Ureolytic Activity of Soybean and Corn Residue Extracts

The enzyme urease is responsible for the rapid hydrolysis of urea in agroecosystems where more than 50% of the applied nitrogen (N) can be lost via ammonia volatilization. The objectives of the study were to (1) extract urease from corn (Zea mays L.) and soybean (Glycine max L.) and (2) compare the urease activity from soybean and corn residues to Jackbean (Canavalia ensiformis) urease using Fourier transform infrared spectroscopy (FTI-R). Concentrations of urea and sodium bicarbonate ranging from 0 to 200 mM were analyzed with FTI-R to determine the correlation to peak height. Bicarbonate produced the most responsive peaks to concentration at 1,362 cm^?1. Urease extracted from soybean residue was active, producing a bicarbonate peak at 1362 cm^?1, whereas no urease activity was observed in corn residue. Variation among urease activities in crop residues could suggest a need for more precise nitrogen management in conservation tillage agroecosystems to reduce ammonia volatilization.     

Nitrogen effects on cantaloupes

Abstract

The NO₃ levels in cantaloupe (Cucumis melo L.) petioles relate closely to N treatment and reflect the N status of the cantaloupe plant. Suggested levels of petiole NO₃ in cantaloupes grown in Arizona are presented. Nitrogen treatment had little effect on melon size, earliness, or most storage quality factors that were evaluated except that deficiencies resulted in lower netting, poor general appearance and an increase in cull melons.     

Volumetric soil measures for routine soil testing

Abstract

The design, dimensiors and materials for constructing volumetric soil measures for routine soil testing use are presented. Scoop calibration techniques are also described. Reproducibility of results obtained under routine laboratory, conditions are shown. The measures include volumes of 1.0‐, 2.5‐, 5.0‐ and 10‐ cm³ respectively.     

喷施硅铈溶胶缓解镉铅对小白菜毒害的研究

通过大田试验,在轻度镉-铅复合污染菜地向小白菜叶面喷施不同浓度的硅溶胶、铈溶胶及硅铈复合溶胶,研究其对产量、品质、抗氧化酶活性及Cd、Pb吸收的影响。结果表明,喷施硅铈溶胶均能促进小白菜生长,增加小白菜生物量和提高维生素C、可溶性糖的含量,降低亚硝酸盐的含量;显著增强抗氧化系统保护酶(SOD、POD)活性和降低小白菜地下部、地上部中镉、铅的含量及累积量。在不同浓度的施硅和施铈处理中,喷施0.50 g kg-1SiO2处理和0.20 g kg-1CeO2处理在增产、提升品质和缓解重金属对小白菜毒害上效果最佳,喷施硅铈复合溶胶虽效果显著,但与单独施硅、施铈相比无明显提高。     

紫色土坡耕地氮素淋失通量的实测与模拟

氮淋失是氮素循环研究中最重要的环节之一,获得准确的氮淋失通量是当今农田氮循环研究中必不可少而又较为困难的工作之一。紫色土土层薄,土壤氮素难以蓄存,加之降水丰富,下伏透水性较弱的母岩,淋溶水达到母岩后难以垂直下渗而沿土壤岩石界面出流、汇流形成壤中流,紫色土氮素淋失主要表现为氮素随壤中流迁移流失。DNDC模型是基于过程的一种土壤碳氮循环模型,常用于农田温室气体排放模拟,但其应用于氮素淋溶的验证与测试不足。本文利用大型坡地排水采集器(lysimeter),测定紫色土坡耕地淋溶水量(壤中流流量)和氮素淋失通量,并利用观测数据对DNDC模型进行验证。结果表明,紫色土坡耕地小麦玉米季累积淋溶水通量为323.6 mm,径流系数33.3%,氮素淋失量为36.93 kg.hm 2,占全年氮素施用量的13.2%。壤中流流量与氮素淋失量实测值和模拟值的Pearson相关系数分别为0.944(P<0.05)和0.972(P<0.05),Theil不等系数分别为0.07和0.1,降雨量、土壤孔隙率和施氮水平是氮流失模拟的高敏感性参数。DNDC模型应用于紫色土坡耕地氮素淋失通量的模拟具有较高的可靠性,同时DNDC基于过程模型的优势可以描述持续降雨条件下的氮淋失过程,未来可通过进一步的验证,测试DNDC模型应用于氮淋失过程及区域氮淋失评估的可行性。     

横坡和顺坡耕作对紫色土土壤团聚体稳定性的影响

通过径流小区试验,分析探讨了紫色土横坡和顺坡两种耕作模式下表层(0~20 cm)土壤水稳性团聚体及有机碳含量的特征,为紫色土区坡耕地的有效改造和综合利用提供科学依据。试验结果表明,横坡耕作下土壤水稳性指数K值比顺坡耕作高38.99%,而土壤分散性D值为顺坡耕作的1.64倍,横坡耕作抗蚀性大于顺坡耕作。横坡耕作>0.25 mm水稳性团聚体含量比顺坡耕作增加3.62%,>5 mm、5~3 mm、3~2 mm水稳性团聚体含量横坡耕作均高于顺坡耕作,横坡耕作显著提高>2 mm水稳性团聚体含量。横坡耕作下团聚体结构体破坏率较顺坡耕作减少3.05%,团聚体平均重量直径为顺坡耕作的1.39倍。2种耕作模式下0.5~2 mm团聚体有机碳含量均最高,>0.25 mm不同粒级团聚体有机碳含量百分数均随粒径的减小而减小,均在0.5~0.25 mm粒级下达到最小。横坡和顺坡耕作模式下2~1 mm团聚体有机碳含量无显著差异。横坡耕作较顺坡耕作能够显著增加紫色土>2 mm水稳性团聚体含量,且对有机碳的固持作用更好,有利于土壤结构的改善。     

