全文获取类型
收费全文 | 3391篇 |
免费 | 165篇 |
国内免费 | 316篇 |
专业分类
林业 | 98篇 |
农学 | 380篇 |
基础科学 | 38篇 |
468篇 | |
综合类 | 1247篇 |
农作物 | 273篇 |
水产渔业 | 259篇 |
畜牧兽医 | 784篇 |
园艺 | 121篇 |
植物保护 | 204篇 |
出版年
2024年 | 17篇 |
2023年 | 46篇 |
2022年 | 81篇 |
2021年 | 82篇 |
2020年 | 105篇 |
2019年 | 122篇 |
2018年 | 95篇 |
2017年 | 145篇 |
2016年 | 154篇 |
2015年 | 119篇 |
2014年 | 159篇 |
2013年 | 224篇 |
2012年 | 306篇 |
2011年 | 229篇 |
2010年 | 202篇 |
2009年 | 175篇 |
2008年 | 179篇 |
2007年 | 215篇 |
2006年 | 143篇 |
2005年 | 117篇 |
2004年 | 107篇 |
2003年 | 88篇 |
2002年 | 64篇 |
2001年 | 70篇 |
2000年 | 77篇 |
1999年 | 40篇 |
1998年 | 47篇 |
1997年 | 51篇 |
1996年 | 48篇 |
1995年 | 47篇 |
1994年 | 35篇 |
1993年 | 38篇 |
1992年 | 31篇 |
1991年 | 32篇 |
1990年 | 31篇 |
1989年 | 40篇 |
1988年 | 21篇 |
1987年 | 15篇 |
1986年 | 9篇 |
1985年 | 16篇 |
1984年 | 15篇 |
1983年 | 10篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有3872条查询结果,搜索用时 15 毫秒
91.
Julie M. Barr BS Safdar A. Khan DVM MS PhD DABVT Sheila M. McCullough DVM MS DACVIM Petra A. Volmer DVM MS DABVT DABT 《Journal of Veterinary Emergency and Critical Care》2004,14(3):196-202
Objective: To correlate the sodium chloride dosage and the serum sodium concentration to clinical signs, to determine if the dosage of homemade play dough (and, therefore, sodium chloride) is the most reliable way to predict clinical signs and prognosis, and to review previously reported treatment options. Design: Retrospective case series. Animals: Fourteen dogs with a history of homemade play dough ingestion. Procedure: Cases were examined for each animal's signalment including body weight, age, approximate amount of play dough ingested, the estimated sodium chloride dosage, clinical signs, serum sodium concentration, and outcome when available. The literature was reviewed to determine pathophysiology and treatment regimens. Results: Twelve of 14 dogs (86%) that ingested homemade play dough showed clinical signs. Vomiting (9 of 14, 64%), polydipsia, and seizures (4 of 14 each, 29%) were the most common signs followed by polyuria, tremors (3 of 14 each, 21%), and hyperthermia (2 of 14, 14%). The lowest calculated dosage associated with objective clinical signs was 1.9 g/kg. Seizures were reported in all animals with serum sodium levels greater than 180 mEq/L. Conclusions and clinical relevance: Homemade play dough ingestion can be a serious and life‐threatening problem. Many factors can contribute to the toxicity of homemade play dough. This study indicates that the serum sodium concentration is a more reliable indicator of the clinical course of the toxicity rather than the amount of play dough and, therefore, the dosage of sodium chloride ingested. Treatment should be based on a clinical evaluation of the patient and laboratory results, and consists of controlling seizures, reducing serum sodium concentrations slowly, and supportive care. 相似文献
92.
