北川白山羊基因组与表型性状相关的多态性RAPD标记

北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量（P=0.028）、体高（P=0.017）和体长（P=0.037）具有显著影响,SBS02对体质量（P=0.033）和体高（P=0.034）具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。     

土壤pH值对极小种群毛枝五针松生理特性的影响

幼苗建成期是毛枝五针松(Pinus wangii)天然更新的关键阶段,为找出影响毛枝五针松幼苗存活的关键生态因子,采用植物生理学方法,研究在7个酸碱度土壤培育下,毛枝五针松松针超氧化物歧化酶(SOD)、过氧化物酶活性(POD)活性和脯氨酸、可溶性糖质量分数及丙二醛(MDA)摩尔质量浓度的变化。结果表明:毛枝五针松在弱碱土壤中抗逆性更强,形态观察得知其在弱碱性土壤中生长更好;生理指标测定结果表明,土壤在p H=7.69~8.42时最适宜毛枝五针松幼苗的生长发育。     

Use of Porcine Meal in Plant‐based Practical Diets for Pacific White Shrimp,Litopenaeus vannamei

Two growth trials were conducted to evaluate and confirm the efficacy of a porcine meal (PM) with high protein content (>90%) as an alternative feed ingredient in commercial‐type feed formulation for Pacific white shrimp, Litopenaeus vannamei. Six experimental diets were formulated for the two growth trials. The first five diets contained increasing levels (0, 1, 2, 4, and 6%) of PM as a replacement for soybean meal in a plant‐based diet with low inclusion level (6%) of fish meal (FM). The last experimental diet was produced utilizing 4.2% PM to completely replace FM. In Trial 1, shrimp (1.5 g initial mean weight, 20 shrimp/tank, n = 4) were offered test diets for 6 wk in a semirecirculation system. At the end of Trial 1, shrimp fed with the diet containing 6% PM exhibited significantly enhanced weight gain (WG), feed conversion ratio (FCR), and survival compared to those fed with the diet devoid of FM. As survival was poor across all treatments and different densities could mask growth results the trial was repeated. In Trial 2, shrimp (0.85 g, 15 shrimp/tank, n = 4) were offered diets for 6 wk. Dietary supplementation of PM at 6% significantly improved WG, FCR, and apparent net protein retention in contrast with the treatment devoid of FM, confirming the same trends in Trial 1. No significant difference was detected in protein, lipid, moisture, and mineral profiles of whole‐body shrimp as well as survival across all the treatments. Results of this study indicate that PM is a good high protein source in shrimp feeds, which can be included up to 6% in the low FM‐based diet without compromising the growth of shrimp.     

某高铁隧道小口径井抽水试验探讨

指出了在某高铁隧道勘察中采用小口径抽水试验方法,具有操作简单便捷,成本低的优势。通过小口径井抽水试验查明场地的水文地质条件,得出了水文地质原始数据资料,根据单孔稳定流承压水非完整井的水文地质实际情况,选取合适的公式分别计算出了隧道岩层渗透系数及影响半径,为高铁隧道的勘察设计及顺利施工提供参考。     

紧压白茶品质安全性评价

为了探明紧压白茶饮用的安全性,以不同年份和花色的紧压白茶为原料,选用国家标准方法测定其主要的品质安全性指标,并进行分析和评价。结果表明:不同年份和花色的紧压白茶中未测出致病菌,其中1个样品检出霉菌;农药残留符合GB2763-2016食品中污染物限量要求;重金属检测中,铅含量0.229~0.819 mg/kg,砷含量0.071~0.205 mgkg,镉含量0.006~0.036 mg/kg,铬含量0.111~6.522 mg/kg,铜含量9.892~23.399 mg/kg,铝含量126.717~668.417 mg/kg,汞含量0~0.024 mg/kg,符合食品中污染物限量要求;理化检测中,水分含量4.6%~10.5%,灰分含量4.6%~5.7%,水浸出物含量30.2%~40.1%,茶梗含量0.8%~4.5%。由此可得出结论:紧压白茶的品质安全性可靠。     

极小种群野生植物八蕊单室茱萸的种群状况研究

八蕊单室茱萸为云南省特有的极小种群野生植物和濒危物种。通过查阅相关文献并结合访问调查,确定八蕊单室茱萸可能分布的区域,开展种群资源调查。结果显示:八蕊单室茱萸目前仅在普洱市和西双版纳州有10个天然种群,其种群规模已低于最小可存活种群,属极小种群野生植物和濒危物种,亟需开展保护工作。生境破坏和生境片段化是导致八蕊单室茱单室茱萸濒危的人为因素。提出收集种质资源、营建和管护近地保护种群、建立迁地和回归种群等保育建议。     

春白萝卜新品种雪单3 号的选育

雪单3 号是利用双单倍体育种技术选育的春白萝卜新品种。母本ED0108A 是以梅花春萝卜× 白玉春萝卜进行花药培
养获得的DH 系为轮回父本，与不育系进行多次回交，转育成的雄性不育系。父本ED3128 是由迟花春萝卜× 天鸿春萝卜进行
花药培养获得的DH 系。该品种生育期60 d（天），植株开展度45 cm，裂叶，小叶11 对，叶片数20，叶簇平展，叶色深绿；
肉质根长圆柱形，白皮白肉，根长36 cm，横径7.1 cm，单根质量1.1 kg，高抗黑腐病、芜菁花叶病毒病、黄瓜花叶病毒病和花
椰菜花叶病毒病，抗霜霉病，群体整齐度高。每667 m² 产量4 000～5 000 kg。适宜在湖北、湖南、河南、山东等地区早春栽培。