Long-term fertilization effects on active ammonia oxidizers in an acidic upland soil in China

The effects of long-term fertilization of acidic soils on ammonia-oxidizing archaea (AOA) and bacteria (AOB) communities and its ecological implications remain poorly understood. We chose an acidic upland soil site under long-term (27-year) fertilization to investigate ammonia oxidizer communities under four different regimes: mineral N fertilizer (N), mineral NPK fertilizer (NPK), organic manure (OM) and an unfertilized control (CK). Soil net nitrification rates were significantly higher in OM soils than in CK, N or NPK soils. Quantitative analysis of the distribution of amoA genes by DNA-based stable isotope probing revealed that AOA dominate in CK, N and NPK soils, while AOB dominate in OM soils. Denaturing gradient gel electrophoresis and clone library analyses of amoA genes revealed that Group 1.1a-associated AOA (also referred to as Nitrosotalea) were the most dominant active AOA population (>92%), while Nitrosospira Cluster 3 and Cluster 9 were predominant among active AOB communities. The functional diversity of active ammonia oxidizers in acidic soils is affected by long-term fertilization practices, and the responses of active ammonia oxidizers to mineral fertilizer and organic manure are clearly different. Our results provide strong evidence that AOA are more highly adapted to growth at low pH and low substrate availability than AOB, and they suggest that the niche differentiation and metabolic diversity of ammonia oxidizers in acidic soils are more complex than previously thought.     

Can soil moisture deficit be used to forecast when soils are at high risk of damage owing to grazing animals?

A potentially significant cause of damage to grassland soils is compaction of unsaturated soil and poaching of saturated or nearly saturated soil by animal hooves. Damage is caused when an applied stress is in excess of the bearing strength of the soil and results in a loss of soil structure, macroporosity and air or water conductivity. Severely damaged soils can cause reduced grassland productivity and make grazing management very difficult for the farmer. The actual amount of soil damage that can occur during grazing is dependent on the grass cover which acts as a protecting layer, the soil water content and the characteristics of the grazing animal (weight and hoof size). Assuming that the farmer is knowledgeable about the characteristics of the grazing animal and grass cover, it would be very useful for short‐term operational farm planning to be able to predict when soil water contents were likely to be in a critical range with respect to potential hoof damage. In this study soil moisture deficits (SMDs) which can be derived from meteorological forecasts are evaluated for predicting when soil water conditions are likely to lead to hoof damage. Two contrasting Irish grassland soils were analysed using a Hounsfield servo‐mechanical vertical testing machine to simulate static (285.4 N) and dynamic (571 N) hoof loads on the soil over a range of estimated SMDs (0, 5, 10 and 20 mm). The deficits were analysed with respect to the soil volumetric water content, compression (displacement) and change in dry bulk density. The SMDs imposed in the laboratory were similar to those under field conditions and thus the methods used in this study are applicable elsewhere. The change in dry bulk density following loading (0.2–0.7 g/cm³) was linearly related to SMD (R² ranged from 0.90 to 0.99), leading to the conclusion that a forecast of SMD can be used to predict when grassland soils are likely to be at risk of damage from grazing.     

优化施肥对春小麦产量、氮素利用及氮平衡的影响

2009 ～ 2010年,在宁夏引黄灌区分别以宁春11号和宁春16号小麦为供试作物,利用田间试验研究了优化施肥(OPT)和习惯施肥(CON)对春小麦产量、氮素吸收利用和土壤硝态氮累积的影响,表观评估了土壤—小麦体系氮素平衡.结果表明,相对于CK处理,OPT和CON都显著提高春小麦籽粒产量地上部生物量,并促进籽粒N和地上...     

广西5种人工林地土壤微生物数量及土壤酶活性分析

为当地珍贵速生树种人工林的发展及合理利用提供理论依据。分析广西5种珍贵树种人工林土壤微生物数量及土壤酶活性差异,以相同立地条件下12年生的黑木相思(Acacia melanoxylon)、老排(Mytilaria laosensis)、红椎(Castanopsis hystrix)、黧蒴椎(Castanopsis fissa)和火力楠(Michelia macelurei)人工纯林为研究对象,采用稀释平板涂布法和土壤酶活性测定法,测定和分析该5种人工林地土壤微生物数量及酶活性。结果表明:5种林地的土壤微生物总数和细菌数量大小均为:火力楠黧蒴锥红椎黑木相思米老排。但5种林地的土壤酶活性大小不一,蔗糖酶、脲酶、蛋白酶、酸性磷酸酶活性在5种林地之间的差异极其显著,而过氧化氢酶活性不显著。5种林地中土壤酶活性相对较强的是米老排人工林,较弱的是黑木相思林和火力楠林。土壤酶活性与微生物数量的相关分析表明,土壤中蔗糖酶活性与放线菌数量呈极显著正相关关系,其余相关性没有达到显著水平。     

