苗叶片光合速率和抗氧化酶活性,并使超氧阴离子和脯氨酸含量明显上升。因此,适宜用量的甲壳素能减轻苹果的连作障碍。     

一种国外野生沉香的生物活性和化学成分研究

研究一种国外野生沉香乙醚提取物的生物活性并分析其化学成分，为国外野生沉香的进一步研究和开发利用 提供一定的基础。采用乙醚浸提法提取沉香，对提取物分别用 MTT 法、Ellman 法、pNPG 法和贝曼漏斗法进行细胞毒、 乙酰胆碱酯酶抑制、α-葡萄糖苷酶抑制和杀全齿复活线虫活性测试，利用 GC-MS 分析提取物化学成分。结果表明，该 提取物对 5 株人体肿瘤细胞、乙酰胆碱酯酶、α-葡萄糖苷酶和全齿复活线虫均表现出一定的生物活性。GC-MS 检测鉴 定了其中的 26 个化合物，相对含量为 57.39%，包括 23 个倍半萜类成分，相对含量为 56.72%，1 个 2-(2-苯乙基)色酮 类成分，相对含量为 0.35%。本次对此种国外野生沉香的生物活性和化学成分研究，发现沉香乙醚提取物具有杀线虫 的活性，为更好地开发沉香的药用价值提供了一定的科学依据。     

A new species of Malus in China,Malus shizongensis Liu sp. nov

Based on morphological, molecular biological, and molecular systematic studies, we describe here a new species of Malus from Yunnan, China. We compared the morphology of this new species, Malus shizongensis Liu sp. nov, with three Malus species, including M. hupehensis, M. baccata, and M. micromalus. Although the appearance of M. shizongensis was similar to these three species, it differed in height, branch color, branch hair, and flower color. To better identify the taxonomy of this new species, genome of M. shizongensis and that of seven Malus species, including M. prunifolia, M. sylvestris, M. sieversii, M. hupehensis, M. baccata, M. robusta, and M. micromalus were analyzed. A phylogenetic tree based on genome analysis indicated that M. shizongensis was close to M. hupehensis. Furthermore, M. shizongensis had its species-specific SNPs, and the number of species-specific SNPs was similar to that of three close species(M. hupehensis, M. baccata, and M. micromalus). Based on the above information, we named this new species as M. shizongensis Liu sp. nov.     

Mycoplasma pneumoniae induces IL-1β production through activating NLRP3 inflammasome by ROS in RAW264.7 cells

AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells.     

极小种群野生植物八蕊单室茱萸的种群状况研究

八蕊单室茱萸为云南省特有的极小种群野生植物和濒危物种。通过查阅相关文献并结合访问调查,确定八蕊单室茱萸可能分布的区域,开展种群资源调查。结果显示:八蕊单室茱萸目前仅在普洱市和西双版纳州有10个天然种群,其种群规模已低于最小可存活种群,属极小种群野生植物和濒危物种,亟需开展保护工作。生境破坏和生境片段化是导致八蕊单室茱单室茱萸濒危的人为因素。提出收集种质资源、营建和管护近地保护种群、建立迁地和回归种群等保育建议。