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71.
低蛋白质氨基酸平衡饲粮对早期断奶仔猪腹泻和生产性能的影响 总被引:8,自引:2,他引:6
《中国畜牧杂志》1995,(4):11-13
本研究选用18头28±1日龄断奶的杂交仔猪(平均体重约5.5kg),研究低蛋白质氨基酸平衡饲粮对早期断奶仔猪腹泻和生产性能的影响。试验结果显示:(1)低蛋白质氨基酸平衡饲粮使仔猪血浆挥发性盐基氨含量、血浆尿素氨含量和腹泻指数降低(P<0.05或0.01)。(2)采食全植物蛋白型氨基酸平衡饲粮(CP18.3%)的仔猪,其平均日增重(ADG)、平均日采食量(ADFI)和料肉比(P/G)与采食复合蛋白型对照饲粮(CP19.7%)的仔猪差异不显著(P>0.05),但前者的增重成本比后者降低(P<0.05)31%。(3)采食复合蛋白型氨基酸平衡饲粮(CP18.4%)的仔猪的生产性能优于采食复合蛋白型对照饲粮(CP19.7%)的仔猪,前者的ADG和ADFI分别提高(P<0.05)61%和32%,F/G和增重成本分别下降(P<0.05)24%和37%。(4)采食全植物蛋白型氨基酸平衡饲粮的仔猪,其ADG和ADFI低于(P<0.01)采食复合蛋白型氨基酸平衡饲粮的仔猪。前者的F/G和增重成本趋于高于后者,但差异不显著(P>0.05)。以上结果表明,低蛋白质氨基酸平衡饲粮可显著降低仔猪断奶后腹泻和提高仔猪生产性能。复合蛋白型? 相似文献
72.
经沼液浸种的早稻秧苗比对照组秧苗,具有较强的耐冷性,通过电镜对叶肉细胞超微结构动态观察证实,在低温(5°/5℃)下,叶绿体结构和细胞核结构,随着冷害天数的延长,伤害在不断加重,叶绿体结构会出现空洞,基质片断裂,直至崩溃。细胞核染色体凝集,核膜破裂,核内容物丧失等现象出现。而经沼液浸种的秧苗伤害程度有较明显的减轻。 相似文献
73.
0.05MpH9.6碳酸盐缓冲液和0.05MPH13NaOH溶液作为包被液,分别用于间接ELISA检测乙型五号病病毒抗体。结果表明,用0.05MPH13NaOH溶泡包被可完全灭活五号病病毒抗原而用0.05MPH9.6碳酸盐缓冲液却有87%的五号病病毒抗原未被杀灭;用于检测时,以0.05MPH13NaOH溶液为包被液在敏感性及检测结果的梯度方面都优于用0.05MPH9.6碳酸盐缓冲液。 相似文献
74.
首次应用XXG-A型心血管功能测试仪以无创伤检测技术对16例妊娠黄牛22项血液动力流变学指标做了检测,并首次对怀孕黄牛的血液动力流变学特征做了报道。 相似文献
75.
N. J. M. Roozen J. W. L. Van Vuurde 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):321-334
Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 108 colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×103 and 2.3×104 cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R
2=0.74). 相似文献
76.
用组织培养技术将富士苹果茎尖外植体建立在MS培养基中,形成试管苗。取4—6周龄的茎与愈伤组织,徒手切取约1mm厚的横切面,印迹在硝化纤维膜上。印迹组织经封闭后,与CLSV碱性磷酸酶标抗体反应。对反应结果进行显色。感染CLSV的印迹组织呈紫色反应,正常组织无显色反应。结果表明,该方法十分容易检测印迹组织中的CLSV。带毒愈伤组织较茎的显色反应敏感,显色反应时间仅为茎的一半(15min)。CLSV在愈伤组织中有两种分布情况:一是所有组织均有显色反应;二是呈不均匀分布。CLSV在茎组织中有三种分布情况:第一,除髓部外,表皮、皮层及维管系统中均有分布;第二,不均匀分布,CLSV集中分布于表度组织中,在皮层及韧皮部仅有少量分布;第三,“嵌合”分布,即同一种组织中部分组织带毒,部分为正常组织。 相似文献
77.
78.
Felice LJ Dombrovskis D Lafond E Bartges J Osborne CA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(3):86-89
A reproducible high performance liquid chromatography (HPLC) method was developed for analysis of uric acid in canine serum and urine. The method consists of precipitating serum proteins with phosphotungstic acid prior to HPLC analysis. Urine is analyzed after dilution with buffer. Chromatography is performed on a reversed-phase C-18 column with UV detection at 292 nm. Sensitivity of the method will allow reproducible measurement of uric acid at concentrations of 0.05 mg/dl in serum and 0.1 mg/dl in urine. The HPLC method has been used to quantify hundreds of canine serum and urine samples. The method is superior to UV absorption or colorimetric methods because its lower limit of detection allows measurement of uric acid at concentrations found in canine serum and urine. 相似文献
79.
试验分别用温汤去雄杂交和两种化学去雄剂化学去雄诱导草地早熟禾雄性不育,结果表明,在两种化学去 雄剂作用下去雄效果比较理想,温汤去雄雄性不育率达100%。 相似文献
80.
Forty Swiss Large White piglets of 4 weeks of age were used to determine the effect of dietary Chinese rhubarb on growth performance, as well as on energy and nitrogen metabolism by means of indirect respiration calorimetry studies. A cereal-based diet, supplemented with four different dosages of Chinese rhubarb (0.0%, 0.25%, 0.5%, 1%), was offered ad libitum to the piglets. Gaseous exchange was measured, and urine, faeces, and blood samples were taken. The addition of 0.25% dietary rhubarb increased feed intake (+32%) and daily weight gain (+67%) compared to the diet containing the greatest amount of rhubarb (1%). The piglets fed diet supplemented with 0.25% rhubarb digested and metabolised more energy and nitrogen relative to the animals of treatment 1%. As a consequence, the same group (0.25%) converted more energy and nitrogen into body protein than piglets receiving diet supplemented with 1% rhubarb. However, compared to the control group, the addition of 0.25% rhubarb to the diet resulted in not significantly alter growth performance, energy, and nitrogen metabolism of the animals.Blood parameters were not affected by the rhubarb supplementation. The dose-responding effect of Chinese rhubarb was reflected by the dry matter content of faecal samples. According to the laxative effect of higher doses of rhubarb, the addition of 1% rhubarb to the diet reduced dry matter content of faeces. This effect was well visible in practice, although not relevant in statistical terms. The addition of 0.5% dietary rhubarb had no relevant effect on growth performance or on energy and nitrogen metabolism of treated animals. 相似文献