四川攀西山区植烟土壤养分特征分析与肥力评价

了解土壤肥力对烟田土壤保育具有积极的指导意义。本研究采集了四川攀西山区4个代表性植烟县(区)的19个典型烟田耕层样品,测定了对烤烟产质量影响大且能反映土壤肥力的指标,利用模糊综合评价法计算了土壤肥力综合肥力指数(IFI)。结果表明：19个烟田耕层土壤的pH、有机质、全氮、碱解氮、有效磷、有效钾和水溶性氯含量平均值分别为6.25、21.6 g/kg、1.28 g/kg、120.61 mg/kg、42.84 mg/kg、148.69 mg/kg和7.47 mg/kg,适宜的植烟烟田比例分别为52.67%、26.32%、36.84%、42.11%、36.84%、84.21%和21.05%。烟田土壤肥力综合评价指数介于0.30～0.87,平均为0.55,总体上处于较高水平。施肥上应注意多施钾肥和磷肥并提高其利用率,对氯素低的烟田(<10 mg/kg)可以酌情施用氯肥。     

生长调节物质对小豆豆芽营养成分影响和功能性评价

为筛选最适合芽菜培养的小豆品种,以‘白红8号’为试材,设置3种生长调节物质(乙酰水杨酸、乙烯利和6-苄氨基腺嘌呤(6-BA))的各6个浓度(0.0、0.1、0.2、0.3、0.4、0.5 mmol/L;0、20、30、40、50、60 mg/L和0、2、3、4、5、6 mg/L)喷施处理,测定其生物特性(上胚轴长度、上胚轴直径、萌发率、鲜重、干重和产出比)、营养成分(可溶性蛋白和可溶性糖)及抗氧化等特性。结果表明:3种生长调节物质喷施处理都能够提升其营养价值,在乙烯利喷施浓度为50 mg/L,‘白红8号’小豆芽苗菜中各营养成分、酶活性及抗氧化性等均可达到最大值,显著提高了其营养价值。因此,为提高生产中小豆芽苗菜的产量和营养价值,最终确定乙烯利喷施浓度为50 mg/L效果最佳。     

扎鲁特旗山地草地土壤含水量动态与气候因子的关系

通过对内蒙古通辽市扎鲁特旗巴雅尔吐胡硕牧业气象站1983-2012年气象数据和山地草地土壤含水量数据分析,揭示土壤含水量的动态变化及其与气候因子之间的相关关系。结果表明,1983-2012年山地草地土壤各层(0-10、10-20、20-30、30-40、40-50和0-50cm)含水量均呈现逐渐降低的变化趋势,草地逐渐旱化;土壤含水量下降过程可以分为3个阶段,第1阶段为1983-1987年,该阶段土壤含水量在15%~30%之间波动,属于土壤含水量的较高阶段;第2阶段为1988-1999年,该阶段土壤含水量在5%~15%范围内波动,属于土壤含水量高(15%~30%)向低(2%~10%)过渡阶段;第3阶段是2000-2012年,该阶段土壤含水量在2%~10%范围内波动,属于土壤含水量较低阶段;土壤含水量与温度和日照时数呈负相关,与降水量、相对湿度、蒸散量呈正相关。     

Sewage sludge processing determines its impact on soil microbial community structure and function