太湖地区不同水旱轮作方式下稻季甲烷和氧化亚氮排放研究

为准确编制我国稻田温室气体排放清单及制定合理减排措施提供基础数据,选择太湖地区典型水稻种植区江苏省苏州市,研究设计了休闲水稻(对照,CK)、紫云英水稻(T1)、黑麦草水稻(T2)、小麦水稻(T3)和油菜水稻(T4)5种水旱轮作方式,采用静态箱气相色谱法,开展了不同水旱轮作方式下水稻生长季田间甲烷(CH4)和氧化亚氮(N2O)排放监测试验。试验结果表明:不同水旱轮作方式下水稻生长季CH4排放通量呈先升高后降低的变化趋势,CH4排放峰值出现在水稻生育前期,移栽至有效分蘖临界叶龄期CH4累积排放量占全生育期排放总量的比例为65%~81%,而N2O仅在水稻烤田期间有明显排放。水旱轮作方式对稻季CH4和N2O排放有极显著(P 0.01)影响,CH4季节总排放量表现为T1(283.2 kg.hm 2)CK(139.5 kg.hm 2)T3(123.4kg.hm 2)T4(114.7 kg.hm 2)T2(100.8 kg.hm 2),N2O季节总排放量顺序为T1 T4 T3 T2 CK,依次为1.06kg.hm 2、0.87 kg.hm 2、0.81 kg.hm 2、0.72 kg.hm 2和0.53 kg.hm 2。T1处理稻季排放CH4和N2O产生的增温潜势最高[7 396 kg(CO2).hm 2],显著(P 0.05)高于其他处理,比CK[3 646 kg(CO2).hm 2]增加103%,T2[2 735kg(CO2).hm 2]较CK减少25%(P 0.05)。紫云英水稻轮作方式增加了太湖地区水稻生长季的温室效应。     

蜡样芽孢杆菌菌株X5强化的生物有机肥能有效控制根结线虫(Meloidogyne sp.)

The efficacy of Bacillus cereus X5 as a potential biological control agent against root-knot nematodes was evaluated in vitro by examining second-stage juvenile mortality and egg hatching rate under addition of culture filtrate and in planta by application of bio-organic fertilizers enhanced with B. cereus X5, B. thuringiensis BTG, or Trichoderma harzianum SQR-T037 alone or together in greenhouse and field experiments. The biofumigation of the root-knot nematode-infested soil with organic materials (chicken manure, pig manure and rice straw) alone or in combination with B. cereus X5 was also conducted in greenhouse experiments. In laboratory, the filtrate of B. cereus X5 more effectively reduced egg hatching rates during the incubation period for 14 d and more effectively killed the second-stage juvenile during the incubation period of 24 h than that of B. thuringiensis BTG. The highest dry shoot weights for greenhouse tomatoes and field muskmelons were found in both the treatment consisting of the bio-organic fertilizer enhanced with the three biocontrol agents and the treatment consisting of the bio-organic fertilizer enhanced only with B. cereus X5. The two bio-organic fertilizers achieved better nematicidal effects than those enhanced only with B. thuringiensis BTG or T. harzianum SQR-T037. B. cereus X5 also enhanced effect of biofumigation, which resulted in increased plant biomass and reduced nematode counts in the roots and rhizosphere soil. Therefore, these results suggested that biological control of root-knot nematodes both in greenhouses and fields could be effectively achieved by using B. cereus X5 and agricultural wastes.     

滩涂土壤有机碳空间分布与围垦年限相关性分析

中国具有治理和修复潜力的滨海滩涂盐土面积约为2000hm2。滨海滩涂盐土作为新成土壤,土体发育不明显、理化性状差、肥力水平低下。该文通过对东台境内表层土壤有机碳的采样分析,在ArcGIS地理信息系统及SPSS16.0系统平台上,利用地统计分析模块中的克里格法分析东台市表层土壤有机碳的空间变异规律,并分析不同滩涂围垦年限下表层土壤有机碳、总氮、pH值、EC1:5的变化特征及其相关关系,结果显示滨海表层土壤有机碳含量与总氮、围垦年限呈极显著正相关,相关系数r分别为0.959和0.749,与pH值、电导率EC1:5呈极显著负相关,相关系数r分别为-0.478和-0.649。围垦3a以上表层土壤有机碳可增加0.24%左右,总氮质量分数增加0.02%左右;围垦时间大于52a,有机碳质量分数自0.1%增加到1.6%,全氮质量分数自0.03%增加到0.12%,养分等级也相应从6级增加到3级。该文研究表明,滨海盐土随着围垦年限的增长,土壤碳储量与总氮含量随之增加、pH值与EC1:5随之降低,耕地质量也相应提高。     

激光平地改善土壤水盐分布并提高春小麦产量

为了促进激光平地技术的应用与推广,在河套灌区以普通畦田为对照,分期、分段测定了激光平地畦田0～80 cm土层土壤水分、土壤盐分及春小麦生长状况。结果表明,从渠口到畦尾,激光平地畦田不同地段的土壤水分在春小麦生长的不同时期无显著差异,但从拔节期开始,上层土壤盐分逐渐升高;普通畦田从三叶期开始,上层土壤水分、盐分逐渐提高,且显著或极显著高于激光平地。普通畦田从三叶期开始,激光平地从拔节期开始,从渠口到畦尾,春小麦的生长状况逐渐变差,千粒质量、产量均逐渐降低,但激光平地的春小麦千粒质量、产量高于普通畦田。激光平地改善了畦田的土壤水分、盐分分布,促进了畦田中后段的春小麦生长,提高了畦田中后段的水分利用率及产出率。