Canine Haematopoietic Chimerism Analyses by Semiquantitative Fluorescence Detection of Variable Number of Tandem Repeat Polymorphism 总被引:1,自引:0,他引:1
Hilgendorf I Weirich V Zeng L Koppitz E Wegener R Freund Junghanss C 《Veterinary research communications》2005,29(2):103-110
Canine models are successfully applied to the study of haematopoietic stem cell transplantation (HSCT). Monitoring of haematopoietic donor/recipient chimerism is of major significance in detecting and quantifying engraftment or graft rejection of the donor-derived haematopoietic cells after transplantation. Radioactive analyses of polymorphic microsatellite markers are commonly used for chimerism analyses. We describe an improved, non-isotopic method that is based on the analysis of microsatellite markers in donor and recipient cells using capillary electrophoresis and fluorescence detection. Artificial mixtures of donor and recipient DNA that were generated from peripheral blood mononuclear cells from dog leukocyte antigen-identical siblings were used to analyse the sensitivity of the assay. DNA from dogs that had received HSCT were also analysed in order to demonstrate the feasibility of the method in vivo. For chimerism analyses, six different microsatellite loci were systematically amplified using fluorescent PCR primer. The fluorescent polymerase chain reaction products were separated by capillary electrophoresis using POP4 on a 310 ABI Prism Genetic Analyzer. After electrophoresis, fluorescence signals were automatically sized and quantified using GeneScan software. The method described provides an accurate assessment of haematopoietic chimerism in the canine model with significantly reduced hands-on time compared to conventional gel electrophoresis. 相似文献
93.
用350只1周龄的Arbor Acres(AA)商品肉公鸡进行试验,采用2×3+1试验设计,其中葡萄糖酸钠2个水平(0.2%),植酸酶3个水平(0.500、1000 U/kg),另有一满足NRC钙、磷需要的正常日粮,共7个处理,每个处理5个重复,每个重复10只鸡。研究玉米-豆粕型低磷日粮中添加葡萄糖酸钠和植酸酶对肉鸡磷利用的影响。试验期1~3周。结果表明,在低磷日粮中添加葡萄糖酸钠显著提高了肉鸡的钙(P=0.003)和磷(P<0.001)的表观存留率,降低了血清碱性磷酸酶活性(P=0.038);植酸酶提高了肉鸡的胫骨灰分重量(P<0.001)、钙(P=0.005)和磷(P<0.001)的表观存留率。在胫骨灰分重量(P=0.030)和磷表观存留率(P=0.008)上,葡萄糖酸钠和植酸酶存在互作。 相似文献
94.
95.
探讨了H_2O、1%SDS高温极短时间抽提丝胶和LiBr、NaSCN、CaCl_2浓溶液溶解丝胶对丝胶的破坏性。用SDS—聚丙烯酰胺凝胶电泳法分析了丝胶的肽链组分和分子量,归纳出丝胶含有约25种肽链,它们的分子量约从14000到256000。指出丝胶分子中含有两条肽链通过二硫键连结的结构。 相似文献
96.
利用聚丙烯酰胺垂直板凝胶电泳技术对南农选系及16 个当前生产上应用较多的草地早熟禾品种的过氧化物酶同工酶进行了研究。结果表明,供试材料共在18 个位点表现酶带。南农选系表现出13条酶带,除与其它16个品种所共有的6 条主酶带外,还出现了其特有的7 条酶带。对不同品种POD同工酶酶谱信息进行相异性类平均聚类分析,17 个供试品种(系)聚为3 类,其中南农选系、美国品种“Merit”及其它15个品种分别各成一类。据此推测,南农选系与其它供试品种的亲缘关系相对较远,为一个相对独立的品系。 相似文献
97.
