中国山羊品种资源及其遗传多样性研究

本文论述了中国山羊的起源、品种遗传资源及其遗传多样性的研究进展。最新调查 显示中国的山羊品种达50个,其中43个为地方品种,4个培育品种,3个引入品种,许多品种 具有独特的生产性能或适应能力。大量研究表明,我国山羊遗传多样性较为丰富。     

不同覆盖方式对旱地棉田土壤环境及棉花产量的影响

分析普通地膜覆盖、小麦秸秆覆盖、地膜 秸秆覆盖3种覆盖物对旱地棉田土壤环境、棉花产量构成因素的影响,结果表明:覆盖栽培能改善土壤环境,降低土壤容重,提高土壤水分利用率,调节土壤温、湿度,协调水热资源利用的同步性;秸秆覆盖能增加土壤养分含量,特别是速效钾含量;在棉花生长后期,提高叶面积指数,延长叶片功能期,提高棉株的光合能力从而防止棉花早衰,增加铃重,提高棉花产量。     

西北干旱灾害及其气候趋势研究

利用西北五省1950～2000年降水、温度和农作物旱灾面积资料,用降水的Z指标法,农作物受旱成灾率确定了西北干旱指标和旱灾等级,分析了西北旱灾特征及气候变化趋势。表明西北农业旱灾以70、90年代最严重,而且呈增加趋势,气温在升高,降水在减少。根据预测21世纪前十年西北干旱有所减轻。     

中国北方干旱半干旱地区宏观地貌的发育特征

分析了中国北方地区宏观地貌的基本特征、地质构造背景和地貌发育的主要影响因素,以期形成对北方干旱半干旱地区的自然地理条件、生态环境特征及农业发展基础条件的深刻认识,为生态环境的保护和区域农业规划提供参考。     

Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR

Colletotrichum coccodes is the causal agent of the potato blemish disease black dot. Two PCR primer sets were designed to sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a nested PCR. The genus-specific outer primers (Cc1F1/Cc2R1) were designed to regions common to Colletotrichum spp., and the species-specific nested primers (Cc1NF1/Cc2NR1) were designed to sequences unique to C . coccodes . The primer sets amplified single products of 447 bp (Cc1F1/Cc2R1) and 349 bp (Cc1NF1/Cc2NR1) with DNA extracted from 33 European and North American isolates of C. coccodes. The specificity of primers Cc1NF1/Cc2NR1 was confirmed by the absence of amplified product with DNA of other species representing the six phylogenetic groups of the genus Colletotrichum and 46 other eukaryotic and prokaryotic plant pathogenic species. A rapid procedure for the direct extraction of DNA from soil and potato tubers was used to verify the PCR assay for detecting C. coccodes in environmental samples. The limit of sensitivity of PCR for the specific detection of C. coccodes when inoculum was added to soils was 3·0 spores per g, or the equivalent of 0·06 microsclerotia per g soil, the lowest level of inoculum tested. Colletotrichum coccodes was also detected by PCR in naturally infested soil and from both potato peel and peel extract from infected and apparently healthy tubers. Specific primers and a TaqMan fluorogenic probe were designed to perform quantitative real-time (TaqMan) PCR to obtain the same levels of sensitivity for detection of C. coccodes in soil and tubers during a first-round PCR as with conventional nested PCR and gel electrophoresis. This rapid and quantitative PCR diagnostic assay allows an accurate estimation of tuber and soil contamination by C. coccodes .     

Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding of common bean

The genetic and virulence variability of 112 isolates of Phaeoisariopsis griseola , collected from various locations in Central America, were studied using seven random amplified polymorphic DNA (RAPD) primers and 12 common-bean differential genotypes. Broad molecular diversity ( H  = 0·92) among isolates was found using RAPD markers. Fifty pathotypes were identified on 12 differential bean genotypes, 29 of which were represented by only one isolate. Only 18 pathotypes were found in two or more countries. Pathotype 63-63 was the most virulent and caused leaf spots on all 12 common-bean differential genotypes. Comparison of virulence phenotypes and RAPD profiles to known Andean P. griseola isolates confirmed that all isolates belonged to the Mesoamerican group. Pairwise comparison between individual RAPD loci showed that the majority were in gametic phase linkage disequilibrium, revealing that P. griseola maintains a genetic structure that is consistent with asexual reproduction. The molecular and virulence diversities of P. griseola isolates from Central America imply that using single resistance genes to manage angular leaf spot is inadequate and stacking resistance genes may be necessary to manage the disease effectively.     

黑河流域荒漠地区梭梭人工林地土壤水分动态研究

梭梭林地土壤剖面的含水量与裸沙地含水量显著不同 ,裸沙地土壤含水量随深度增加而增加 ,林地在 30～ 12 0cm范围内有较高的含水量 ;林地 0～ 30cm的上层土壤含水量受降雨及蒸发的剧烈影响 ,30～ 2 0 0cm范围内的林地土壤各测量层平均含水量与其标准差有二次函数的关系 ;降雨对每月的 0～ 2 0 0cm的土壤贮水量没有明显的影响 ,说明降雨多数在当月就被蒸散和深层渗漏所消耗 ;认为梭梭人工林这种脆弱生态系统能够在严酷的环境条件下存在 ,其根系对土壤水和地下水有吸水和释水的再分配功能是重要的因素之一     

土壤湿度对三氮苯类除草剂药效的影响及优化方案

以稗草为生物测定材料,运用二次正交旋转组合设计,以土壤湿度和除草剂用量二因子为决策变量,对稗草的抑制率为目标函数,研究土壤湿度对三氮苯类除草剂药效的影响。结果表明,适当的土壤水分是三氮苯类除草剂发挥药效的重要因素,药效随土壤湿度的提高而提高。不同的土壤湿度对不同除草剂药效影响各异,高湿条件下,湿度差异对药效影响大小依次为嗪草酮、西草净、扑草净、莠去津,低湿条件下则相反。除草剂用量与土壤湿度存在最佳发挥药效的组合。     

转Bt基因棉在安庆市大面积种植后害虫发生特点调查

根据调查,转Bt基因棉大面积种植后,棉铃虫、红铃虫发生减轻;棉叶螨发生加重,为害时间延长;甜菜夜蛾、斜纹夜蛾发生面积扩大,为害程度加重。据此初步提出了当地转Bt基因棉害虫防治中应注意的问题和管理对策。