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71.
Yuuri Hirooka Takao Kobayashi Jun Takeuchi Tsuyoshi Ono Yasunori Ono Keiko T. Natsuaki 《Journal of General Plant Pathology》2007,73(5):330-335
A ring spot disease of Aloe vera was found on leaves of potted seedlings of Aloe vera in Hachijojima and Chichijima Islands, Tokyo. From tissue of ring spot lesions, a fungus producing Fusarium-type conidia was consistently isolated. After 1 month, reddish perithecia of nectriaceous fungus had formed on the colonies
of this isolate on PDA. These nectriaceous and Fusarium fungi were identified as Haematonectria haematococca and Fusarium sp., respectively. From a single ascospore isolation, the former was confirmed to be the teleomorph of the Fusarium sp. Typical ring spot lesions were reproduced by artificial inoculations using single ascospore and single conidium isolates.
Inoculations of five species of genus Aloe revealed that they were highly susceptible except for A. arborescens. This is the first report of a disease on Aloe caused by H. haematococca (anamorph: Fusarium sp.) in Japan, and it was named aloe ring spot. 相似文献
72.
The employment of formulateBacillus subtilis as a biocontrol agent successfully controlledFusarium oxysporum f.sp.lycopersici within tomato seedlings (in vivo). B. subtilis was able to proted cortex and vascular tissues of tomato against progression of the wilt pathogen. No changes were observed
in tomato tissues due to application ofB. subtilis except for hypertrophy and elongation of cortex tissues, which indicates the production of plant growth hormones byB. subtilis. 相似文献
73.
抗香蕉枯萎病菌拮抗菌的鉴定及其定殖 总被引:4,自引:0,他引:4
通过逐步提高药物浓度方法,获得了抗利福平400μg/ml、对香蕉枯萎病菌拮抗活性稳定的T2WF和W10标记菌株。初步确定两菌株为芽孢杆菌属细菌。采用灌根接种法,在香蕉苗根际土和香蕉苗体内定殖结果表明,拮抗菌液接种浓度为5×104cfu/ml,两菌株均可从香蕉根际土和香蕉体内分离到,并在香蕉体内定殖和传导。在香蕉根际土、根部、球茎和假茎中,T2WF标记菌的菌量高峰分别出现在接种后5、5、11和3d,分别为707、437、273、117cfu/mg;而W10标记菌的菌量高峰分别出现在接种后5、3、5和5d,分别为784、260、253、110cfu/mg。两菌株在香蕉根际土和香蕉体内1~15d的消长动态均有一个共同的“由升到降”趋势。从数量和数量变动幅度上看,香蕉根部的菌量明显高于茎部的菌量。 相似文献
74.
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76.
Takato Nakayama Mitsuo Horita Tadayuki Shimanuki 《Journal of General Plant Pathology》2007,73(4):229-234
We investigated soil contamination by Spongospora subterranea f. sp. subterranea (Sss) and disease severity of powdery scab in 29 potato fields in Hokkaido, Japan, using a hydroponic culture method with
tomato seedlings as bait plants. The quantity of Sss infection on the roots of bait plants was evaluated using the polymerase
chain reaction (PCR) and expressed in terms of the infection potential in the soil. The infection potential was positively
correlated with the disease severity of harvested tubers, whereas the spore ball density determined using PCR had an indistinct
relationship with disease severity. The infection potential can be useful in evaluating soil contamination and in applying
countermeasures against powdery scab. 相似文献
77.
78.
灵芝子实体及其深层培养产物特性的研究 总被引:16,自引:0,他引:16
本文对灵芝(G—6)子实体与发酵菌丝体的粗多糖、多糖组份、粗蛋白及氨基酸组成进行了比较。结果表明:发酵菌丝体中含有子实体中所具备的组份,但各组份在量上不同。菌丝体中的粗多糖及多糖含量均高于子实体分别为子实体的2.26倍、3.5倍。菌丝体的蛋白含量明显高于子实体,比子实体高2.47倍,且两者氨基酸组成不一致。其中子实体中必需氨基酸为总氨基酸量的58.4%,而菌丝体中必需氨基酸为总氨基酸的45.2%。 相似文献
79.
G.M.L.W. Kroes E. Sommers W. Lange 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):561-568
Two types of in vitro seedling tests were developed to evaluate resistance in flax (Linum usitatissimum) against Fusarium oxysporum f.sp. lini. In the first test a solid medium was used. The second test was based on a liquid medium. Disease severity was assessed after three weeks, using the observed reduction of plant length as a scale. Both methods proved to be useful for screening for resistance, for evaluating race specificity of resistance and for studying symptomatology. The solid medium method proved to be the more accurate for the screening, but the liquid medium method was much less time- and labour-consuming. The results of the tests were significantly correlated with observations under field conditions. 相似文献
80.
小麦条锈菌吸器分离方法研究 总被引:1,自引:0,他引:1
[目的]从小麦条锈菌侵染的叶片中分离纯度较高且结构较完整的吸器.[方法]根据锈菌吸器表面含有可以与伴刀豆球蛋白凝集素(Concanavalin A)结合的糖类物质的特性,参照蚕豆锈菌吸器的分离方法对小麦条锈菌吸器进行了分离.[结果]从被条锈菌侵染小麦叶片中成功分离出条锈菌吸器,提取吸器的纯度为83.3%,提取率为5.0×105个/g小麦叶片鲜重.[结论]根据蚕豆锈菌吸器的分离方法,稍作改进,成功分离得到小麦条锈茵吸器,为小麦条锈菌功能基因组学及其致病机制的研究奠定了基础. 相似文献