We investigated the physicochemical properties of the thermal gel of water‐washed pork meat (WWM) in the presence of the soluble fraction of porcine sarcoplasmic protein (SP) obtained with ammonium sulfate at 75 percent saturation. Two precipitated fractions of SP were obtained at 0–50 percent and 50–75 percent saturation, named SP‐f1 and SP‐f2, respectively, and the soluble fraction obtained at 75 percent saturation, SP‐f3, was used. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis showed that SP‐f3 contained mainly glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), while SP‐f1 and SP‐f2 had other SPs such as phosphorylase b, enolase, actin and phosphoglycerate mutase. The gel strength of WWM was greater when SP‐f3 rather than one of various animal proteins such as bovine plasma (BP), egg white, or whey protein isolates (WPI), was added and SP‐f3 had a gel‐enhancing effect as good as that of polyphosphate (PP). The gel strength of WWM with added SP‐f3 increased significantly with NaCl at 0.15 mol/L or more, but not in the absence of NaCl (0 mol/L). The effect of SP‐f3 was evident at neutral pH and maximum gel strength was obtained at a pH above 6.0. Differential scanning calorimetric (DSC) analysis showed that an endothermic peak corresponding to myosin heads in WWM shifted to a lower temperature with the addition of SP‐f3, as in the case of PP, though there was no such shift in the presence of other animal proteins (BP, egg white and WPI), suggesting that SP‐f3 increases the gel strength of WWM through the dissociation of actomyosin similar to PP. Scanning electron microscopy (SEM) revealed wall‐like structures among the protein strands in the WWM gel matrix in the presence of SP‐f3. The results of DSC and SEM indicated that the formation of a gel network in meat products is reinforced with GAPDH in SP after the interaction between GAPDH and myofibrillar protein. 相似文献
98.
Yu Liu Wenhua Zhao Jianfeng Xu Xiaolin Yu Chun Ye Shulin Fu Yinsheng Qiu 《Journal of veterinary pharmacology and therapeutics》2019,42(5):580-584
The purpose of this study was to determine the pharmacokinetics of baicalin after intravenous and intramuscular administration of sodium baicalin at 50 mg/kg to piglets. Plasma baicalin levels were determined by high‐performance liquid chromatography. The plasma concentration–time data of baicalin for both administration routes were best described by two‐compartmental open model. The area under the plasma concentration–time curve and the elimination half‐lives were 77.47 ± 6.14 µg/ml × h and 1.73 ± 0.16 hr for intravenous and 64.85 ± 5.67 µg/ml × h and 2.42 ± 0.15 hr for intramuscular administration, respectively. The apparent volume of distribution and body clearance were 1.63 ± 0.23 L/kg and 2.74 ± 0.30 L h?1 kg?1 for intravenous and 0.51 ± 0.10 L/kg and 0.78 ± 0.08 L h?1 kg?1 for intramuscular routes, respectively. An intramuscular injection of sodium baicalin in piglets resulted in rapid and complete absorption, with a mean maximal plasma concentration of 77.28 ± 7.40 µg/ml at 0.17 hr and a high absolute bioavailability of 83.73 ± 5.53%. 相似文献
99.
100.
P Liberg 《Acta veterinaria Scandinavica》1977,18(3):335-348
In 283 dairy cows suffering from internal disorders the serum proteins were studied by agarose gel electrophoresis supplemented by total protein and albumin determinations. The clinical diagnoses could be grouped according to protein pattern. Group 1 (abomasal displacement and traumatic muscle injury) did not appreciably affect the serum protein concentrations and represented primarily non-inflammatory diseases or diseases of non-infectious origin. Group 2 (leukosis) occupied an exceptional position, with heavy lowering of albumin unaccompanied by a corresponding rise of total globulin. The γ-globulin concentration was significantly lowered. Group 3 (acute traumatic peritonitis) represented an acute inflammatory process with increase chiefly of the α-globulins, while the γ-globulin concentration was normal. Group 4 (chronic traumatic peritonitis, summer mastitis, chronic subclinical mastitis, chronic laminitis and polyarthritis, urinary tract infection, abscess, and sub-acute-chronic pneumonia) comprised diseases chiefly of chronic inflammatory character and with infectious origin. Especially characteristic was the heavy rise of globulins in general and of γ-globulin in particular. In most cases there was a large increase also of the α- and p-globulin fractions. 相似文献