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91.
银条茎尖培养快繁及离体根状茎的诱导 总被引:2,自引:3,他引:2
以银条‘二细一粗’品种为试材, 研究了蔗糖浓度、激素组合对茎尖培养和快速繁殖的影响及温度、光照、蔗糖浓度等因素对根状茎离体诱导的影响。结果表明: 茎尖培养较理想的培养基为MS + 蔗糖4 % + 6-BA 0.5 mg·L - 1 + NAA 0.1 mg·L - 1 , 成苗率可达74.0 %。茎尖增殖较适培养基为MS + 6-BA 3.0 mg·L - 1 + NAA 0.5 mg·L - 1 , 每个外植体平均可产生5.6 个芽。根状茎诱导以MS + 蔗糖10 % + 6-BA 510 mg·L - 1+ CCC 500 mg·L - 1培养基, 20 ℃全黑暗培养效果最好。 相似文献
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94.
稀土元素铈对‘红地球’葡萄组培苗生长的影响 总被引:3,自引:0,他引:3
以‘红地球’葡萄组培苗茎段为外植体,在培养基中添加1~100 mg·L-1 Ce(NO3)3,,研究 不同浓度Ce(NO3)3对其生长的影响。结果表明适宜浓度的Ce(NO,),(1~10 mg·L-1 )对‘红地球’葡萄组培苗的生长具有促进作用,加快外植体生根,侧根数及侧根总长显著增加,根、茎、叶鲜样质量和干样质量也增加,根系活力提高而增加移栽成活率,增加叶绿素含量,有利于植株对矿质营养的吸收。而100 mg·L-1。Ce(NO3)3对葡萄组培苗生长起明显抑制作用。 相似文献
95.
‘罗田甜柿’胚乳培养获得十二倍体再生植株 总被引:6,自引:1,他引:6
取‘罗田甜柿’自然授粉后约70 d 的胚乳, 采用不同细胞分裂素和生长素组合进行离体培养。结果表明: 以1/ 2MS (1/ 2N ) + ZT (玉米素) 1.1 mg/ L + 2,4-D 1.0 mg/ L + CH (水解酪蛋白) 1000mg/ L 诱导产生的愈伤组织易获得再生植株; 形态学和细胞学检测确认再生植株中存在2n=12x=180 的个体。该十二倍体植株对中国原产甜柿的遗传改良可能具有潜在的应用价值。 相似文献
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西狭文化旅游形象三重结构整合 总被引:2,自引:0,他引:2
李文兵 《干旱区资源与环境》2004,18(3):116-120
西狭文化旅游资源是甘肃成县旅游开发的优势资源。由于对西狭文化理解上的不全面 ,导致西狭文化旅游区的建设存在一系列偏差 ,旅游形象不突出。文章提出了西狭文化旅游形象的三重结构整合的思路 ,即文化结构整合、空间结构整合与景观结构整合 ;并且认为 :文化结构整合是西狭文化旅游形象三重结构整合的前提、基础 ;空间结构整合是由文化结构整合向景观结构整合的过渡 ,而景观结构整合是西狭文化旅游形象三重结构整合的关键。 相似文献
98.
Leaf explants of Caladium ‘Pink Cloud’ were cultured in vitro on MS medium containing various auxins (NAA, IBA, IAA, 2,4,5-T and 2,4-D) in combination with cytokinin (BA). NAA gave the most vigorous in vitro propagation of this plant, and only 15% of the plants were leaf-colour variants on the medium containing 0.5 μmol NAA. Leaf colour variation was observed in all plants regenerated on the medium containing 2,4-D at 0.5–4.5 μmol. In hormone-free medium, only a few leaf-colour variants (6%) occurred, but the rate of plant regeneration was very low. Application of 0.5 μmol NAA together with 4.5 μmol BA seemed to be the most appropriate for in vitro propagation of Caladium ‘Pink Cloud’ with only a few leaf-colour variants. 相似文献
99.
In order to optimise production of the edible flower buds produced by myoga (Zingiber mioga Roscoe) a greater understanding of the growth and development of the plant and factors influencing flower initiation and development are required. The vegetative growth phase in myoga was characterised by a distinct period of pseudostem development from the planted rhizome piece followed by an extended period of rhizome growth from both the base of pseudostems and the planted rhizome piece. The transition from pseudostem formation to the initiation of rhizome growth occurred at the same time as the dry weight of the planted rhizome piece ceased to decline. Flower bud initiation and development occurred over an extended period, beginning soon after the commencement of new rhizome growth and ending prior to foliage senescence. Flower buds were observed at the terminal meristem of first, second, third and fourth order rhizomes. Increasing temperatures stimulated both increased vegetative growth and flower bud initiation and development. Low flower bud yields recorded under conditions of low temperature were the result of reduced rate of floral initiation and not abortion of flower buds. Increased shading of plants grown under glasshouse conditions resulted in reduced flower bud yield and similarly the response resulted from decreased initiation rather than abortion. 相似文献
100.
Clone identification and genetic relationship among vine cacti from the genera Hylocereus and Selenicereus based on RAPD analysis 总被引:1,自引:0,他引:1
Clones of Hylocereus and of Selenicereus species were distinguished from each other by the banding pattern generated by one to nine 10-mer oligonucleotide primers in the random amplified polymorphic DNA (RAPD) reaction. RAPD analysis was also applied to estimate the genetic relationship among five Hylocereus and nine Selenicereus species. A dendrogram was constructed based on a data matrix of 173 polymorphic bands originated by nine primers. Two groups were identified, one consisting of Hylocereus species and the other consisting of Selenicereus species. These results are consistent with the accepted taxonomic classification of the genera studied. The principal coordinate analysis (PCO), i.e. the plot drawn on the basis of the RAPD data, clearly distinguished between three groups, namely, Hylocereus species, S. megalanthus and the rest of the Selenicereus species studied. PCO thus strongly support the notion that the tetraploid S. megalanthus is an exception among the Selenicereus group. The RAPD results support our hypothesis regarding the allopolyploid (rather than autopolyploid) origin of this species. 相似文献