Composting and thermal drying are amongst the most commonly used post-digestion processes for allowing sanitation and biological stabilization of sewage sludge from municipal treatment plants, and making it suitable as soil conditioner for use in agriculture. To assess the impact of sludge-derived materials on soil microbial properties, fresh (LAF), composted (LAC) and thermally dried (LAT) sludge fractions, each resulting from a different post-treatment process of a same aerobically digested sewage sludge, were added at 1% (w/w) application rate on two contrasting (a loam and a loamy sand) soils and incubated under laboratory conditions for 28 days. Soil respiration, microbial ATP content, hydrolytic activities and arginine ammonification rate were monitored throughout the incubation period. Results showed that soil biochemical variables, including the metabolic quotient (qCO₂), were markedly stimulated after sludge application, and the magnitude of this stimulatory effect was dependent on sludge type (precisely LAT > LAF > LAC), but not on soil type. This effect was related to the content of stable organic matter, which was lower in LAT. Genetic fingerprinting by PCR–DGGE revealed that compositional shifts of soil bacterial and, at greater extent, actinobacterial communities were responsive to the amendment with a differing sludge fraction. The observed time-dependent changes in the DGGE profiles of amended soils reflected the microbial turnover dependent on the sludge nutrient input, whereas no indications of adverse effects of sludge-borne contaminants were noted. Our findings indicate that composting rather thermal drying can represent a more appropriate post-digestion process to make sewage sludge suitable for use as soil conditioner in agriculture.     

氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响

为探究氮添加对高寒草原生态系统土壤酶活性的影响,于2018年在中国科学院巴音布鲁克草原生态系统研究站,选择4个氮添加水平(对照,N0,0 kg·hm^-2·a^-1;低氮,N1,10 kg·hm^-2·a^-1;中氮,N3,30 kg·hm^-2·a^-1;高氮,N9,90 kg·hm^-2·a^-1),开展土壤酶活性对氮添加响应的研究,分析土壤酶活性对氮添加的响应特点,土壤酶化学计量比以及土壤酶活性与土壤环境因子的关系。结果表明:与对照相比,氮添加在N3水平显著增加β-1,4葡萄糖苷酶(βG)、β-D-纤维二糖水解酶(CBH)和β-1,4木糖苷酶(βX)酶活性(P<0.05),N1和N3水平显著增加碱性磷酸酶(AKP)活性(P<0.05),N3水平显著降低多酚氧化酶(PPO)活性(P<0.05),氮添加对亮氨酸氨基肽酶(LAP)活性影响不显著,N3水平下显著增加N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性(P<0.05)。相关分析表明,8种土壤酶活性均与土壤有机碳(SOC、NAG除外)和总磷(TP)显著相关,与土壤总氮(TN)不相关。研究区土壤酶活性C∶N∶P化学计量比为1∶1∶1.2,与全球生态系统的土壤酶活性C∶N∶P的比值1∶1∶1相偏离,表明该研究区土壤微生物生长受磷素限制。冗余分析(RDA)进一步揭示出土壤有机碳和土壤全磷含量是影响土壤酶活性的主要因子。     

Contributions of ammonia-oxidizing archaea and bacteria to nitrification in Oregon forest soils

Ammonia oxidation, the first step of nitrification, is mediated by both ammonia-oxidizing archaea (AOA) and bacteria (AOB); however, the relative contributions of AOA and AOB to soil nitrification are not well understood. In this study we used 1-octyne to discriminate between AOA- and AOB-supported nitrification determined both in soil-water slurries and in unsaturated whole soil at field moisture. Soils were collected from stands of red alder (Alnus rubra Bong.) and Douglas-fir (Pseudotsuga menziesii Mirb. Franco) at three sites (Cascade Head, the H.J. Andrews, and McDonald Forest) on acidic soils (pH 3.9–5.7) in Oregon, USA. The abundances of AOA and AOB were measured using quantitative PCR by targeting the amoA gene, which encodes subunit A of ammonia monooxygenase. Total and AOA-specific (octyne-resistant) nitrification activities in soil slurries were significantly higher at Cascade Head (the most acidic soils, pH < 5) than at either the H.J. Andrews or McDonald Forest, and greater in red alder compared with Douglas-fir soils. The fraction of octyne-resistant nitrification varied among sites (21–74%) and was highest at Cascade Head than at the other two locations. Net nitrification rates of whole soil without NH₄⁺ amendment ranged from 0.4 to 3.3 mg N kg⁻¹ soil d⁻¹. Overall, net nitrification rates of whole soil were stimulated 2- to 8-fold by addition of 140 mg NH₄⁺-N kg⁻¹ soil; this was significant for red alder at Cascade Head and the H.J. Andrews. Red alder at Cascade Head was unique in that the majority of NH₄⁺-stimulated nitrifying activity was octyne-resistant (73%). At all other sites, NH₄⁺-stimulated nitrification was octyne-sensitive (68–90%). The octyne-sensitive activity—presumably AOB—was affected more by soil pH whereas the octyne-resistant (AOA) activity was more strongly related to N availability.